NIH Roadmap 计划定位引用 Science 的评述 “The NIH Roadmap is a set of bold initiatives aimed ataccelerating medical research. These initiatives will address challenges that no single NIH institute could tackle alone, but the agency as a whole must undertake. The Roadmap identifies the most compelling opportunities in three arenas: new pathways to discovery, research teams of the future, and reengineering the clinical research enterprise. ”(Zerhouni E. Medicine. The NIH Roadmap. Science. 2003 Oct3;302(5642):63-72.). 2014 年 NIH Roadmap 引入新的领域值得我们关注，将代表医学前沿研究的发展方向： 1. Glycoscience （糖科学） is the study of howthe addition of sugar modifications to proteins change the way the proteins function in important ways. The complexity of carbohydrate chemistry makes theanalysis of these sugar modifications inaccessible to most biomedicalresearchers. The Glycoscience program will develop methodologies and resourcesto make the study of sugar modifications more accessible to the broadbiomedical research community. 2. The 4D Nucleome program （ 4D 核小体） will develop technologies to enable the study of how DNA is arranged within cells in spaceand time (the fourth dimension) and how this affects cellular function inhealth and disease. Recent scientific advances, coupled with technological breakthroughs in tools and methods, provide the opportunity to catalyze thisemerging field of research. 4D nucleome science aims to understand theprinciples behind the organization of the nucleus in space and time, the rolethat the arrangement of DNA plays in gene expression and cellular function, andhow changes in nuclear organization affect health and disease. 3. The Stimulating Peripheral Activity to Relieve Conditions (SPARC) program （外周神经刺激治疗） will develop high resolution neural circuit maps and next generation neural modulation devices –implants that can stimulate nerves – and will demonstrate the use of these tools in the development of new therapeutic strategies. All organs arestimulated by nerves, which send signals that affect the organ’s function.Modulation of nerve signals to control organ function has therefore beenrecognized as a potentially powerful way to treat many diseases and conditions,such as hypertension, heart failure, gastrointestinal disorders, type II diabetes, and inflammatory disorders. 信息来源： http://commonfund.nih.gov/ 附： NIH Roadmap CommonFund Programs Common Fund Programs CURRENT PROGRAMS 中文对照 4D Nucleome 四维核小体 Big Data to Knowledge 从大数据到知识 Bioinformatics and Computational Biology 生物信息学与计算机生物学 Bridging Interventional Development Gaps (BrIDGs) 缩小干预发展差距计划 Building Blocks, Biological Pathways and Networks 创建技术模块、生物学通路及网络 Enhancing the Diversity of the NIH-Funded Workforce 加强 NIH 资金提供、人员配置的多样性 Epigenomics 表观基因组学 Extracellular RNA Communication 细胞外 RNA 通信机制 Genotype-Tissue Expression (GTEx) 基因型组织的表达 Global Health 全球卫生 Glycoscience 糖科学 Gulf Oil Spill 海湾石油泄漏 HCS Research Collaboratory 医疗系统研究合作实验室 Health Economics 卫生经济学 HIGH-RISK RESEARCH: 高风险性研究： NIH Director's Early Independence Award (EIA) NIH 早期独立奖 NIH Director's New Innovator Award NIH 新创新者奖 NIH Director's Pioneer Award NIH 先锋奖 NIH Director's Transformative Research Awards NIH 研究转化奖 Human Microbiome Project (HMP) 人类微生物组计划 Illuminating the Druggable Genome 药用基因组计划 Knock out Mouse Phenotyping (KOMP2) 小鼠表现型分析 Library of Integrated Network-Based Cellular Signatures (LINCS) 基于综合网络的细胞特征库 Metabolomics 代谢组学 Molecular Libraries and Imaging 分子库和成像 Nanomedicine 纳米医学 NIH Center for Regenerative Medicine (NIH CRM) NIH 再生医学中心 NIH Medical Research Scholars Program 医学研究学者计划 PROMIS: Patient-Reported Outcomes Measurement Information System 患者结局报告计量信息系统 Protein Capture Reagents 蛋白质分离跟踪研究 Regulatory Science 法规科学 Science of Behavior Change 行为变化科学 Single Cell Analysis 单细胞分析 Stimulating Peripheral Activity to Relieve Conditions (SPARC) 外周神经刺激疗法 Strengthening the Biomedical Research Workforce 加强生物医学研究队伍 Structural Biology 结构生物学 Undiagnosed Diseases 未明机理疾病的研究 EXPORTED PROGRAMS Clinical Research Policy Analysis and Coordination (CRPac) 临床研究政策分析与协调 Clinical and Translational Science Awards (CTSAs) 临床和转化科学奖 Interdisciplinary Research (IR) 跨学科研究 National Electronics Clinical Trials and Research (NECTAR) 国家电子临床试验研究 （文 / 严舒 贾晓峰）
The ENCODE Dataset Here’s a brief summary of the dataset described in the main paper: Element Approach Description Genes and RNAs Annotation Automated and manual annotation to produce the GENCODE reference gene set (v7) of protein-coding genes, pseudogenes, and non-coding RNAs. Transcribed regions RNA-seq An extensive RNA expression catalogue from different cell lines and multiple subcellular fractions. Transcription start sites CAGE-seq 5′ cap-targeted RNA isolation and sequencing identified 62,403 high confidence TSSs. Full-length RNAs RNA-PET Simultaneous capture of RNAs with both a 5′ methyl cap and a poly-A tail Protein-coding regions Mass spec Peptide sequences identified by mass spectrometry in two cell lines (K562 and GM12878) TF binding sites CHiP-Seq Mapping binding locations of 119 transcription factors and several RNA polymerase components in 72 cell types. Open chromatin DNase-Seq footprinting, FAIRE Sequencing of DNase I hypersensitive sites in (125 cell types), FAIRE analysis of reduced nucleosome crosslinking (25 cell types), and genomic DNase I footprinting (41 cell types). Histone modification CHiP-Seq Assays for up to 12 histone modifications and variants in 46 cell types DNA methylation RRBS Reduced representation bisulfite sequencing to profile 1.2m CpGs in 82 cell lines and tissues Chromosome interactions 5C and ChiA_PET Chromosome conformation capture carbon-copy (5C) in a targeted 1% (4 cell types). Genome-wide chromatin interaction analysis with paired tag sequencing (ChIA-PET) of PolII CHiP in 5 cell types. together with roadMap project, we could do integrative analysis to obtain a landscape of regulatory mechanism across the cell Lines, which are generally considered as open Chromatin region.
标签: Roadmap
