The truth about metagenomics: quantifying and counteracting bias in 16S rRNA studies 2015 （ http://www.ncbi.nlm.nih.gov/pubmed/25880246 ） 16S rRNA gene high-throughput sequencing data mining of microbial diversity and interactions 2015 （ http://www.ncbi.nlm.nih.gov/pubmed/25808518 ...
文章： A comprehensive benchmarking study of protocols and sequencing platforms for 16S rRNA community profiling 2016 （ http://www.ncbi.nlm.nih.gov/pubmed/26763898 ） 文章： Improved OTU-picking using long-read 16S rRNA gene amplicon sequencing and generic hierarchical clustering ...
Vikodak - A Modular Framework for Inferring Functional Potential of Microbial Communities from 16S Metagenomic Datasets 2016 ( https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4746064/ ) A n integrated strategy for functional analysis of microbial communities based on gene ontology and ...
文章： Studying long 16S rDNA sequences with ultrafast- metagenomic sequence classification using exact alignments (Kraken) 2016 （ https://www.ncbi.nlm.nih.gov/pubmed/26812576 ） 文章： A novel semi-supervised algorithm for the taxonomic assignment of metagenomic re ...
文章： Impact of library preparation protocols and template quantity on the metagenomic reconstruction of a mock microbial community 2015 （ http://www.ncbi.nlm.nih.gov/pubmed/26496746 ） 文章： Phasing amplicon sequencing on Illumina Miseq for robust environmental ...
文章：De novo clustering methods outperform reference-based methods for assigning 16S rRNA gene sequences to operational taxonomic units 2015 （ http://www.ncbi.nlm.nih.gov/pubmed/26664811 ） 文章： Comparing K-mer based methods for improved classification of 16S sequences 20 ...
FastQC: Quality control tool for high-throughput sequence data using modular options and giving graphic results of quality per base sequence, GC content, Nnumbers, duplication, and over represent ( http://www.bioinformatics.babraham.ac.uk/projects/fastqc/ ) Fastx-Toolkit: Command ...
PIC RSt (phylogenetic investigation of communities by reconstruction of unobserved states)， a computational approach to predict the functional composition of a metagenome using marker gene data and a database of reference genomes. 是预测样品所具有的功能，基于16s DNA和参考序 ...
文章： UCHIME improves sensitivity and speed of chimera detection 嵌合体产生的原因，主要是PCR的过程： The most common mechanism is incomplete template extension, when a partially extended sequence from one sequence reanneals to another parent in the next cycle of PCR. This pr ...
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