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Plant Physiology:GA通过独立于DELLA的信号通路提高胞内钙离子

已有 4054 次阅读 2017-10-26 08:34 |个人分类:每日摘要|系统分类:论文交流|关键词:学者

Gibberellin induces an increase in cytosolic Ca2+ via a DELLA-independent signaling pathway


First author:Kanako Okada; Affiliations: Hiroshima University (广岛大学): Higashi-Hiroshima, Japan

Corresponding author: Yohsuke Takahashi


DELLA proteins play a central role in gibberellin (GA) signaling. GA triggers DELLA degradation (降解) via the ubiquitin-proteasome pathway (泛素-蛋白酶体通路), thereby promoting plant growth. An increase in cytosolic (细胞质的) Ca2+ ([Ca2+]cyt) was previously observed after several hours GA application. Recent studies also suggest the existence of a DELLA-independent GA response. However, the effect of DELLA on the GA-induced increase in [Ca2+]cyt remains unknown. This study re-examined the effects of GAs on [Ca2+]cyt using the Ca2+ sensor protein aequorin (发光蛋白质). [Ca2+]cyt increased within a few minutes of GA treatment, even in transgenic plants expressing a mutated degradation-resistant version of RGA and in della pentuple mutant plants. In addition, it was also revealed that Ca2+ is not involved in DELLA degradation. These results suggest that the GA-induced increase in [Ca2+]cyt occurs via a DELLA-independent pathway, providing important information on the GA signaling network.


DELLA蛋白在赤霉素GA信号转导中扮演着重要作用。GA会通过泛素-蛋白酶体通路诱导DELLA蛋白的降解,因此促进植物的生长。先前在GA处理数小时后观察到胞内钙离子溶度会上升。最近的研究表明存在独立于DELLA的GA响应。然而,DELLA对于GA诱导的胞内钙离子溶度增加的影响还不清楚。本文利用钙离子感应发光蛋白研究GA对于胞内钙离子溶度的影响。胞内钙离子溶度在GA处理后的几分钟就会增加,这种现象在突变了的RGA降解抗性的转基因植株中和della突变体植株中同样存在。另外,试验同样表明Ca2+并不参与DELLA的降解。这些结果显示GA诱导的胞内钙离子溶度增加是通过独立于DELLA通路,这为GA信号通路网络提供了重要的信息。


doi: https://doi.org/10.1104/pp.17.01433


Journal: Plant Physiology
First Published data: October 24, 2017.

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