PIC RSt (phylogenetic investigation of communities by reconstruction of unobserved states)， a computational approach to predict the functional composition of a metagenome using marker gene data and a database of reference genomes. 是预测样品所具有的功能，基于16s DNA和参考序 ...
文章： UCHIME improves sensitivity and speed of chimera detection 嵌合体产生的原因，主要是PCR的过程： The most common mechanism is incomplete template extension, when a partially extended sequence from one sequence reanneals to another parent in the next cycle of PCR. This pr ...
A diversity index is a quantitative measure that reflects how many different types (such as species)there are in a dataset , and simultaneously takes into account how evenly the basic entities (such as individuals) are distributed among those types. The value of a diver ...
文章： Na ̈ıve Bayesian Classifier for Rapid Assignment of rRNA Sequencesinto the New Bacterial Taxonomy RDP Classifier是基于朴素贝叶斯的原理进行分类，其中关键需要计算后先验概率和条件概率。 1）其中选择8-bp的subsequences作为特征序列去计算相应的概率 ...
文章： Development of a Dual-Index Sequencing Strategy and CurationPipeline for Analyzing Amplicon Sequence Data on the MiSeqIllumina Sequencing Platform 测序平台越来越丰富，考虑选择测序平台的时候主要考虑如下因素： 1） We contend thatsequence quality is the most important ...
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