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Plant Physiology:CLE19在花粉外壁形成中的作用

已有 4320 次阅读 2017-9-22 09:11 |个人分类:每日摘要|系统分类:论文交流|关键词:学者

Cytological and Transcriptomic Analyses Reveal Important Roles of CLE19 in Pollen Exine Formation

First author:Shuangshuang Wang; Affiliations: Fudan University (复旦大学): Shanghai, China

Corresponding author: Fang Chang (常芳)

The CLAVATA3/ESR-RELATED (CLE) peptide signals are required for cell-cell communication in several plant growth and developmental processes. However, little is known regarding possible functions of the CLEs in the anther. Here, we show that a T-DNA insertional mutant, and dominant-negative (DN; 显性负性) and overexpression (OX) transgenic plants of the CLE19 gene exhibited significantly reduced anther size and pollen grain number, and abnormal pollen wall formation. Interestingly, the DN-CLE19 pollen grains showed a more extensively covered surface but CLE19-OX pollen exine exhibited clearly missing connections in the network and lacked separation between areas that normally form the lacunae (腔隙). With a combination of cell biological, genetic, and transcriptomic analyses on cle19, DN-CLE19, and CLE19-OX plants, we demonstrated that CLE19-OX plants produced highly vacuolated (有空泡的) and swollen (膨胀的) ams-like tapetal cells (绒毡层细胞), lacked lipidic tapetosomes (含油体) and elaioplasts (油粒体), and had abnormal pollen primexine (初生外壁) without obvious accumulation of sporopollenin (孢粉素) precursors. Moreover, CLE19 is important for the normal expression of > 1,000 genes, including transcription factor gene AMS, 280 AMS-downstream genes, and other genes involved in pollen coat (花粉外被) and pollen exine (花粉外壁) formation, lipid metabolism, pollen germination, and hormone metabolism. In addition, the DN-CLE19(+/+) ams(-/-) plants exhibited the ams anther phenotype and ams(+/-) partially suppressed the DN-CLE19 transgene-induced pollen exine defects. These findings demonstrate that the proper amount of CLE19 signal is essential for the normal expression of AMS and its downstream gene networks in the regulation of anther development and pollen exine formation.

CLE多肽信号对于某些植物生长和发育进程中细胞与细胞间的交流是至关重要的。然而,对于CLE在花药中可能起到的作用还不清楚。本文,作者通过对CLE19基因进行T-DNA插入突变、显性负性和过表达,结果导致花药尺寸的显著减小,花粉粒数量的降低,异常花粉壁的形成。有趣的事,DN-CLE19的花粉粒显示更广泛的表面覆盖,而CLE19-OX的花粉外壁存在明显的网络失联,在正常形成腔隙的位置并未分隔开。通过结合细胞生物学、遗传学和转录组对cle19DN-CLE19CLE19-OX植株进行分析,结果显示CLE19-OX植株产生了更加有空泡的并膨胀的类似ams突变体的绒毡层细胞,缺失了含油体和油粒体,并且花粉初生外壁异常,没有可观测到的孢粉素前体的积累。另外,CLE19对于超过1000个基因的正常表达十分重要,包括转录因子AMS基因及280个AMS下游的基因,还有一些参与花粉外被和花粉外壁形成、油脂代谢、花粉萌发和激素代谢的基因。此外,DN-CLE19(+/+) ams(-/-)突变体植株表现出ams突变体的花药表型,而ams(+/-)可以部分抑制DN-CLE19转基因诱导的花粉外壁缺陷。这些发现显示适当的CLE19信号对于AMS及其下游基因网络在调控花药发育和花粉外壁形成时的正常表达是必要的。

通讯:常芳 (http://life.fudan.edu.cn/Data/View/2168)

个人简介:2009年,中国农业大学,植物学博士; 2006-2007年,美国加州大学河滨分校,植物系访问学者;2013年,澳洲昆士兰科技大学,访问学者;2009年-今,复旦大学生命科学学院,讲师、副教授。


doi: https://doi.org/10.1104/pp.17.00439

Journal: Plant Physiology
First Published data: September 21, 2017.

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