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Plant Biotechnology Journal∣华南农业大学利用CRISPR免疫系...

已有 2090 次阅读 2018-1-14 22:00 |系统分类:论文交流|关键词:学者

2018年1月11日Plant Biotechnology Journal在线文章,题目为Establishing RNA virus resistance in plants by harnessing CRISPR immune system


本文为华南农业大学周国辉团队完成。文章首次报道了利用CRISPR介导的免疫系统建立的植物RNA病毒抗性研究。


摘要:

最近,CRISPR-Cas系统已被用于产生抗DNA病毒感染的植物。然而,在植物中还没有使用CRISPR-Cas系统直接靶向RNA病毒基因组来控制病毒的方法。在这里,我们重新编程了来自Francisella novicida 的CRISPR-Cas9系统,赋予其在本氏烟草和拟南芥中抗RNA病毒的分子免疫力。在植物中表达对黄瓜花叶病毒(CMV)或烟草花叶病毒(TMV)特异性的FnCas9和sgRNA,表现出显著的减弱的病毒感染症状和减少病毒RNA的积累。此外,在转基因病毒靶向的植物中,抗性是可遗传的,后代表现出显著较少的病毒积累。这些数据揭示了CRISPR/Cas9系统可用于生产对RNA病毒具有稳定抗性的植物,从而扩大了这种技术在农业领域病毒防治的应用。


Abstract:

Recently, CRISPR-Cas (clustered, regularly interspaced short palindromic repeats-CRISPR associated proteins) system has been used to produce plants resistant to DNA virus infections. However, there is no RNA virus control method in plants that uses CRISPR-Cas system to target the viral genome directly. Here we reprogrammed the CRISPR-Cas9 system from Francisella novicida to confer molecular immunity against RNA viruses in Nicotiana benthamiana and Arabidopsis plants. Plants expressing FnCas9 and sgRNA specific for the cucumber mosaic virus (CMV) or tobacco mosaic virus (TMV) exhibited significantly attenuated virus infection symptoms and reduced viral RNA accumulation. Furthermore, in the transgenic virus-targeting plants, the resistance was inheritable and the progenies showed significantly less virus accumulation. These data reveals that the CRISPR/Cas9 system can be used to produce plant that stable resistant to RNA viruses, thereby broadening the use of such technology for virus control in agricultural field.








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