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Plant Physiology:棉花中长、短绒纤维的转录组研究

已有 3843 次阅读 2017-10-23 08:28 |个人分类:每日摘要|系统分类:论文交流|关键词:学者

Transcriptomic repertoires depict the initiation of lint and fuzz fibres in cotton (Gossypium hirsutumL.)


First author:Haiyan Hu; Affiliations: Huazhong Agricultural University (华中农业大学): Wuhan, China

Corresponding author: Xianlong Zhang (张献龙)


Cotton fibre is an important natural fibre for the textile industry (纺织业). The number of fibre initials determines the lint (棉纤维) percentage, which is an important factor for cotton fibre yield. Although fibre development has been described by transcriptomic analysis, the mechanism by which the long noncoding RNA manipulates the initiation of lint (长绒纤维) and fuzz fibers (短绒纤维) remains unknown. In this study, three lines with different lint percentages were developed by crossing Xu142 with its fibreless mutant Xu142 fl. We collected the epidermal cells (表皮细胞) from the ovules (胚珠) with attached fibres at 0 and 5 days post anthesis (DPA; 花后) from Xu142, the fibreless mutant Xu142 fl and the three lint percent diversified lines for deep transcriptome sequencing. A total of 2641 novel genes, 35 802 long noncoding RNAs (lncRNAs) and 2262 circular RNAs (circRNAs) were identified, of which 645 lncRNAs were preferentially expressed in the fibreless mutant Xu142 fl and 651 lncRNAs were preferentially expressed in the fibre-attached lines. We demonstrated the functional roles of the three lncRNAs in fibre development via a virus-induced gene silencing (VIGS) system. Our results showed that silencing XLOC_545639 and XLOC_039050 in Xu142 fl increased the number of fibre initials on the ovules, but silencing XLOC_079089 in Xu142 resulted in a short fibre phenotype. This study established the transcriptomic repertoires in cotton fibre initiation and provided evidence for the potential functions of lncRNAs in fibre development.


棉纤维是纺织业最为重要的天然纤维。纤维起始的数量决定了纤维含量,这是棉纤维产业中最重要的因素。尽管纤维发育已经有了详尽的转录组分析,但长链非编码RNA(lncRNA)调控长、短绒纤维起始的机制还不清楚。本文中,作者通过Xu142和其无棉纤维突变体fl杂交育成三个不同纤维含量的株系。作者收集了Xu142、无棉纤维突变体fl和三个不同纤维含量棉花株系中花后0天和10天带有纤维的胚珠中的表皮细胞用于转录组测序。作者共鉴定了2641个新基因、35 802个lncRNA和2262个环状RNA(circRNA),其中645个lncRNA优先在无棉纤维突变体fl中表达,651个lncRNA优先在带棉纤维株系中表达。作者通过病毒诱导沉默(VIGS)系统研究了三个lncRNA在纤维发育中的作用。研究结果显示在Xu142 fl突变体中沉默XLOC_545639和XLOC_039050两个基因会增加胚珠中纤维起始的数量,但在Xu142中沉默XLOC_079089基因导致纤维变短。本文的研究进行了棉花纤维起始的转录组分析,为lncRNA在纤维发育中的潜在功能提供了新的见解。


通讯:张献龙 (http://cpst.hzau.edu.cn/szdw/jsml/js/201702/t20170221_100933.htm)


个人简介:1980.09-1984.06,华中农业大学,作物遗传育种,获学士学位;1984.09-1987.06,华中农业大学,作物遗传育种获硕士学位;1987.09-1990.06,华中农业大学,作物遗传育种获博士学位;1990.07-1992.12 华中农业大学,讲师;1993.01-1996.12,华中农业大学,副教授;1997.01-2002.05,华中农业大学,教授;2002.06-2008.02,华中农业大学,植物科技学院院长,教授;2008.03-至今,华中农业大学,副校长,教授;1993.10-1994.10,加拿大曼尼托巴大学农学院植物科学技术系访问学者;1998.11-1999.04,美国阿拉巴马农工大学植物科学系访问学者;2001.11-2002.02,英国杜伦大学生物系访问学者。


研究方向棉花生物技术及育种应用研究


doi: https://doi.org/10.1104/pp.17.01010


Journal: Plant Physiology
First Published data: October 19, 2017.


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