We present the synthesis, spectroscopic properties, and live-cell application of a new BODIPY (boron
dipyrromethene) fluorescence probe BOD-Se based on selenoxide spirocyclization reaction for peroxynitrite
detection. The probe employs BODIPY dye as fluorophore, and is integrated with a chemical
peroxynitrite responsive organoselenium functional group. By using reactive diaryl selenide and
modulating by intramolecular charge-transfer (ICT) process, the probe is employed for evaluating
intracellular peroxynitrite level changes. Different intracellular peroxynitrite levels can be detected with
BOD-Se by confocal microscopy experiments using mouse macrophage cell line RAW264.7.

A BODIPY fluorescence probe modulated by selenoxide spirocyclization reaction
for peroxynitrite detection and imaging in living cells
Bingshuai Wang 1, Fabiao Yu 1, Peng Li, Xiaofei Sun, Keli Han

Dyes and Pigments 96 (2013) 383-390