氢分子医学分享 http://blog.sciencenet.cn/u/孙学军 对氢气生物学效应感兴趣者。可合作研究:sunxjk@hotmail.com 微信 hydrogen_thinker

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高压氧预适应诱导心脏新血管生长

已有 6399 次阅读 2009-2-7 11:41 |个人分类:高压氧|系统分类:科研笔记|关键词:学者| 论文, 高压氧, 高压氧, 缺血, 高压氧, 缺血

证明高压氧能诱导心脏新血管生长

05年的时候,国际上开展这个方面的实验室我们都比较熟悉:美国、日本、德国、英国和中国的四军大。从文章档次和数量上看,四军大的工作最出色.英国在心脏保护方面最系统.日本的工作最早,后来的工作都比较缓慢.而我们现在的进展最快.现在已经在数量上领先所有的实验室.但质量与四军大还有很大差距.需要继续深入研究.

这些实验室的特点是方向比较集中,但重点都不在高压氧这个方向上,都是在各自的领域涉及到高压氧。而我们可以不受这些限制。我们了解到英国已经有临床研究:在心脏手术前进行高压氧暴露,可有效预防手术造成的心肌缺血损伤,这个研究非常重要,说明这个手段确实能在临床上使用。这也是唯一的临床证据。

 

给这个领域的研究带来很好的期望。我们也希望能做一些动物实验证明对心脏的作用。正好我们了解到本校有个老师在心肌缺血方面研究基础很好,于是我派一个学生学习,结果很快,我们证证明明这个模型效果特别好。这个阶段,我们做了一些干细胞方面探讨,主要是我们发现高压氧能诱导新生血管生成。结果我们只用BRDU方面不能确定细胞的类型,审稿专家给我们提出这个问题。后来美国的张教授建议我们把这个方面的内容去掉,结果杂志同意发表了。这个文章的启发是,任何实验,要么别做,要做就全面,别留下太多漏洞。否则会浪费时间。当然这个想法十分功利。不提倡

 Exp Biol Med (Maywood). 2008 Nov;233(11):1448-53. Epub 2008 Aug 14.Click here to read Links

 

 

Hyperbaric oxygen preconditioning alleviates myocardial ischemic injury in rats.

Department of Diving Medicine, Second Military Medical University, Shanghai 200433, People's Republic of China.

It has been shown that after ischemia-reperfusion, application of hyperbaric oxygen (HBO) reduces cardiac injury. In this study we tested the hypothesis that HBO preconditioning reduces injury to the ischemic myocardium. One hundred and eight adult male Sprague-Dawley rats (250-280 g) were randomly divided into four groups: normoxia + sham surgery (CS), normoxia + permanent occlusion of the left anterior descending (LAD) coronary artery (CMI), HBO preconditioning + sham surgery (HS), and HBO preconditioning + permanent LAD occlusion (HMI). Rats receiving HBO preconditioning were intermittently exposed to 100% O(2) at 2.5 atmosphere absolute (ATA) for 60 min, twice daily for 2 days followed by 12 hrs of recovery in room air prior to the myocardial ischemic insult induced by LAD ligation. Rats in the normoxia group were time-matched with the HBO group and maintained under normoxic conditions prior to LAD occlusion. At 3 and 7 days after LAD occlusion, heart function parameters were measured by inserting a catheter into the left ventricle, infarct size was calculated using the method of TTC staining, myocardial capillary density was determined by immunohistochemical staining with a monoclonal anti-CD(31)/PECAM-1 antibody, and VEGF protein level was determined by Western blot analysis. At 3 days after LAD ligation, the infarct size of the HMI group was significantly smaller than that of the CMI group (26 +/- 2.5% vs. 38 +/- 3%, P < 0.05). The heart function parameters including left ventricular systolic pressure (LVSP), +dP/dt(max) and -dP/dt(max) were significantly improved in the HMI group compared to the CMI group at 3 and 7 days after LAD occlusion. Capillary density and VEGF protein levels were significantly increased in the ischemic myocardium pre-exposed to HBO. We conclude that HBO preconditioning alleviates myocardial ischemia in rat model.

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