博文
能合成多肽的人造小分子机器
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核糖体按照信使RNA指定的氨基酸序列来构建蛋白质。在这里，研究人员设计、合成了一个小分子机器，该机器沿着的分子链按照序列特异性方式进行多肽合成。该小分子机器的化学结构是基于一个轮烷，分子环拧到一个分子桥。环带有硫醇盐基团，可以反复按照特定顺序删除氨基酸，并将它们运送到的多肽 生长延长位点。实验结果表明大约有1018的小分子机器在并行进行合成；这一过程会产生毫克量级多肽。串联质谱实验确认只产生了单一多肽序列。
英文全文见：
http://www.sciencemag.org/content/339/6116/189.full
Sequence-Specific Peptide Synthesis by an Artificial Small-Molecule Machine
The ribosome builds proteins by joining together amino acids in an order determined by messenger RNA. Here, we report on the design, synthesis, and operation of an artificial small-molecule machine that travels along a molecular strand, picking up amino acids that block its path, to synthesize a peptide in a sequence-specific manner. The chemical structure is based on a rotaxane, a molecular ring threaded onto a molecular axle. The ring carries a thiolate group that iteratively removes amino acids in order from the strand and transfers them to a peptide-elongation site through native chemical ligation. The synthesis is demonstrated with ~1018 molecular machines acting in parallel; this process generates milligram quantities of a peptide with a single sequence confirmed by tandem mass spectrometry.
https://m.sciencenet.cn/blog-637163-651914.html
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