The cryo-EM Structure of Thermotoga maritima β‑Galactosidase: Quaternary Structure Guides Protein Engineering 海栖热袍菌β-半乳糖苷酶的冷冻电镜结构:通过四级结构指导蛋白工程 来源:acs chemical biology IF:4.398 摘要:乳糖不耐症是一种常见的消化系统疾病,症状的严重程度差异 ...
Design of a programmable biosensor-CRISPRi genetic circuits for dynamic and autonomous dual-control of metabolic flux in Bacillus subtills 枯草芽孢杆菌代谢通量动态和自主双重控制的可编程生物传感器-CRISPRi遗传电路的设计 来源: Nucleic Acids Research IF:11.501 摘要: 动态 ...
基于anti-CRISPR蛋白的依赖细胞周期的CRISPR-Cas9激活系统改善基因编辑 A cell cycle-dependent CRISPR-Cas9 activation system based on an anti-CRISPR protein shows improved genome editing accuracy IF:4.166 来源:COMMUNICATIONS BIOLOGY 摘要: 基于Cas9核酸内切酶的基因组编辑系统的开发对于基 ...
CRISPR-guided DNA polymerases enable diversification of all nucleotides in tunable window 可变阅读框内,CRISPR引导DNA聚合酶实现核苷酸的多样化 来源:LETTER IF:1.5 摘要: 遗传密码的多样化帮助我们理解并改造生物系统。对于不能有效地利用同源重组的方式整合核苷酸的生物 ...
一株革兰氏阳性菌分泌的新型分支酸膜囊泡参与胞外血红素 回收与种间共享 Extracellular heme recycling and sharing across species by novel mycomembrane vesicles of a Gram-positive bacterium 来源: Multidisciplinary Journal of Microbial Ecology (The ISME Journal) IF: 9.18 ...
在毕赤酵母中构建可消除游离sgRNA质粒的高效CRISPR/Cas9基因组编辑系统 High efficiency CRISPR/Cas9 genome editing system with an eliminable episomal sgRNA plasmid in Pichia pastoris 来源 :Enzyme and Microbial Technology IF :3.448 摘要 :巴斯德毕赤酵母是一种甲醇营养酵母,由于其 ...