背景+问题：Cryptochromes (CRYs) are evolutionarily conserved photolyase-like photoreceptors found in almost all species, including mammals. CRYs regulate transcription by modulating the activity of several transcription factors, but whether and how they affect pre-mRNA processing are unknown. Photoperiod and temperature are closely associated seasonal cues that influence reproductive timing in plants. CRYs mediate photoperiod-responsive floral initiation, but it is largely unknown whether and how they are also involved in thermosensory flowering. 

主要发现：We establish here that blue light and CRY2 play critical roles in thermosensory flowering in Arabidopsis thaliana by regulating RNA alternative splicing (AS) to affect protein expression and development. 

结果1-CIS1和CRY2互作：CRY2 INTERACTING SPLICING FACTOR 1 (CIS1) interacts with CRY2 in a blue light–dependent manner and promotes CRY2–mediated thermosensory flowering. 

结果2-FLM基因可变剪切：Blue light, CRYs, and CISs affect transcriptome-wide AS profiles, including those of FLOWERING LOCUS M (FLM), which is critical for temperature modulation of flowering. 

结果3-CIS1调控FLM的可变剪切：Moreover, CIS1 binds to the FLM pre-mRNA to regulate its AS, while CRY2 regulates the RNA-binding activity of CIS1. 

结论：Thus, blue light regulates thermosensory flowering via a CRY2–CIS1–FLM signaling pathway that links flowering responses to both light and ambient temperature.

A hypothetical model for blue-light regulation of thermosensory flowering via the CRY2–CIS1–FLM signaling pathway.

 摘 要 

隐花色素CRYs是一类演化上保守的光裂合酶类光受体，在包括动物在内的所有物种中都有发现。CRYs通过影响一些转录因子的活性来调控转录，但是其是否会以及如何影响mRNA前体加工的还不清楚。光周期和温度是两个极度相关的季节因素，影响着植物的生殖时间。CRYs介导光周期相应的成花起始，但是仍不清楚其是否会以及如何参与到温敏开花。本文中，作者发现蓝光和CRY2通过调控RNA可变剪切来影响蛋白表达和发育， 从而在拟南芥的温敏开花中发挥重要作用。与CRY2互作的剪接因子CIS1能够以一个蓝光依赖性的方式与CRY2的互作，从而促进CRY2介导的温敏开花。蓝光、CRYs和CISs影响转录组范围上的可变剪切谱，其中就包括了开花温度调控的关键基因FLM。此外，CIS1能够结合到FLM基因mRNA前体上调控该基因的可变剪切，而CRY2能够调控CIS1的RNA结合活性。因此， 蓝光通过一个CRY2–CIS1–FL信号转导途径调控植物的温敏开花，从而将开花与光和环境温度联系在一起。