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Nature Plants:F-box蛋白UFO通过控制LFY的结合元件,调控植物花发育

已有 1833 次阅读 2023-2-9 19:57 |个人分类:每日摘要|系统分类:论文交流

The F-box protein UFO controls flower development by redirecting the master transcription factor LEAFY to new cis-elements

第一作者Philippe Rieu

第一单位格勒诺布尔-阿尔卑斯大学

通讯作者François Parcy


 ABSTRACT 

背景回顾:In angiosperms, flower development requires the combined action of the transcription factor LEAFY (LFY) and the ubiquitin ligase adaptor F-box protein, UNUSUAL FLORAL ORGANS (UFO), but the molecular mechanism underlying this synergy has remained unknown. 


结果1-UFO泛素化功能不必需Here we show in transient assays and stable transgenic plants that the connection to ubiquitination pathways suggested by the UFO F-box domain is mostly dispensable. 


结果2-UFO-LFY转录激活复合体On the basis of biochemical and genome-wide studies, we establish that UFO instead acts by forming an active transcriptional complex with LFY at newly discovered regulatory elements. 


结果3-UFO招募LFY至新顺式元件上Structural characterization of the LFYUFODNA complex by cryo-electron microscopy further demonstrates that UFO performs this function by directly interacting with both LFY and DNA. 


结论Finally, we propose that this complex might have a deep evolutionary origin, largely predating flowering plants. This work reveals a unique mechanism of an F-box protein directly modulating the DNA binding specificity of a master transcription factor.

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 摘 要 

在被子植物中,花发育需要转录因子LEAFY和泛素连接酶接头F-box蛋白UFO的组合作用,但是这两个蛋白之间协调作用的分子机制还不清楚。本文中,作者通过瞬时转化和稳定转化发现,UFO参与花发育调控并不依赖于其F-box结构域所介导的泛素化途径。基于生化和基因组范围的研究,作者发现UFO通过与LFY形成一个激活转录复合物,结合到新发现的调控元件上。通过冷冻电镜对LFY–UFO–DNA复合体的结构解析,作者发现UFO通过同时直接与LFY和DNA结合,从而执行该功能。最终,作者提出该复合物可能具有一个比较原始的演化起源,可能要早于有花植物出现的时间。本文的研究结果揭示了一个F-box蛋白直接调控关键转录因子的DNA结合特异性的独特机制。




** François Parcy **


个人简介:

1992-1995年,CRNS Délégation Ile-de-France Sud,博士。


研究方向

LEAFY和ARFs在花发育过程中的作用。


doi: https://doi.org/10.1038/s41477-022-01336-2


Journal: Nature Plants

Published date: February 02, 2023


Cite:
Philippe Rieu, Laura Turchi, Emmanuel Thévenon, Eleftherios Zarkadas, Max Nanao, Hicham Chahtane, Gabrielle Tichtinsky, Jérémy Lucas, Romain Blanc-Mathieu, Chloe Zubieta, Guy Schoehn, François Parcy. The F-box protein UFO controls flower development by redirecting the master transcription factor LEAFY to new cis-elements. Nat. Plants (2023). https://doi.org/10.1038/s41477-022-01336-2



https://m.sciencenet.cn/blog-3158122-1375582.html

上一篇:Plant Physiology:野草莓中4个LFY基因拷贝在花模式建成和叶发育中的作用
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