氢气生物学效应研究方面，植物效应研究中国学者，尤其是南京农业大学独树一帜，最近崔瑾教授课题组连续发表氢对蔬菜品质影响的研究，刚刚在线发表一篇论文证明，氢气水可以提高常见蔬菜，小白菜能耐受重金属镉污染，减少蔬菜中重金属水平，提高蔬菜抗氧化物质水平。研究说明，氢水在蔬菜生产中具有应用前景。

Hydrogen-rich water enhances cadmium tolerance in Chinese cabbage by reducing ca.pdf

Fig. 2. HRW pretreatment alleviated Cd stress-induced Chinese cabbage seedlings growth inhibition. The seedlings in solution were supplemented with 0 or 50% saturationof HRW for 48 h, followed by another 24 h incubation in 0 or 50 _M CdCl2. Bar = 3 cm (A). Changes in root length (B) and fresh weight (C) during the 24 h treated with 0 or50 _M CdCl2. Data are means ± SE from three independent experiments. Bars with different letters are significantly different at P < 0.05 according to Duncan’s multiple rangetest.

Fig. 4. Cadmium localization in root tissues of Chinese cabbage. The roots were pretreatment with 0 or 50% saturation of HRW solution for 48 h, followed by exposure to 0or 50 _M CdCl2for another 24 h. Bar = 20 _m. Arrows point to precipitates of cadmium–dithizone.

J Plant Physiol. 2014 Nov 18;175C:174-182. doi: 10.1016/j.jplph.2014.09.017. [Epub ahead of print]

Hydrogen-rich water enhances cadmium tolerance in Chinese cabbage by reducing cadmium uptake and increasing antioxidant capacities.

Wu Q1, Su N1, Cai J1, Shen Z1, Cui J2.

Author information1College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.2College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: cuijin@njau.edu.cn.

The aim of the present paper was to understand the specific mechanism of hydrogen-rich water (HRW) in alleviating cadmium (Cd) toxicity in Chinese cabbage (Brassica campestris spp. chinensis L.). Our results showed that the addition of 50% saturation HRW significantly alleviated the Cd toxic symptoms, including the improvement of both root elongation and seedling growth inhibition. These responses were consistent with a significant decrease of Cd accumulation in roots and shoots, which was further confirmed by the histochemical staining. Molecular evidence illustrated that Cd-induced up-regulations of IRT1 and Nramp1 genes, responsible for Cd absorption, were blocked by HRW. By contrast, Cd-induced up-regulation of the HMA3 gene, which regulates Cd sequestration into the root vacuoles, was substantially strengthened by HRW. Furthermore, compared with those in Cd stress alone, the expressions of HMA2 and HMA4, which function in the transportation of Cd to xylem, were repressed by co-treatment with HRW. HRW enhanced the activities of antioxidant enzymes, including superoxide dismutase, guaiacol peroxidase, catalase and ascorbate peroxidase. These results were further confirmed by the alleviation of oxidative damage, as indicated by the decrease of thiobarbituric acid reactive substances (TBARS) and reactive oxygen species (ROS) production. Taken together, these results suggest that the improvement of Cd tolerance by HRW was associated with reduced Cd uptake and increased antioxidant defense capacities. Therefore, the application of HRW may be a promising strategy to improve Cd tolerance of Chinese cabbage.