文章: Na ̈ıve Bayesian Classifier for Rapid Assignment of rRNA Sequencesinto the New Bacterial Taxonomy RDP Classifier是基于朴素贝叶斯的原理进行分类,其中关键需要计算后先验概率和条件概率。 1)其中选择8-bp的subsequences作为特征序列去计算相应的概率 ...
文章: Development of a Dual-Index Sequencing Strategy and CurationPipeline for Analyzing Amplicon Sequence Data on the MiSeqIllumina Sequencing Platform 测序平台越来越丰富,考虑选择测序平台的时候主要考虑如下因素: 1) We contend thatsequence quality is the most important ...
文章:Can we predict future allergies from our infant gut microbiota? Sensitization to food allergens is common during early life, affecting up to 28% of preschool children(学龄前的儿童多会发生食物过敏的情况). While 66 – 90% of infants outgrow their sensitization t ...
Primer and platform effects on 16S rRNA tag sequencing (http://www.ncbi.nlm.nih.gov/pubmed/26300854) 第一、微生物研究中主要的技术应用历程 第二、对于phix的比例在16s中一直未见到较为公认的数值,此文中描述 Varying PhiX spi ...
在16s rRNA的分析中,在数据处理过程中重要的一步操作是鉴定和去除嵌合体。 嵌合体(chimera)产生的原因主要是PCR过程中产生的错误: During this PCR amplification,chimeras might be created due to incomplete extension. 在扩增过程中,chimera的比例 ...