糖化血清蛋白是诊断糖尿病的重要指标之一。目前糖尿病领域认为，糖化血清蛋白主要体现了血中葡萄糖两周左右的平均水平。但是，我们的实验证明是核糖代谢失调主要决定了糖化血清蛋白的水平。 实验结果 1000 mM 葡萄糖和 20 mM 核糖分别与人血清白蛋白孵育反应两周，经过质谱分析得到如图 1 的结果。 图1、在核糖浓度小于葡萄糖浓度50倍的情况下，核糖糖基化人血清白蛋白的Lys氨基酸残基数（17个）是葡萄糖（7个）2倍多。 Fig. 1. Glycation sites on HSA in the presence of D -ribose and D -glucose analyzed by LC-MS/MS. The conditions of HSA glycation are as shown in Fig. 1 , except that 2-week glycated HSA was employed for mass spectrometry. The glycated samples were processed in 12% SDS-PAGE and sequentially decolorized, reduced and alkylated. The protein bands were digested with trypsin overnight for peptide fragments. Those peptides were then analyzed by LC-MS/MS. Pyrraline (blue); CEL, carboxyethyllysine (yellow); CML, carboxymethyllysine (red); background (CML in white); K, lysine residue. 结果表明，糖化血清蛋白主要被核糖糖化，换句话说，糖化血清蛋白在体内的水平主要体现的是糖尿病病人核糖代谢失调的指标。 参考文献 1. Yao Chen, Lexiang Yu, Yujing Wang, Yan Wei, Yong Xu, Tao He, Rongqiao He. D-Ribose contributes to the glycation of serum protein. BBADis 2019, May 11. doi: 10.1016/j.bbadis.2019.05.005. https://www.ncbi.nlm.nih.gov/pubmed/31085227 2. D-ribose and glycated hemoglobin--- 核糖与糖化血红蛋白 http://blog.sciencenet.cn/blog-829868-1079515.html
标签: 糖化血清蛋白
