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PNAS:TOR信号转导结合能量和氧两条感知途径,调控拟南芥缺氧响应基因表达

已有 1264 次阅读 2023-1-12 15:26 |个人分类:每日摘要|系统分类:论文交流

Target of rapamycin signaling couples energy to oxygen sensing to modulate hypoxic gene expression in Arabidopsis

第一作者Alicja B. Kunkowska

第一单位意大利圣安娜高等研究学院

通讯作者Pierdomenico Perata


 Abstract 

背景1-缺氧响应基因:Plants respond to oxygen deprivation by activating the expression of a set of hypoxia-responsive genes (HRGs). The master regulator of this process is a small group of transcription factors belonging to group VII of the ethylene response factors (ERF-VIIs). 


背景2-植物半胱氨酸氧化酶ERF-VIIs are highly unstable under aerobic conditions due to the continuous oxidation of their characteristic Cys residue at the N terminus by plant cysteine oxidases (PCOs). Under hypoxia, PCOs are inactive and the ERF-VIIs activate transcription of the HRGs required for surviving hypoxia. 


问题及假设-糖/能量感知途径However, if the plant exposed to hypoxia has limited sugar reserves, the activity of ERF-VIIs is severely dampened. This suggests that oxygen sensing by PCO/ERF-VII is fine-tuned by another sensing pathway, related to sugar or energy availability. 


主要发现Here, we show that oxygen sensing by PCO/ERF-VII is controlled by the energy sensor target of rapamycin (TOR). 


结果1-TOR通过磷酸化并激活ERF-VII蛋白,增强ERF-VII介导的HRGs转录激活Inhibition of TOR by genetic or pharmacological approaches leads to a much lower induction of HRGs. We show that two serine residues at the C terminus of RAP2.12, a major ERF-VII, are phosphorylated by TOR and are needed for TOR-dependent activation of transcriptional activity of RAP2.12. 


结论1Our results demonstrate that oxygen and energy sensing converge in plants to ensure an appropriate transcription of genes, which is essential for surviving hypoxia. 


结果2-低氧减少ATP,降低TOR活性,减弱ERF-VII介导的HRGs转录激活When carbohydrate metabolism is inefficient in producing ATPbecause of hypoxia, the lower ATP content reduces TOR activity, thus attenuating the efficiency of induction of HRGs by the ERF-VIIs


结论2This homeostatic control of the hypoxia-response is required for the plant to survive submergence.


image.png

植物TOR复合体核心组分RAPTOR和LST8功能缺失突变体在缺氧胁迫下的表型


 摘 要 

植物能够通过激活一系列的缺氧响应基因(HRGs)来响应缺氧环境。该过程主要的调控子是一小类属于乙烯响应因子(ERF)家族的亚群ERF-VIIs。该类转录因子在有氧条件下高度不稳定,主要是由于其N端的特征残基半胱氨酸(Cys)被植物半胱氨酸氧化酶(PCOs)连续的氧化。在缺氧条件下,PCOs酶失活,ERF-VIIs转录因子激活HRGs的转录,从而保证植物在缺氧条件下的生存。但是,如果在植物暴露在缺氧条件下时糖储量有限,那么ERF-VIIs的活性就会受到严重的抑制。这说明PCO/ERF-VII介导的氧感知受到另外一个与糖或能量相关感知途径的精确调控。本文中,作者发现PCO/ERF-VII介导的氧感知受到能量感知器TOR的控制。通过遗传和药理学抑制TOR活性,会导致HRGs诱导水平的降低。作者发现ERF-VII主要转录因子RAP2.12蛋白C端的两个丝氨酸残基受到TOR的磷酸化,并且这对于RAP2.12依赖于TOR的转录活性激活是必需的。本文的研究结果表明植物中氧感知和能量感知途径会聚与一处,从而保证特定基因的转录激活,最终使得植物在缺氧条件下能够顺利存活。由于缺氧导致碳水化合物代谢产生的ATP不足,低水平的ATP含量会导致TOR活性的降低,从而减弱其对ERF-VIIs介导的HRGs转录的诱导效率。这一对缺氧响应的稳态控制是植物在淹水条件下保证生存所必需的。




** Pierdomenico Perata **


个人简介:

1985年,意大利比萨大学,硕士;

1990年,意大利比萨大学,博士;

1991-1999年,意大利比萨大学,助理教授;

1999-2000年,巴里大学,副教授;

2000年,摩德纳-雷焦·艾米里亚大学,教授。


研究方向:植物中缺氧响应的分子生理,阐明植物中的氧感知和信号转导机制。


doi: https://doi.org/10.1073/pnas.2212474120


Journal: PNAS

Published date: January 10, 2023


Cite:
Alicja B. Kunkowska, Fabrizia Fontana, Federico Betti, Raphael Soeur, Gerold J. M. Beckers, Christian Meyer, Geert De Jaeger, Daan A. Weits, Elena Loreti, Pierdomenico Perata. Target of rapamycin signaling couples energy to oxygen sensing to modulate hypoxic gene expression in Arabidopsis. PNAS, 2022, 120(3): e2212474120. DOI: https://doi.org/10.1073/pnas.2212474120




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