TickingClock的个人博客分享 http://blog.sciencenet.cn/u/TickingClock

博文

Cell:通过基因组编辑为作物改良提供数量性状变异

已有 4473 次阅读 2017-10-19 08:26 |个人分类:每日摘要|系统分类:论文交流|关键词:学者

Engineering Quantitative Trait Variation for Crop Improvement by Genome Editing


First author:Daniel Rodríguez-Leal; Affiliations: Cold Spring Harbor Laboratory (冷泉港实验室): NY, USA

Corresponding author: Zachary B. Lippman


Major advances in crop yields are needed in the coming decades. However, plant breeding is currently limited by incremental improvements in quantitative traits that often rely on laborious (费力的) selection of rare naturally occurring mutations in gene-regulatory regions. Here, we demonstrate that CRISPR/Cas9 genome editing of promoters generates diverse cis-regulatory alleles that provide beneficial (有益的) quantitative variation for breeding. We devised (设计) a simple genetic scheme, which exploits trans-generational heritability of Cas9 activity in heterozygous loss-of-function mutant backgrounds, to rapidly evaluate the phenotypic impact of numerous promoter variants for genes regulating three major productivity traits in tomato: fruit size, inflorescence branching (花枝), and plant architecture (株型). Our approach allows immediate selection and fixation (固定) of novel alleles in transgene-free plants and fine manipulation of yield components. Beyond a platform to enhance variation for diverse agricultural traits, our findings provide a foundation for dissecting complex relationships between gene-regulatory changes and control of quantitative traits.


在接下来的几十年里,我们需要在农作物产量上有大的提升。然而,植物育种目前限制在对于数量性状的改良,而这通常依赖基因调控区罕见的自然突变。本文展示了对启动子区域进行CRISPR/Cas9基因组编辑可以产生多种顺式调控等位基因,可进一步用于数量变异的育种研究。作者设计了一个简单的遗传方案,用来挖掘Cas9在杂合功能缺失突变体中活性的跨代遗传,以快速评估影响番茄三大产量性状(果实大小、花序分枝和株型)的基因启动子区变异对于表型的影响。本文的方法可用于直接选择,并在无转基因的植株中固定新的等位基因,同时对产量组分进行精细调控。除了用于增强农艺性状多样性之外,本文的研究还可为深入研究基因调控改变与数量性状控制之间的复杂关系奠定基础。


通讯:Zachary B. Lippman (https://www.cshl.edu/Faculty/Zachary-Lippman.html)


研究方向:决定开花时间、部位及数量的基因网络。


doi: http://dx.doi.org/10.1016/j.cell.2017.08.030


Journal: Cell
Published data: September 14, 2017.

P.S. 欢迎关注微信公众号:微信号Plant_Frontiers


https://m.sciencenet.cn/blog-3158122-1081475.html

上一篇:Rosalind 2 - Variables and Some Arithmetic
下一篇:Rosalind 3 - Strings and Lists

0

该博文允许注册用户评论 请点击登录 评论 (0 个评论)

数据加载中...
扫一扫,分享此博文

Archiver|手机版|科学网 ( 京ICP备07017567号-12 )

GMT+8, 2024-3-29 18:39

Powered by ScienceNet.cn

Copyright © 2007- 中国科学报社

返回顶部