(Text adopted from www.protocol-online.org)

I often wondered, why some companies supply their PCR Ingredients with MgCl2 and some with MgSO4. Is there ANY difference between them ?

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Not much difference, won't affect normal PCR, where the key is the amount of available Mg2+. I think that SO42- might be useeful in methylation PCR, but I am not sure.

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Recipes using Tris-HCl tend to use MgCl2, while recipes employing Tris-SO4/(NH4)2SO4 tend to use MgSO4.

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