业绩 (微型小说) 某省的一些下辖市正在召开着一年一度的两会。 A 市的《政府工作报告》在总结上一年度的工作成就时谈及,将一大型污染化工企业成功从本市辖区迁出,虽损失了一定的GDP,但却保住了青山绿水,成就巨大,可喜可贺! B 市的《政府工作报告》在总结上一年度的工作成就时谈及,成功引进了一家大型化工企业,对本市的GDP贡献极大,且新增不少就业机会,可喜可贺! 据知情人士透露,B市所引进的化工企业正是从A市迁出的那一家污染企业。
这样的底气从何而来? 蒋继平 上个星期, 是我的实验站建立以来的第一次综合评估。这个评估是由总公司的核心领导和总公司聘请的第三方一起执行的。 这个考核评估在两个月之前就通知了我们。 当时就有一个临时的行程表。 这个行程表包括考核的内容, 会议的时间, 和参加人员等具体的细节。 大约一个月前, 这个行程表又做了一些修改。 到了上个星期, 这个行程表又做了一些变动。 按照原先的安排, 10 月 17 日, 星期三上午由我们实验站提供综合报告。 这个报告是由实验站主任, 也是我的老板负责。 我们其他雇员给与适当的补充, 下午是参观实验室, 人工气候箱, 温室和田间试验点。 10 月 18 日, 星期四是对每个人的单独交流和考核。不知道什么原因, 他们临时改变了原来的计划。 改变后的行程是星期三上午参观实验室, 人工气候箱, 温室和田间试验点, 下午是一些人员介绍和综合性的讨论,星期四是综合报告, 星期五是个人评审和交流。 公司的核心领导一共来了三位最高主管, 他们分别是行政第一执行官( CEO ) , 日常运作第一执行官( COO ) , 和公司首席经济师( CFO )。 外聘的是两位专家。 一位是 Dr. Ed Green 。 Green 博士曾经是孟三都( Monsanto ) 公司负责蔬菜研究的主管, 现在已经退休, 退休后他自己办了一个资询公司。 另一位是 Mark Nelson. Nelson 先生是 Context 公司的合伙人, 是专门从事各种考核评估的专业人员。我和公司的三位主管算是老熟人, 和 Ed Green 博士也是老熟人。 这是因为我和他当年都是圣尼斯( Seminis ) 的雇员, 我们在同一个实验站共事过几年时间, 当时他是圣尼斯公司生物工程( Biotechnology )部门的负责人。 因而,在这些考核人员中, 只有 Nelson 先生是第一次见面。 星期三我只是简单地和他们握手, 互通姓名, 让他们参观我的实验室, 人工气候箱, 温室等相关的设施。 星期四主要是参加实验站的综合报告, 在一些细节和关键的技术问题上给与老板一些补充说明。 星期五早上我像往常一样 8 点前几分钟到达实验站。 当我走进实验大楼后, 发现整个会议室已经坐满了人。 这些人包括 外聘的两位专家, 和我们实验站的核心雇员。 这天的主要议程是个人的考核。 我走进自己的办公室, 在办公桌上写了一个简单的纸条:“ Ed and Mark: I am very sorry to say that I have to go to the lab to do two important tests. These tests are very critical to my job. And these tests are time sensitive. If you have specific questions for me, please come to the lab to talk to me later when you finished your interviews with other employees 。 ( Ed and Mark, 很抱歉,我得在实验室作两个重要的试验,这些试验对我的工作很重要, 这些试验对时间很敏感。假如你们有特殊的问题要问我, 请在你们完成了对其他雇员的评审后来实验室找我。 )” 我走进会议室, 简单地与大家打个招呼后, 就将写好的纸条放在 Ed 和 Mark 的面前, 他们两人看了后笑嘻嘻地朝我挥挥手, 让我赶紧回实验室。 到了 11 点左右, 我仍然在实验室忙着我的实验, 他们的个人考核已经结束。 他们没有来实验室找我谈话, 而是直接回公司总部汇报他们的工作去了。 到了下午两点左右, 我接到老板的电邮, 他说他刚接到公司总部财务处的通知, 要我查查我的银行账号, 是不是已经收到公司给我的红利( Bonus ), 不管是否收到, 他要我立即给公司财务处一个答复。 我接到老板的电邮, 有点不相信老板的信息。 这是因为我今年一月份已经收到过公司的红利。 公司的规定是每年一次红利分配。 这个时候不是红利分配的时候, 而且也不可能给与第二次红利。 但是, 我知道我的老板一般不会跟我开这样的玩笑的, 因而,我立即打电话给太太, 请她立即查查我的银行账号, 是不是真的。 顺便说一下, 我已经五年多从来不问家中经济上的事, 以至于银行中有多少钱, 我的账号密码等等我都不知道。 太太接到我的电话后, 立即查了我的银行账号, 并在第一时间用电邮通知我是真的, 她说大约一个小时前,在我的银行账号上出现了一个与平时工资不一样的进账。 那天下午下班回到家, 太太问我为什么公司在这个时候给我发红利。我说我也不知道到底是什么回事, 接着我跟她谈论了早上发生的事。 太太问我为什么会不顾自己的个人考核? 这样的底气从何而来? 面对太太的追问, 我觉得一言难尽。 为了简单起见, 我只能告许太太, 我知道他们已经看到了我的实验室, 看到了我的人工气候箱内的试验,看到了我的整个温室内的实验, 他们是这方面的专家, 他们看到了事实, 因而,用不着我做更多的解释,所以, 用不着浪费我宝贵的时间。 而且, 是他们一再改变原来的时间安排。 我的实验安排是有严格的时间局限性的, 一般不可以任意改动, 否则的话, 可能影响到实验的结果。进一步来说, 在两个大型人工气候箱内, 在一个温室内, 种植着几百盆植物, 总计有几万株植物, 它们处于不同的生长期, 它们被好多种不同的菌种接种, 表现出不同的反应, 这些反应几乎是像设计好了似的, 近于理想的完美。 这不是论文可以表达的, 也不是口头汇报可以表达的, 更不是吹牛可以吹出来的, 这些都是活生生的植物。 借此机会, 我顺便给太太上了一堂课。 我对她说,真正的专家和内行是心灵相通的, 他们看到了事实, 他们知道这些事实背后的真相, 他们也知道要获得如此的结果, 必须达到什么样的水平和付出什么样的努力。 不是我喜欢吹牛, 任何专家内行看到我的人工气候箱内和温室内的实验结果, 他们一定会心服口服的, 越是专家, 越是如此!这就是我的底气, 这种底气是平时知识, 经验和汗水的结晶, 具体地反映在温室和人工气候箱内的试验品上。
最近,看到两个现象: 1.有些大学生不努力 2.心比天高,命比纸薄 发现一个怪圈---美女爱英雄! 美女谁都爱,可是美女愿意屈尊于庸俗之辈吗? 转载一篇博文: 自然选择给地球人带来的好处 牛顿,爱因斯坦,达尔文对世界的影响最大。其中,达尔文的进化论对世界影响更大。达尔文认为,自然选择是适者生存。 最近,一位上海交通大学的学生向我提出了一个很好的问题:美国教授最希望的是什么样的学生?(我的答案:业绩为上,业绩优先。) 我介绍我很熟悉的两个美国教授。一个是 Min Ni ,一个是 Jiaxu Li 。他们做 Assistant professor 很艰苦。我理解他们。一次,我们见面时,都是会心地一笑。因为,我们在一起,也算同行。他们的学生,我的学生,我们知道学生们会交流心得。学生们都是从老师们的成功之路上寻找足迹,受到启发。我也在从学生们的眼神中寻找答案。 1. 学生们愿意走我们的路吗? 2. 学生们愿意付出我们的辛苦吗? 3. 我们的追求是学生们的追求吗? 4. 我们的成功之路学生们可以复制吗? 5. 有的学生选择自主创业,有的愿意倾听老师意见,学生们的路线图是什么? 6. 选择老师,实际上还直接或间接选择未来。在这方面学生们如何考虑? 归结为一点:业绩为上! 这里介绍两位教授,他们都有 CNS 。而且,都是通吃(顶尖杂志论文大丰收)! 附件一:李家旭业绩 Dr. Jiaxu Li Assistant Professor of Biochemistry and Molecular Biology Education: Shenyang Normal University , B.S. in Biology, 1986. Hebei Normal University , M.S. in Biochemistry, 1989. Pennsylvania State University , Ph.D. in Plant Physiology, 1998. Pennsylvania State University , Post-doctoral Scholar, 08/1998-04/2001. Harvard Medical School , Post-doctoral Fellow, 05/2001-08/2003. Areas of Research: A fundamental problem in biology is how cells sense and respond to stimuli. Precise sensing and proper responding to environmental changes are of particular importance for plants' survival, because plants are constantly exposed to highly variable conditions (i.e. temperature, light, water status and nutrient) and they cannot move to escape unfavorable or stressful environment. A better understanding of the mechanism of how plant cells sense and respond to environmental stimuli could lead to new strategies for improving stress tolerance of crop plants. We wish to understand aspects of signal processing and transduction pathways in dehydration stress induced by high salt, drought. The plant hormone, abscisic acid (ABA), is involved in regulation of some stress responses. We are particularly interested in molecular elucidation of regulatory protein components critical in ABA-controlled stress responses using proteomics and genomics approaches. Selected Publications Ho, H.Y., Rohatgi, R., Lebensohn, A.M., Ma, L., Li J, Gygi, S.P., and Kirschner, M.W. (2004). Toca-1 Mediates Cdc42-Dependent Actin Nucleation by Activating the N-WASP-WIP Complex. Cell 118:203-216. Gautreau, A., Ho, H., Li , J., Steen, H., Gygi, S.P., and Kirschner, M.W. (2004). Purification and architecture of the ubiquitous Wave Complex. Proc Natl Acad Sci USA 101:4379-4383. Beausoleil, S.A., Jedrychowski, M., Schwartz, D., Elias, J.E., Villen, J., Li , J., Cohn, M.A., Cantley, L.C., and Gygi, S.P. (2004). Large-scale characterization of HeLa cell nuclear phosphoproteins. Proc Natl Acad Sci U S A 101:12130-12135. Li , J., Steen H., and Gygi, S.P. (2003). Protein profiling with cleavable isotope coded affinity tag (cICAT) reagents: the yeast salinity stress response. Molecular and Cellular Proteomics 2:1198-1204. Li , J., Kinoshita, T., Pandey, S., Ng, C. K., Gygi, S.P., Shimazaki, K., and Assmann, S. M. (2002). Modulation of an RNA-binding protein by abscisic-acid-activated protein kinase. Nature 418: 793-797. Li , J., Moazed, D., and Gygi, S.P. (2002). Association of the histone methytransferase Set2 with RNA polymerase II regulates transcription elongation. Journal of Biological Chemistry 277: 49383-49388. Li , J., Wang, X.-Q., Watson, M. B., and Assmann, S. M. (2000). Regulation of abscisic acid-induced stomatal closure and anion channels by guard cell AAPK kinase. Science 287: 300-303. Li , J. and Assmann, S. M. (2000). Mass spectrometry: An essential tool in proteome analysis. Plant Physiology 123: 807-809. Li , J., Lee, Y.-R., and Assmann, S. M. (1998). Guard cells possess a calcium-dependent protein kinase that phosphorylates the KAT1 K+ channel. Plant Physiology 116: 785-795. Li , J., and Assmann, S. M. (1996). An abscisic acid-activated and calcium-independent protein kinase from guard cells of fava bean. Plant Cell 8: 2359-2368. Communication Links Phone: (662) 325-1115 Fax: (662) 325-8664 Internet: JL305@Ra.MsState.edu 附件二: Min Ni 业绩 Ph.D., University of Oklahoma, 1992 Light signal transduction and photomorphogenesis Contact Information Mailing Address: Dr. Min Ni Department of Plant Biology University of Minnesota 250 Biological Sciences Center 1445 Gortner Avenue St. Paul, MN 55108 Office: 644 Biological Sciences Center Phone: 612-625-3702 Fax: 612-625-1738 E-mail: nixxx008@umn.edu Web Site: Min Ni Lab Research Interests Plants sense their ambient light conditions through a series of photoreceptors. The red/far-red light-absorbing phytochromes and UV-A/blue light-absorbing crytochromes regulate seedling de-etiolation, photoperiodic flowering, and circadian rhythm. The de-etiolation responses include the inhibition of hypocotyl elongation, the opening of cotyledons and hypocotyl hooks, and the development of chloroplasts. To understand the signaling events downstream of the photoperception, we have identified several mutants that have defective seedling de-etiolation responses under multiple wavelengths. The first mutant family includes recessive hrb ( hypersensitive to red and blue ) mutants that were initially isolated for their short hypocotyl phenotype under red and blue light. We have cloned HRB1 and mapped hrb2 and hrb3 to chromosome 2 and chromosome 4, respectively. HRB1 encodes a nuclear protein that contains a ZZ-type zinc finger domain implicated in protein-protein interactions in other organisms. HRB1 activity is also required for red and blue light-induced expression of PIF4 . Like hrb1, pif4 is hypersensitive to both red and blue light and pif4 is epistatic to hrb1. Thus, HRB1 and PIF4 may define points where red light signaling and blue light signaling intersect. In addition to its de-etiolation phenotypes, hrb1 also flowers late and attenuates the expression of FT , an integrator of several flowering induction pathways, under both long days and short days. In contrast, transgenic plants that overexpress full-length HRB1 or its C-terminal half flower early and accumulate more FT mRNA under short days. hrb1 is epistatic to cry2 in long-day flowering and FT expression, whereas phyB is epistatic to hrb1 in both long-day and short-day flowering and FT expression. HRB1 thus may act downstream of cry2 but modulates phyB signaling for photoperiodic flowering. Another knockout mutant is short hypocotyl under blue 1 or shb1 . However, shb1-D , a dominant gain-of-function allele due to the over-accumulation of SHB1 transcript, exhibited a long hypocotyl phenotype under red, far-red, and blue light. Therefore, SHB1 acts in blue light signaling and overexpression of SHB1 may expand its signaling activity to red and far-red light. Consistent with this, overexpression of SHB1 enhances expression of PIF4 under red light. PIF4 appears to specifically mediate SHB1 regulation of hypocotyl elongation and CAB3 or CHS expression under red light. Overexpression of SHB1 also promotes proteasome-mediated degradation of phyA and hypocotyl elongation under far-red light. Under blue light, shb1 suppresses HFR1 expression and shows several de-etiolation phenotypes similar to hfr1-201 . However, the hypocotyl and cotyledon-opening phenotypes of shb1 are opposite to those of hfr1-201 , and HFR1 acts downstream of SHB1. SHB1 encodes a nuclear and cytosolic protein that contains SPX and EXS domains homologous to the SYG1 protein family. The function of the two domains and their interactions with other proteins remain largely unknown. We demonstrated that overexpression of the N-terminal 520 amino acids, harboring the intact SPX domain, caused a hyposensitive hypocotyl phenotype similar to that of shb1-D . In contrast, overexpression of several C-terminal truncations, all including the intact EXS domain, created a dominant-negative hypersensitive hypocotyl phenotype similar to that of shb1 . To study how SHB1 interacts with other genes, we have isolated a series of suppressors and enhancers of shb1-D that carry mutations either in the SHB1 gene or in different genetic loci. Since the genetic suppressors and enhancers may define proteins that directly interact with SHB1, we have conducted a preliminary screen of a yeast two-hybrid library for SHB1 interacting proteins. Selected Publications Kang, X., Zhou, Y., Sun, X., and Ni, M. (2007). HYPERSENSITIVE TO RED AND BLUE 1 and its C-terminal regulatory function define a signaling step that controls FLOWERING LOCUS T expression. Plant Journal (in press). Zhou, Y., Sun, X., and Ni, M. (2007). Timing of photoperiodic flowering: light perception and circadian clock. J Integr Plant Biol 49, 28-34. Chen, M., and Ni, M. (2006). RED AND FAR-RED INSENSITIVE 2, a RING-domain zinc-finger protein, negatively regulates CONSTANS expression and photoperiodic flowering. Plant Journal 46, 823-833. Kang, X., and Ni, M. (2006). Arabidopsis SHORT HYPOCOTYL UNDER BLUE 1 contains SPX and EXS domains and acts in cryptochrome signaling. Plant Cell 18, 921-934. Chen, M., and Ni, M. (2006). RED AND FAR-RED INSENSITIVE 2, a RING-domain zinc-finger protein, mediates phytochrome-controlled seedling de-etiolation responses. Plant Physiology 140, 457-465. Ni, M. (2005). Integration of light signaling with photoperiodic flowering and circadian regulation. Cell Research 15, 559-566. Ni, M . (2005). Downstream integrators of red, far-red, and blue light signaling for photomorphogenesis. In Light Sensing in Plants , M. Wada, K. Shimazaki, and M. Iino eds. ( Sringer-Verlag, Tokyo), 293-298. Kang, X., Chong, J., and Ni, M . (2005). HYPERSENSITIVE TO RED AND BLUE 1, a ZZ-type zinc finger protein, regulates phytochrome B-mediated red and cryptochrome-mediated blue light responses. Plant Cell 17, 822-835. Ni, M. , Tepperman, J., and Quail, P.H. (1999). Binding of phytochrome B to its nuclear signaling partner PIF3 is reversibly induced by light. Nature 400, 781-784. Halliday, K., Hudson, M., Ni, M. , Qin, M., and Quail, P.H. (1999). poc1: an Arabidopsis mutant perturbed in phytochrome signaling due to a T-DNA insertion in the promoter of PIF3, a gene encoding a phytochrome-interacting, bHLH protein. PNAS 96, 5832-5837. Ni, M., Tepperman, J., and Quail, P.H. (1998). PIF3, a phytochrome interacting factor necessary for photoinduced signal transduction, is a basic helix-loop-helix protein. Cell 95, 657-667. Ni, M. , Dehesh, K., Tepperman, J., and Quail, P.H. (1996). GT-2: In vivo transcriptional activation activity and definition of twin novel DNA-binding domains with reciprocal target-site selectivity. Plant Cell 8, 1041-1059.