Procedia Environmental Sciences 23 ( 2015 ) 407 – 411 岩藻多糖抗胃溃疡的生物活性评价研究 岩藻多糖日报 原标题:岩藻多糖作为小鼠抗胃溃疡的生物活性评价研究 ① 本研究岩藻多糖提取物的组成为:含水量3.11%,尿酸556ppm,硫酸盐0.12ppm,总碳水化合物1648ppm。 ② 根据体内试验结果显示,使用浓度为100ppm的岩藻多糖具有防止胃溃疡的作用。岩藻糖能使胃粘膜分层,所以不会损伤胃组织的内层。 ③ 胃粘液是一种糖蛋白,有两种作用:一是润滑食物团,以促进其在胃内运动。二是在胃腔内上皮细胞上形成保护层。这一保护层是一种防御机制,胃可以抵抗自身的赖氨酸蛋白酶的消化,并通过碳酸氢盐从下面的粘膜分泌到表层。 ④ 本研究结果表明,根据100 ppm岩藻多糖对小鼠的组织病理学检查,给予阿司匹林400 mg/kg后,岩藻多糖可有效抑制胃溃疡刺激。岩藻多糖与胃粘膜粘液层的增加有关。 ⑤ 关键词:岩藻多糖,诱发胃溃疡,阿司匹林,生物活性; 延伸阅读: Evaluation of fucoidan bioactivity as antigastric ulcers in mice ABSTRACT: Fucoidan is a polysaccharide compounds containing sulfate group. Fucoidan is found in brown seaweed. In this study,we assess fucoidan activity extracted from brown seaweed Sargassum crassifolium origin from Binuangeun, Banten. Fucoidan extract was tested in mice in vivo. Observations were carried out during 16 days the control (without fucoidan) and fucoidan treatment. Fucoidan were given in various concentration of 100, 200, 300, 400 ppm. On the 14th day, aspirin was given to mice with pre-treated fucoidan 400 ppm as gastric ulcer induction. The fucoidan extracts compositions showed: water content 3.11%, uronic acid 556 ppm, 0.12 ppm sulfate and 1648 ppm total carbohydrate. Results from histopathology assay in mice tissue stomach showed that 100 ppm of fucoidan can inhibit gastric ulcers caused by 400 ppm aspirin irritation. Fucoidan was associated with an increase in the mucus layer in the gastric mucosa. KEYWORDS: fucoidan, induced-gastric ulcer, irritation, aspirin, bioactivity All Authors: Ellya Sinurat , Dan Rosmawaty
国际护胃日”又称“国际养胃日”,2006年的4月9日被国际自然医学和养生工程研究会、世界健康管理联盟定为第一个“国际养胃日”,今年4月9日是第15个“国际护胃日”。 脾胃乃后天之本。中国是“胃病大国”,十人九胃,我国胃癌的发病率目前在所有恶性肿瘤中占第二位,死亡率高居第三位。更为严峻的是,目前我国每年胃癌新发病例超过42万例,占到全球新发病例总数的42%,居全球首位。大量研究发现,胃病高发的 罪魁祸首,便是 幽门螺旋杆菌(下称:HP)感染 。 解决胃病,不能忽视幽门螺杆菌 大多数胃病都是由于幽门螺杆菌感染。如果已经有幽门螺杆菌感染的迹象:消化不良、饭后打嗝、反酸、胃痛、口臭,那么就要尽快清除干净,不仅是因为不清除,胃病就无法好,还因为它能将人类患上胃癌的几率提高4-6倍。幽门螺杆菌会通过 口口传播(唾液)、粪口传播 ,在人群中的感染率很高,中国人的幽门螺杆菌感染率保守估计在60%。 解决幽门螺杆菌,食疗很重要 胃病三分治七分养,所以如果出现幽门螺杆菌超标、胃部不适的话,在日常生活中一定要重视胃部的养护。不管是早饭、午饭,还是晚饭,都要做到只吃七八分饱。糯米类的食物比如粽子、青团、糯米饭等黏糊糊的食物尽量少吃,酒精、辛辣刺激食物要尽量避免。作息方面,一定要减少或 避免熬夜 ,因为熬夜会干扰胃黏膜的修复。此外,可适当多食用猴菇、秋葵、山药等 黏滑食物 ,建议 每日补充岩藻多糖 (海带表面黏液)。 岩藻多糖:靶向 吸附 清除HP、修护胃黏膜 岩藻多糖又名褐藻糖胶、褐藻多糖硫酸酯,提取自 葛洪《肘后备急方》 收录的昆布等褐藻表面黏液,富含L-岩藻糖和有机硫酸根,是海洋中独有的天然功能型多糖(可分为食品级、药用级)。以昆布、裙带菜等褐藻为食物的海参、鲍鱼,通过食物链其体内会富集少量的岩藻多糖,这些岩藻多糖及其衍生物成为 海参、鲍鱼 等养生保健价值的重要组成部分( 杨玉红. 海参岩藻聚糖硫酸酯抗肿瘤活性及作用机制的研究 . 中国海洋大学, 2012 )。大量研究发现,岩藻多糖对清除幽门螺杆菌效果独特,且能修护胃黏膜,是 安全清幽的新选择 (目前,针对幽门螺杆菌的四联方等抗生素疗法耐药性越发严重、副作用大)。 2011年 ,《International Immunopharmacology》报道岩藻多糖可以保护阿司匹林引发的胃黏膜损伤。 2012年 ,《Food Chemistry》报道岩藻多糖可以保护酒精性胃损伤 2014年 , 2014年,韩国Chungbuk National University大学Yun-Bae Kim研究团队在《Lab Anim Res》发表研究显示,岩藻多糖浓度为100μg/mL时能够完全抑制幽门螺旋杆菌的增殖。 2015年 ,发表在《癌症治疗》(Journal of Cancer Therapy)期刊上的一项日本临床研究强调了岩藻多糖应该被作为胃癌患者的膳食补充剂,以减少患者化疗副作用,延长生命期。 2019年 ,海藻活性物质国家重点实验室与青岛大学附属医院合作进行的临床研究表明,岩藻多糖配合四联疗法能够增强临床幽门螺杆菌根除率。 (1)Shibata H , KimuraTakagi I , Nagaoka M , Hashimoto S , et al. Inhibitory effect of Cladosiphon fucoidan on the adhesion of Helicobacter pylori to human gastric cells . Journal of Nutritional Science and Vitaminology,1999:45,325-336 (2)Tae-Su Kim, Ehn-Kyoung Choi, Jihyun Kim, Kyungha Shin, Sung-Pyo Lee, Youngjin Choi,et al. Anti- Helicobacter pylori activities of FEMY-R7 composed of fucoidan and evening primrose extract in mice and humans. Lab Anim Res,2014:30(3),131-135 (3)Jingmei Cai,Tae-Su Kim,Ja Young Jang,Jihyun Kim,Kyungha Shin, Sung-Pyo Lee,et al. In vitro and in vivo anti- Helicobacter pyl ori activities of FEMY-R7 composed of fucoidan and evening primrose extract . Lab Anim Res, 2014: 30(1), 28-34 (4) 阮研硕 , 赵江燕 , 李艳梅 , et al. 岩藻多糖对急性酒精性 胃黏膜损伤的保护作用 . 中国食品学报 , 2015, 15(1):19-24 (5) Raghavendran H R B , Srinivasan P , Rekha S . Immunomodulatory activity of fucoidan against aspirin-induced gastric mucosal damage in rats . Internationa l Immunopharmacology, 2011 , 11(2):157-163 ; 岩藻多糖对阿司匹林致大鼠胃粘膜损伤的作用
我们实验室今年被接受发表的第7篇SCI论文由张爽完成。 Bidirectional crosstalk between stress-induced gastric ulcer and depression under chronic stress Shuang Zhang 1,2 , Zhiwei Xu 1 , Yan Gao 1 , Yonghong Wu 1 , Zhihui Li 1 , Haifeng Liu 2, *, Chenggang Zhang 1,3 * 1 Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Cognitive and Mental Health Research Center, Beijing 100850, China 2 Department of Gastroenterology, the General Hospital of Chinese People’s Armed Police Forces, Beijing 100039, China 3 School of Life Science, Anhui Medical University, Anhui, 230032, China Running title: Crosstalk between gastric u lcer and depression Abstract Stress contributes to a variety of diseases and disorders such as depression and peptic ulcer. The present study aimed to investigate the correlation between stress ulcer and depression in pathogenesis and treatment by using chronic stress depression (CSD), chronic psychological stress ulcer (CPSU) and water immersion restrain stress models in rats. Our data showed that the ulcer index of the animals after CSD exposure was significantly higher than that of controls. Depression-like behaviors were observed in rat after CPSU exposure. Fluoxetine hydrochloride significantly reduced the ulcer index of rats exposed to CPSU stress, while ranitidine inhibited depression-like behavior of the animals in CSD group. The ulcer index of rats administered with mifepristone after CPSU stress was markedly reduced compared to CPSU group, although there was no significant difference in the depression-like behavior between mifepristone-treated CSD group and naive controls. We also found that the rats exposed to CPSU or CSD stress displayed a lower level of corticosterone than naive controls, however, the acute stress (AS) group showed an opposite result. Additionally, in order to study the relevance of H 2 receptors and depression, we treated the CSD group with cimetidine and famotidine respectively. The data showed that cimetidine inhibited depression-like behavior in CSD rats, and famotidine had no impact on depression. Overall our data suggested that the hypothalamic-pituitary-adrenal (HPA) axis dysfunction may be the key role in triggering depression and stress ulcer. Acid-suppressing drugs and antidepressants could be used for treatment of depression and stress ulcer respectively. The occurrence of depression might be inhibited by blocking the central H 2 receptors. Key words: Stress ulcer; Depression; Chronic stress; Acute stress; HPA axis; Histamine H 2 -receptor antagonist 应激诱导的胃溃疡和慢性应激导致的抑郁之间的双向交叉对话 摘要 应激可导致包括抑郁和消化性溃疡在内一系列的疾病和生理紊乱。本研究利用慢性应激抑郁( CSD )及慢性心理应激性溃疡( CPSU )大鼠模型来探讨应激性溃疡和抑郁两种疾病在病理机制和治疗上可能存在的相互关系。结果显示:经慢性应激抑郁处理的大鼠,其溃疡指数明显高于对照组,经慢性心理应激性溃疡处理的大鼠表现出明显的抑郁样行为。氟西汀可以明显降低慢性心理应激性溃疡组大鼠的溃疡指数,而雷尼替丁可以抑制慢性应激抑郁组大鼠的抑郁样行为的发生。分别用米非司酮处理慢性心理应激性溃疡组和慢性应激抑郁组,结果显示慢性心理应激性溃疡组大鼠的溃疡指数明显降低,但两组的抑郁样行为和对照组并无差异。我们同时发现慢性心理应激性溃疡组和慢性应激抑郁组大鼠的血清皮质酮水平低于对照组,而急性应激组大鼠的血清皮质酮水平高于对照组。此外,本实验分别用西咪替丁和法莫替丁处理慢性应激抑郁组大鼠,以探讨抑郁样行为的发生与 H 2 受体之间的关联性。结果显示西咪替丁可以抑制抑郁样行为的发生,但法莫替丁并无此作用。 综上所述,本研究提示 HPA 轴功能障碍可能在抑郁和应激性溃疡的发病机制中起到关键作用。同时提示抑酸药可能发挥抗抑郁作用,而抗抑郁药可以用于治疗应激性溃疡。阻断中枢 H 2 受体可能会抑制抑郁的发生。 关键词: 应激性溃疡; 慢性应激; 急性应激; HPA 轴; 组胺 H2 受体拮抗剂