TickingClock的个人博客分享 http://blog.sciencenet.cn/u/TickingClock

博文

Plant Physiology:野草莓中4个LFY基因拷贝在花模式建成和叶发育中的作用

已有 1251 次阅读 2023-2-8 22:25 |个人分类:每日摘要|系统分类:论文交流

Roles and evolution of four LEAFY homologs in floral patterning and leaf development in woodland strawberry

第一作者Yunming Zhang

第一单位华中农业大学

通讯作者Chunying Kang


 ABSTRACT 

背景回顾:The plant-specific transcription factor LEAFY (LFY), generally maintained as a single copy gene in most angiosperm species, plays critical roles in flower development. The woodland strawberry (Fragaria vesca) possesses four LFY homologs in the genome; however, their respective functions and evolution remain unknown.


结果1-FveLFYa/b/c/d的功能Here, we identified and validated that mutations in one of the four LFY homologs, FveLFYa, cause homeotic conversion of floral organs and reiterative outgrowth of ectopic flowers. In contrast to FveLFYaFveLFYb/c/d appear dispensable under normal growth conditions, as fvelfyc mutants are indistinguishable from wild type and FveLFYb and FveLFYd are barely expressed. 


结果2-草莓和拟南芥LFY功能保守性Transgenic analysis and yeast one-hybrid assay showed that FveLFYa and FveLFYb, but not FveLFYc and FveLFYd, are functionally conserved with AtLFY in Arabidopsis (Arabidopsis thaliana). 


结果3-FveLFY结合位点Unexpectedly, LFY binding site prediction and yeast one-hybrid assay revealed that the transcriptional links between LFY and the APETALA1 (AP1) promoter/the large AGAMOUS (AG) intron are missing in F. vesca, which is due to the loss of LFY binding sites. The data indicate that mutations in cis-regulatory elements could contribute to LFY evolution


结果4-FevLFYa参与叶发育Moreover, we showed that FveLFYa is involved in leaf development, as approximately 30% of mature leaves have smaller or fewer leaflets in fvelfya


结果5-草莓属LFY基因演化Phylogenetic analysis indicated that LFY homologs in Fragaria species may arise from recent duplication events in their common ancestor and are undergoing convergent gene loss. 


结论Together, these results provide insight into the role of LFY in flower and leaf development in strawberry and have important implications for the evolution of LFY.

image.png


 摘 要 

在花发育中发挥重要作用的植物特异性转录因子LEAFY,在大多数的被子植物物种中均保持为单拷贝基因。野草莓(Fragaria vesca)基因组中编码了4个LFY同源基因,但是其各自的功能及演化仍不清楚。本文中,作者发现其中一个LFY基因FveLFYa突变会导致花器官的同源异型转化以及异位花的反复生长。与FveLFYa基因相反,正常条件下FveLFYb/c/d基因并非必需,具体表现为fvelfyc突变体与野生型相差无几且FveLFYbFveLFYd几乎不表达。遗传转化和酵母单杂试验表明FveLFYaFveLFYb与拟南芥的AtLFY基因功能保守,但是FveLFYcFveLFYd基因不是。出乎意料地,LFY结合位点预测和酵母单杂试验表明,野草莓中AP1启动子和AG基因大段内含子中并不包含LFY的结合位点,因此他们之间不存在转录调控关联。这些数据表明,顺式调控元件的突变能够作用于LFY的演化。此外,作者还发现FveLFYa参与了叶片的发育,在fvelfya突变体中大约有30%的成熟叶片更小或者小叶更少。系统发育分析显示,草莓属物种中LFY同源基因可能起源于其共同祖先在近期发生的复制事件,并且经历了趋同性的丢失。综上,本文的研究结果揭示了草莓LFY基因在花和叶发育中的功能,并且对于LFY基因的演化提供了新的视野。



** 康春颖 **


个人简介:

2000-2004年,中国农业大学,学士;

2004-2010年,中科院上海植物生理生态研究所,博士;

2010-2014年,美国马里兰大学,博后;

2014年-至今,华中农业大学,教授。


研究方向:通过化学诱变获得二倍体森林草莓的突变群体,结合生物信息学、分子生物学、发育生物学、生物化学等多种实验手段挖掘和解析草莓株型、花器官和果实发育的关键调控基因,用于草莓遗传改良和育种。


doi: https://doi.org/10.1093/plphys/kiad067 


Journal: Plant Physiology

Published date: February 03, 2023


Cite:
Yunming Zhang, Lijun Kan, Shaoqiang Hu, Zhongchi Liu, Chunying Kang, Roles and evolution of four LEAFY homologs in floral patterning and leaf development in woodland strawberry. Plant Physiology, 2023. DOI: https://doi.org/10.1093/plphys/kiad067



https://m.sciencenet.cn/blog-3158122-1375436.html

上一篇:The Plant Cell:GRAS转录因子SCL5通过激活DOF3.4,促进细胞增殖和根组织再生
下一篇:Nature Plants:F-box蛋白UFO通过控制LFY的结合元件,调控植物花发育

0

该博文允许注册用户评论 请点击登录 评论 (0 个评论)

数据加载中...

Archiver|手机版|科学网 ( 京ICP备07017567号-12 )

GMT+8, 2024-3-29 15:08

Powered by ScienceNet.cn

Copyright © 2007- 中国科学报社

返回顶部