博文
Nature_Dissect the bread wheat genome
|||
- Dissect the bread wheat genome using next-generation sequencing.
- The sequence data do cover nearly all wheat genes.
- The size of wheat genome is about 17 gigabase pairs.
- About 80% of the genome sequences are repetitive sequences.
- 94,000-96,000 genes were identified, and this number is consistent with the number of genes identified from A, B and D genome (T. monococcum and Ae. speltoides andAe. tauschii).
- Based on the sequence data from T. monococcum and Ae. speltoides and Ae. tauschii, 2/3 of these identified genes were assigned to A, B and D genome.
- Using the full-length cDNA sequences from rice, sorghum, brachypodium and barley, the 454 reads of bread wheat were assembled and 949,279 sequences were identified.
- By comparing these sequences with the Brachypodium genome, the sequence conservation was investigated. Brachypodium sequence on 1 and 4 chromosomes showed less conservative with sequences from bread wheat.
- Generally, genes lost during the polyploidy process. But several class of gene families with the role of defence, nutrition content, energy metabolism and growth have increased size (due to domestication).
I think, the major contribution of this work is that it produced nearly all the wheat genome sequence and assembled nearly all gene sequences. But no physical map, no location of these genes.
It is much easier to get the copy number of certain gene in bread wheat.
Here is the report from Scientific American_"
New Slice of Wheat Genome Could Help Feed Growing Global Population"http://blogs.scientificamerican.com/observations/2012/11/28/new-slice-of-wheat-genome-could-help-feed-growing-global-population/
https://m.sciencenet.cn/blog-508050-641795.html
上一篇：Unix and R
下一篇：The Tale of the TALEs（update）
