Identification of new protein–protein and protein–DNA interactions linked with wood formation in Populus trichocarpa
First author:H. Earl Petzold; Affiliations: Virginia Tech(弗吉尼亚理工大学): Blacksburg, USA
Corresponding author:Amy M Brunner
Cellular processes, such as signal transduction and cell wall deposition, are organized by macromolecule (高分子) interactions. Experimentally determined protein–protein interactions (PPIs) and protein–DNA interactions (PDIs) relevant to (有关的) woody plant development are sparse (稀少的). To begin to develop a Populus trichocarpa Torr. & A. Gray wood interactome (互作组), we applied the yeast-two-hybrid (Y2H) assay in different ways to enable the discovery of novel PPIs and connected networks. We first cloned open reading frames (ORFs) for 361 genes markedly upregulated in secondary xylem compared with secondary phloem and performed a binary Y2H screen with these proteins. By screening a xylem cDNA library for interactors of a subset of these proteins and then recapitulating the process by using a subset of the interactors as baits (诱饵), we ultimately identified 165 PPIs involving 162 different ORFs. Thirty-eight transcription factors (TFs) included in our collection of P. trichocarpa wood ORFs were used in a Y1H screen for binding to promoter regions of three genes involved in lignin biosynthesis resulting in 40 PDIs involving 20 different TFs. The network incorporating both the PPIs and PDIs included 14 connected subnetworks, with the largest having 132 members. Protein–protein interactions and PDIs validated previous reports and also identified new candidate wood formation proteins and modules through their interactions with proteins and promoters known to be involved in secondary cell wall synthesis. Selected examples are discussed including a PPI between Mps one binder (MOB1) and a mitogen-activated protein kinase kinase kinase kinase (M4K) that was further characterized by assays confirming the PPI as well as its effect on subcellular localization (亚细胞定位). Mapping of published transcriptomic data showing developmentally detailed expression patterns across a secondary stem onto the network supported that the PPIs and PDIs are relevant to wood formation, and also illustrated that wood-associated interactions involve gene products that are not upregulated in secondary xylem.
