Statistical Analysis of the Benefits of Focused Saturation Mutagenesis in Directed Evolution Based on Reduced Amino Acid Alphabets 基于约化氨基酸字母表的定向进化中聚焦饱和突变效益的统计分析 来源: ACS catalysis IF : 12.350 摘要:定向进化技术是改造酶催化特性(如立体 / 区域 / 化学选择性、活 ...
Auto- and Hetero-Catalytic Processing of the N-Terminal Propeptide Promotes the C-Terminal Fibronectin Type III Domain-Mediated Dimerization of a Thermostable Vpr-like Protease N 端前到肽的自催化和异催化处理促进 C 端纤连蛋白 III 型结构域介导的嗜热 Vpr 样蛋白酶的二聚体化 来源: AEM IF : 5 ...
Molecular Evolution of an Aminotransferase Based on Substrate–Enzyme Binding Energy Analysis for Efficient Valienamine Synthesis 基于酶 - 底物结合能分析氨基转移酶的分子进化,用于高效合成井冈霉烯胺 来源: ACS catalysis IF : 12.350 摘要:井冈霉烯胺是活性药物和农用化学品的宝贵组成部分,因为 ...
Co-Deletion of A238L and EP402R Genes from a Genotype IX African Swine Fever Virus Results in Partial Attenuation and Protection in Swine IX 型非洲猪瘟病毒共删除 A238L 和 EP402R 基因导致猪的部分减毒和保护 来源: Viruses IF : 5.818 摘要:非洲猪瘟病毒( ASFV )是非洲猪瘟( ASF )的病原体, ...
Engineering cascade biocatalysis Chock for in whole cells for bottom-up synthesis of cello-oligosaccharides: flux control over three enzymatic steps enables soluble production 通过工程级联生物催化在全细胞中自下而上地合成纤维寡糖:对三个酶的通量控制实现了可溶性生产 来源: Microbial Cell Factorie ...
柠檬烯环氧化物水解酶突变体在立体选择性变换中的催化机制在结构和计算方面的启示 Structural and Computational Insight into the Catalytic Mechanism of Limonene Epoxide Hydrolase Mutants in Stereoselective Transformations 来源:JACS IF:16.383 摘要:柠檬烯环氧化物水解酶(LEH)的定向进化催化环戊烯氧化 ...
大肠埃希菌 C321. △ A 转录组和蛋白质组的比较分析及通过整合 T7 RNA 聚合酶进一步提高其非经典氨基酸的蛋白质表达能力 Comparative Analyses of the Transcriptome and Proteome of Escherichia coli C321. △ A and Further Improving Its Noncanonical Amino Acids Containing Protein Expression Abilit ...
通过第二密码子工程调控微生物细胞活性提高化合物产量 Engineering microbial cell viability for enhancing chemical production by second codon engineering 来源: METABOLIC ENGINEERING IF : 8.829 摘要:微生物细胞工厂利用多种高端策略将可再生生物质原料发酵转化为可持续生产的工业化产品。然而 ...