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- JANEWANGJK55
- http://jac.oxfordjournals.org/content/early/2014/04/11/jac.dku098.abstract?maxtoshow=&HITS=10&hits=1&RESULTFORMAT=&andorexacttitle=and&andorexacttitleabs=and&fulltext=ESBL&andorexactfulltext=and&searchid=1&usestrictdates=yes&resourcetype=HWCIT&ct
- JANEWANGJK55
- http://www.nature.com/naturejobs/science/jobs/325006-phd-student-for-the-project-plasmid-biology
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PhD student for the project “plasmid biology” : Flevoland, Netherlands
- JANEWANGJK55
- PhD student for the project “plasmid biology” : Flevoland, Netherlands
We are looking for
a PhD student for the project “plasmid biology”.
Plasmid mediated ESBLs or AmpC-beta-lactamases have emerged and spread in humans and animals in the last decade. Both in animals and humans predominant plasmid/gene combinations determine the epidemiology. The dominant plasmids are genetically closely related. These plasmids associated with a specific ESBL/AmpC-variant, are considered to be epidemic. Their success will be related to plasmid-and host encoded factors. The aim of the project is to identify the genetics and mechanisms that determine the evolutionary success of epidemic IncI1-plasmid-ESBL combinations.
To study the behaviour of these plasmids, conjugation studies will be conducted for a representative selection of plasmids that belong to various clonal complexes (CC’s). The transfer frequencies and incompatibility of these plasmids to recipients with known chromosomal background such as K12/DH10ß will be determined.
Plasmids and hosts will be genetically modified to confirm the effects measured during in vitro conjugation and plasmid replacement, and also to identify and characterize functional regions involved in plasmid transfer and maintenance. Genes encoding for entry exclusion proteins, partitioning, colicin production and addiction factors previously found to be associated with plasmids of various CC’s will be targeted in these studies.
Sequence analysis
In the first phase, a selection of isolated epidemic and control plasmids will be sequenced to determine the genetic characteristics of the plasmids. All purified plasmids will be sequenced by a new generation-sequencing approach (MiSeq) in multiplex pools of 15 – 30 plasmids. The coverage will be fully sufficient to identify the genetic characteristics of these plasmids and genetic differences.
In the second phase, to identify the genetic association between bacterial host- cells and their plasmids, selected isolates harbouring these plasmids will be sequenced using a metagenomics approach. Selection will be such that phenotypically and genetically interesting representative isolates are included. The sequences will be analysed in comparison with reference genomic data of isolates from GeneBank/EMBL and plasmids from phase one of the present study. The genetic association between the plasmids and their hosts on a strain and population level will be studied.
We ask
an enthusiastic and motivated candidate with an MSc degree in biomedical sciences (molecular biology or biochemistry) and an interest in the molecular epidemiology of antimicrobial resistance. Ideally the candidate combines an interest and expertise in laboratory analyses and bioinformatics. We aim for a candidate who can demonstrate an attitude for academic research as evidenced by for example: publications; prior engagements in collaborative research. Furthermore excellent written and verbal communication skills in English are requested. We expect a keenness to actively and independently perform laboratory work. The project will be conducted in collaboration with dr. Axel Cloeckaert and dr. Benoit Doublet from the INRA in Tours-Nouzilly (F) of the Department Plasticité Génomique, Biodiversité, Antibiorésistance (PGBA).
We offer
a stimulating work environment and unique integration in a team of experts to develop on-the-job academic skills. The candidate will be appointed at the Department of Infectious Diseases and immunology of the faculty of Veterinary Medicine of Utrecht University, but the project will be conducted at the Central Veterinary Institute, part of Wageningen UR (CVI) in Lelystad according to the UU regulations for PhD students. Part of the work will be conducted at INRA in Tours.
The appointment will be for a period of one year with an extension of three years after positive evaluation of capabilities and compatibility. Ultimately the appointment must lead to the completion of a PhD thesis after 4 years. During your appointment you will be supervised by Prof. Dr. Dik J. Mevius (promotor), dr. Hilde Smith and dr. Alex Bossers (co-promotors).
Wageningen UR (University & Research centre)
Delivering a substantial contribution to the quality of life. That's our focus each and every day. Within our domain, healthy food and living environment, we search for answers to issues affecting society such as sustainable food production, climate change and alternative energy. Of course, we don’t do this alone. Every day, 6,500 people work on ‘the quality of life’, turning ideas into reality, on a global scale.
Could you be one of these people? We give you the space you need.
For further information about working at Wageningen UR, take a look at www.jobsat.wur.nl.
Acquisition regarding this vacancy is not appreciated.
Don't forget to mention Naturejobs when applying.
- JANEWANGJK55
- Job details
Employer
Wageningen UR
Website
http://www.werkenbij.wur.nl/uk
Location
Lelystad, Flevoland, The Netherlands, Flevoland, Netherlands
Posted
12 months ago
Expires
May 26, 2014
Job type
PhD Studentship
Salary
Unspecified
Qualifications
Unspecified
Employment type
Unspecified
Job hours
Unspecified
