iNature ：对于全球最高引的前100篇文章，篇均引用量是 34834.92 次；这100篇文章主要聚集在 生物化学及分子生物学领域；这100篇文章中， 发表在PHYSICAL REVIEW B杂志及JBC杂志都是发表了7篇文章；对于这100篇文章，产出率最高的机构是加州大学；总的来说，这100篇文章主要是聚集在基础研究， 如蛋白质的定量，蛋白质的分析，实时定量PCR技术，BLAST的诞生，DNA测序，同源序列比对等方面。 iNature编辑组的统计时间是2017年11月26日。 1 引用量最高的10000篇文章分析 我们以Web of Science为基础，通过相关的检索，从1874-2018年，总共检索到 120611782条记录（ 图.1 ）。 图.1 总文章数 我们对于所有的文章进行了分类归档，发现引用次数大于10万次，为第一档，有3篇文章；第二档的引用量是5-10万次，有9篇文章；第三档的引用量是1-5万次，有238篇文章；第四档的引用量是5000-1万次，有748篇文章；第五档的引用量是1000-5000次，有22994篇文章；第六档的引用量是500-1000次，有68509篇文章； 第七档的引用量是小于500次，有120519371篇文章 （ 图.2 ） 。 文章分档 引用次数 文章数 累加文章数 I 10万 3 II 5-10万 9 12 III 1-5万 238 250 IV 5000-1万 748 998 V 1000-5000 22994 23902 VI 500-1000 68509 92411 VII 500 120519371 120611782 图.2 所有文章的归档分类 之后我们根据引用量从高到底，进行排序，我们筛选了10000条记录（因为Web of Science最多只能一次分析10000条记录），进行相应的统计分析。 我们发现每篇文章的平均引用次数是3173.14次，总引用次数是31731393次，施引文献是14692585 （ 图.3 ） 。另外，我们发现，最高引用文章大部分都聚集在1990-2010年之间，达到了40%以上。 图.3 10000篇文章引用分析 之后，我们看了一下不同时间段，对于这些文章的引用次数分析，发现绝大部分引用都是聚集在1990-2016年之间（ 图.4 ）。 图.4 10000篇文章不同时间段引用分析 由于分析10000篇文章，工作量太大，我们就直接分析前100篇文章，发现前100篇文章的平均引用量是34834.92次，总引用次数是3483492次，施引文献达到2627346篇。这100篇文章，占前10000篇的总引用的10.98%，但是总文章数是1.00%，故我们主要分析这100篇文章 （ 图.5 ） 。 图.5 100篇文章引用分析 2 引用量最高的100篇文章分析 对于这100文章发表的时间进行分析，我们发现在1950年以前只有4篇，1950-1960年之间，有10篇；1961-1970年，有9篇；1971-2010年，总共有74篇，占了绝大部分；2010年以后，只有3篇文章（ 图.6 ），这三篇文章分别是遗传进化（ MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods，2011年），癌症综述（ Hallmarks of Cancer: The Next Generation，2011年 ），全球癌症统计（ Global Cancer Statistics，2011年 ） 。 图.6 100篇文章发表时间分析 其次我们对这100篇文章的种类进行了基本分析，发现生物化学及分子生物学方面有35篇，物理学有15篇，化学有14篇，数学11篇 （ 图.7 ） 。 图.7 100篇文章方向分析 我们再次对这100篇文章发表的杂志进行分析（3篇），发表在PHYSICAL REVIEW B杂志及JBC杂志都是7篇文章；ANALYTICAL BIOCHEMISTRY，JOURNAL OF CHEMICAL PHYSICS，NATURE，NUCLEIC ACIDS RESEARCH都是发表了4篇文章，总的来说，这高引用的100篇文章，大部分都是生物，物理，生物化学及化学方面的文章。另外这9个杂志占了39篇文章 （ 图.8 ） 。 图.8 100篇文章杂志分析 我们再次统计了国家，发现美国有44篇，占了绝大部分，其次是英国，达到9篇；德国有6篇，很遗憾，没有发现有中国参与的文章 （ 图.9 ） 。 图.9 100篇文章国家分布 我们对研究机构统计了一下，发现加州大学系统占的比例最大，达到了7篇，其次是宾夕法尼亚州立大学，达到了4篇 （ 图.10 ） 。 图.10 100篇文章的大学或研究所分析 3 引用量最高的100篇文章列表 对于这100篇文章，主要是技术的变革及基础研究，如蛋白质的定量，蛋白质的分析，实时定量PCR技术，BLAST的诞生，DNA测序，同源序列比对等，这些东西都是非常的基础，故这也注定了它们的高引用量 （ 图.11 ） 。 标题 总引用次数 年均引用 1 PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT 335844 5012.6 2 CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 244785 5099.69 3 RAPID AND SENSITIVE METHOD FOR QUANTITATION OF MICROGRAM QUANTITIES OF PROTEIN UTILIZING PRINCIPLE OF PROTEIN-DYE BINDING 201313 4793.17 4 DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS 66790 1629.02 5 DENSITY-FUNCTIONAL THERMOCHEMISTRY .3. THE ROLE OF EXACT EXCHANGE 65244 2609.76 6 Generalized gradient approximation made simple 63484 2885.64 7 SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION 63161 2037.45 8 DEVELOPMENT OF THE COLLE-SALVETTI CORRELATION-ENERGY FORMULA INTO A FUNCTIONAL OF THE ELECTRON-DENSITY 61406 2046.87 9 A short history of SHELX 58640 5864 10 Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(-Delta Delta C) method 58159 3421.12 11 ELECTROPHORETIC TRANSFER OF PROTEINS FROM POLYACRYLAMIDE GELS TO NITROCELLULOSE SHEETS - PROCEDURE AND SOME APPLICATIONS 55121 1413.36 12 A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES 51444 843.34 13 BASIC LOCAL ALIGNMENT SEARCH TOOL 49116 1754.14 14 CLUSTAL-W - IMPROVING THE SENSITIVITY OF PROGRESSIVE MULTIPLE SEQUENCE ALIGNMENT THROUGH SEQUENCE WEIGHTING, POSITION-SPECIFIC GAP PENALTIES AND WEIGHT MATRIX CHOICE 47444 1976.83 15 Gapped BLAST and PSI-BLAST: a new generation of protein database search programs 45557 2169.38 16 NONPARAMETRIC-ESTIMATION FROM INCOMPLETE OBSERVATIONS 44604 743.4 17 MINI-MENTAL STATE - PRACTICAL METHOD FOR GRADING COGNITIVE STATE OF PATIENTS FOR CLINICIAN 43637 1014.81 18 A REVISED MEDIUM FOR RAPID GROWTH AND BIO ASSAYS WITH TOBACCO TISSUE CULTURES 41340 738.21 19 THE NEIGHBOR-JOINING METHOD - A NEW METHOD FOR RECONSTRUCTING PHYLOGENETIC TREES 38735 1249.52 20 A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION 37927 642.83 21 REVISED EFFECTIVE IONIC-RADII AND SYSTEMATIC STUDIES OF INTERATOMIC DISTANCES IN HALIDES AND CHALCOGENIDES 37143 884.36 22 DENSITY-FUNCTIONAL EXCHANGE-ENERGY APPROXIMATION WITH CORRECT ASYMPTOTIC-BEHAVIOR 34065 1135.5 23 SELF-CONSISTENT EQUATIONS INCLUDING EXCHANGE AND CORRELATION EFFECTS 33872 639.09 24 Efficient iterative schemes for ab initio total-energy calculations using a plane-wave basis set 33658 1529.91 25 The moderator-mediator variable distinction in social psychological research: conceptual, strategic, and statistical considerations. 33191 1037.22 26 Processing of X-ray diffraction data collected in oscillation mode 32667 1555.57 27 The status, quality, and expansion of the NIH full-length cDNA project: The Mammalian Gene Collection (MGC) 32391 2313.64 28 DETECTION OF SPECIFIC SEQUENCES AMONG DNA FRAGMENTS SEPARATED BY GEL-ELECTROPHORESIS 32239 749.74 29 REGRESSION MODELS AND LIFE-TABLES 32022 696.13 30 COLORIMETRIC METHOD FOR DETERMINATION OF SUGARS AND RELATED SUBSTANCES 31882 514.23 31 RAPID COLORIMETRIC ASSAY FOR CELLULAR GROWTH AND SURVIVAL - APPLICATION TO PROLIFERATION AND CYTO-TOXICITY ASSAYS 31292 894.06 32 The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools 30770 1465.24 33 HELICAL MICROTUBULES OF GRAPHITIC CARBON 30386 1125.41 34 STATISTICAL METHODS FOR ASSESSING AGREEMENT BETWEEN TWO METHODS OF CLINICAL MEASUREMENT 29847 932.72 35 FUZZY SETS 29740 561.13 36 CONTROLLING THE FALSE DISCOVERY RATE - A PRACTICAL AND POWERFUL APPROACH TO MULTIPLE TESTING 29691 1290.91 37 Electric field effect in atomically thin carbon films 28520 2037.14 38 INHOMOGENEOUS ELECTRON GAS 27972 518 39 CONFIDENCE-LIMITS ON PHYLOGENIES - AN APPROACH USING THE BOOTSTRAP 27721 840.03 40 SPECIAL POINTS FOR BRILLOUIN-ZONE INTEGRATIONS 27045 643.93 41 MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods 26581 3797.29 42 USE OF LEAD CITRATE AT HIGH PH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPY 25405 461.91 43 From ultrasoft pseudopotentials to the projector augmented-wave method 25057 1318.79 44 MEASUREMENT OF OBSERVER AGREEMENT FOR CATEGORICAL DATA 25005 609.88 45 PROJECTOR AUGMENTED-WAVE METHOD 24314 1013.08 46 MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0 24066 2187.82 47 RELIABILITY OF MOLECULAR WEIGHT DETERMINATIONS BY DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS 23835 486.43 48 THE ATTRACTIONS OF PROTEINS FOR SMALL MOLECULES AND IONS 23763 344.39 49 ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE 23574 604.46 50 The colorimetric determination of phosphorus 23420 251.83 51 Cutoff Criteria for Fit Indexes in Covariance Structure Analysis: Conventional Criteria Versus New Alternatives 22755 1197.63 52 Particle swarm optimization 22586 982 53 DISC ELECTROPHORESIS .2. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS 22463 415.98 54 MAXIMUM LIKELIHOOD FROM INCOMPLETE DATA VIA EM ALGORITHM 22259 542.9 55 A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY 21557 615.91 56 Efficiency of ab-initio total energy calculations for metals and semiconductors using a plane-wave basis set 21539 979.05 57 NEW LOOK AT STATISTICAL-MODEL IDENTIFICATION 21172 481.18 58 Global Cancer Statistics 20983 2997.57 59 A NEW GENERATION OF CA-2+ INDICATORS WITH GREATLY IMPROVED FLUORESCENCE PROPERTIES 20677 626.58 60 CLINICAL-DIAGNOSIS OF ALZHEIMERS-DISEASE - REPORT OF THE NINCDS-ADRDA WORK GROUP UNDER THE AUSPICES OF DEPARTMENT-OF-HEALTH-AND-HUMAN-SERVICES TASK-FORCE ON ALZHEIMERS-DISEASE 20596 605.76 61 THE ASSESSMENT AND ANALYSIS OF HANDEDNESS: THE EDINBURGH INVENTORY 20514 436.47 62 The rise of graphene 20505 1864.09 63 Distinctive image features from scale-invariant keypoints 20258 1447 64 A RATING SCALE FOR DEPRESSION 20238 348.93 65 AN INVENTORY FOR MEASURING DEPRESSION 20104 352.7 66 EQUATION OF STATE CALCULATIONS BY FAST COMPUTING MACHINES 19396 298.4 67 ESTIMATION OF CONCENTRATION OF LOW-DENSITY LIPOPROTEIN CHOLESTEROL IN PLASMA, WITHOUT USE OF PREPARATIVE ULTRACENTRIFUGE 19380 421.3 68 HIGH-RESOLUTION 2-DIMENSIONAL ELECTROPHORESIS OF PROTEINS 19179 446.02 69 COMPARISON OF SIMPLE POTENTIAL FUNCTIONS FOR SIMULATING LIQUID WATER 19023 543.51 70 THE MOS 36-ITEM SHORT-FORM HEALTH SURVEY (SF-36) .1. CONCEPTUAL-FRAMEWORK AND ITEM SELECTION 18987 730.27 71 MicroRNAs: Genomics, biogenesis, mechanism, and function 18845 1346.07 72 PHASE ANNEALING IN SHELX-90 - DIRECT METHODS FOR LARGER STRUCTURES 18787 670.96 73 A LOW-COST, HIGH-EFFICIENCY SOLAR-CELL BASED ON DYE-SENSITIZED COLLOIDAL TIO2 FILMS 18733 693.81 74 GAUSSIAN-BASIS SETS FOR USE IN CORRELATED MOLECULAR CALCULATIONS .1. THE ATOMS BORON THROUGH NEON AND HYDROGEN 18531 639 75 OPTIMIZATION BY SIMULATED ANNEALING 18374 524.97 76 A NEW METHOD OF CLASSIFYING PROGNOSTIC CO-MORBIDITY IN LONGITUDINAL-STUDIES - DEVELOPMENT AND VALIDATION 18044 582.06 77 MrBayes 3: Bayesian phylogenetic inference under mixed models 17994 1199.6 78 TISSUE SULFHYDRYL GROUPS 17989 304.9 79 VMD: Visual molecular dynamics 17956 816.18 80 METAANALYSIS IN CLINICAL-TRIALS 17945 560.78 81 IMPROVED PATCH-CLAMP TECHNIQUES FOR HIGH-RESOLUTION CURRENT RECORDING FROM CELLS AND CELL-FREE MEMBRANE PATCHES 17744 479.57 82 HOMEOSTASIS MODEL ASSESSMENT - INSULIN RESISTANCE AND BETA-CELL FUNCTION FROM FASTING PLASMA-GLUCOSE AND INSULIN CONCENTRATIONS IN MAN 17634 534.36 83 A SIMPLE METHOD FOR DISPLAYING THE HYDROPATHIC CHARACTER OF A PROTEIN 17621 489.47 84 Measuring inconsistency in meta-analyses 17584 1172.27 85 MEASUREMENT OF PROTEIN USING BICINCHONINIC ACID 17573 532.52 86 THE HOSPITAL ANXIETY AND DEPRESSION SCALE 17458 498.8 87 STUDY OF THE CONDITIONS AND MECHANISM OF THE DIPHENYLAMINE REACTION FOR THE COLORIMETRIC ESTIMATION OF DEOXYRIBONUCLEIC ACID 17232 277.94 88 The Protein Data Bank 17152 952.89 89 The NCEP/NCAR 40-year reanalysis project 17118 778.09 90 Collective dynamics of 'small-world' networks 17044 852.2 91 Hallmarks of Cancer: The Next Generation 16913 2416.14 92 ABINITIO MOLECULAR-DYNAMICS FOR LIQUID-METALS 16788 671.52 93 Bias in meta-analysis detected by a simple, graphical test 16785 799.29 94 DETERMINATION OF SERUM PROTEINS BY MEANS OF THE BIURET REACTION 16754 242.81 95 A MATHEMATICAL THEORY OF COMMUNICATION 16715 238.79 96 PROCHECK - A PROGRAM TO CHECK THE STEREOCHEMICAL QUALITY OF PROTEIN STRUCTURES 16710 668.4 97 MODELTEST: testing the model of DNA substitution 16619 830.95 98 MULTIPLE RANGE AND MULTIPLE F TESTS 16580 263.17 99 ESTIMATING DIMENSION OF A MODEL 16551 413.78 100 A SIMPLE METHOD FOR ESTIMATING EVOLUTIONARY RATES OF BASE SUBSTITUTIONS THROUGH COMPARATIVE STUDIES OF NUCLEOTIDE-SEQUENCES 16492 434 图.11 前100篇文章列表 注： 所有的数据来源为Web of science。 温馨提示 ： iNature微信公众号是介绍一流的，最前沿的科研成果，提供专业的完整的同行解析；另外也会介绍全世界知名的实验室及业界大师；同时为公众提供一个了解生命科学及科研过程的平台。
标签: 最高引用
