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Nature Communications:拟南芥MIK2受体激酶通过感知微生物保守信号多肽诱导免疫
2021-9-21 23:21
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The Arabidopsis MIK2 receptor elicits immunity by sensing a conserved signature from phytocytokines and microbes

第一作者Shuguo Hou

第一单位山东建筑大学

第一通讯Ping He


 Abstract 


背景回顾Sessile plants encode a large number of small peptides and cell surface-resident receptor kinases, most of which have unknown functions. 


主要发现:Here, we report that the Arabidopsis receptor kinase MALE DISCOVERER 1-INTERACTING RECEPTOR-LIKE KINASE 2 (MIK2) recognizes the conserved signature motif of SERINE-RICH ENDOGENOUS PEPTIDEs (SCOOPs) from Brassicaceae plants as well as proteins present in fungal Fusarium spp. and bacterial Comamonadaceae, and elicits various immune responses. 


结果1-SCOOP依赖MIK2SCOOP signature peptides trigger immune responses and altered root development in MIK2-dependent manner with a sub-nanomolar sensitivity. 


结果2-SCOOP12与MIK2:SCOOP12 directly binds to the extracellular leucine-rich repeat domain of MIK2 in vivo and in vitro, indicating that MIK2 is the receptor of SCOOP peptides.


结果3-下游信号:Perception of SCOOP peptides induces the association of MIK2 and the coreceptors SOMATIC EMBRYOGENESIS RECEPTOR KINASE 3 (SERK3) and SERK4 and relays the signaling through the cytosolic receptor-like kinases BOTRYTIS-INDUCED KINASE 1 (BIK1) and AVRPPHB SUSCEPTIBLE1 (PBS1)-LIKE 1 (PBL1). 


结论:Our study identifies a plant receptor that bears a dual role in sensing the conserved peptide motif from phytocytokines and microbial proteins via a convergent signaling relay to ensure a robust immune response.


 摘 要 


固着植物会编码许多小肽以及细胞表面上的受体激酶,然而其中大多数的功能未知。本文中,作者报道了拟南芥中的受体激酶MIK2识别来自十字花科的SCOOPs以及真菌镰刀菌和细菌丛毛单胞菌中蛋白的保守信号基序,从而引起各种免疫响应。SCOOP信号多肽诱导免疫响应,并改变根的发育,该过程以亚纳摩尔级别的敏感性依赖于受体激酶MIK2。体内和体外实验均显示,SCOOP12能够直接结合到MIK2的胞外富含亮氨酸重复结构域,说明MIK2是SCOOP多肽的受体。对于SCOOP的感知诱导MIK2与共受体SERK3和SERK4的关联,并通过胞质类受体激酶BIK1和PBL1来继续往下传递信号。本文的研究鉴定了一个植物受体,其能够感知植物细胞因子和微生物蛋白的保守多肽基序,并通过一个收敛性信号转导中继,保证植物中强健免疫响应的建立。


 通讯作者 

** Ping He **


个人简介:

1993年,中国农业大学,学士;

1998年,中国科学院,硕士;

2003年,堪萨斯州立大学,博士;

2003-2008年,哈佛医学院,博后。


研究方向植物免疫激活与信号转导机制


doi: https://doi.org/10.1038/s41467-021-25580-w


Journal: Nature Communications

Published dateSeptember 17, 2021

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