氢气对体外循环的治疗作用.pdf 其实关于氢气对体外循环后系统炎症治疗作用的研究，国内早就有一些单位开展，但一直没有见到文章发表。这个文章作者分别来自日本和澳大利亚，可以说具有重要的象征意义，因为关于氢气生物学效应的研究,从开始只有美国和亚洲国家，到今年有欧洲和澳洲学者参与，这样除非洲和南美洲以外，其他各大洲都有学者进行了氢气治疗疾病的研究，这说明这一现象得到广泛的认可和关注。这给氢气医学转化到临床奠定了很好的基础。 这一文章发表在《人工器官》 Artif Organs ，澳大利亚作者来自墨尔本的莫纳什大学。在心脏外科手术时经常采用体外循环技术，系统炎症是体外循环经常遇到的问题，也是导致患者死亡的重要原因。根据氢气的抗凋亡和抗炎症作用，作者提出呼吸氢气可能对体外循环引起的系统炎症具有治疗作用。在这一研究中，采用大鼠体外循环模型。动物被随机分成 3 组，假手术组，对照组进行体外循环手术并维持 60 分钟，呼吸氢气组另外增加通过吸氧装置呼吸氢气 60 分钟。分别于手术后 20 分钟和 60 分钟采集血清，并检测肿瘤坏死因子、白细胞介素 6 和白细胞介素 10 等细胞因子，生物化学检测乳酸脱氢酶、天冬氨酸转氨酶和丙氨酸转氨酶，采用干湿重法检测 60 分钟后肺水肿。结果发现，和假手术组比较，体外循环 20 分钟血清细胞因子和生物化学指标均显著升高，体外循环 60 分钟升高更明显。氢气呼吸组体外循环 60 分钟上述指标均显著降低，肺干湿比也有明显改善。结果显示，呼吸氢气可对抗体外循环引起的系统炎症，减少肺水肿。 尽管氢气的抗炎症效果早就被多家研究证实，但对体外循环引起的系统炎症，本研究尚属首次报道。没有看到全文，更详细的内容不太了解。敬请期待。 Artif Organs. 2012 Sep 30. doi: 10.1111/j.1525-1594.2012.01535.x. Insufflation of Hydrogen Gas Restrains the Inflammatory Response of Cardiopulmonary Bypass in a Rat Model. Fujii Y, Shirai M, Inamori S, Shimouchi A, Sonobe T, Tsuchimochi H, Pearson JT, Takewa Y, Tatsumi E, Taenaka Y. Source Departments of Cardiac Physiology Artificial Organs Research and Development Initiative Center, National Cerebral and Cardiovascular Center Research Institute Graduate School of Medicine, Osaka University, Osaka Department of Clinical Engineering, Faculty of Health Sciences, Hiroshima International University, Hiroshima, Japan Department of Physiology, and Monash Biomedical Imaging Facility, Monash University, Melbourne, Australia. Abstract Systemic inflammatory responses in patients receiving cardiac surgery with the use of the cardiopulmonary bypass (CPB) significantly contribute to CPB-associated morbidity and mortality. We hypothesized that insufflated hydrogen gas (H(2) ) would provide systemic anti-inflammatory and anti-apoptotic effects during CPB, therefore reducing proinflammatory cytokine levels. In this study, we examined the protective effect of H(2) on a rat CPB model. Rats were divided into three groups: the sham operation (SHAM) group, received sternotomy only; the CPB group, which was initiated and maintained for 60 min; and the CPB + H(2) group in which H(2) was given via an oxygenator during CPB for 60 min. We collected blood samples before, 20 min, and 60 min after the initiation of CPB. We measured the serum cytokine levels of (tumor necrosis factor-α, interleukin-6, and interleukin-10) and biochemical markers (lactate dehydrogenase, aspartate aminotransferase, and alanine aminotransferase). We also measured the wet-to-dry weight (W/D) ratio of the left lung 60 min after the initiation of CPB. In the CPB group, the cytokine and biochemical marker levels significantly increased 20 min after the CPB initiation and further increased 60 min after the CPB initiation as compared with the SHAM group. In the CPB + H(2) group, however, such increases were significantly suppressed at 60 min after the CPB initiation. Although the W/D ratio in the CPB group significantly increased as compared with that in the SHAM group, such an increase was also suppressed significantly in the CPB + H(2) group. We suggest that H(2) insufflation is a possible new potential therapy for counteracting CPB-induced systemic inflammation. 2012, Copyright the Authors. Artificial Organs 2012, International Center for Artificial Organs and Transplantation and Wiley Periodicals, Inc. PMID: 23020073
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