2010年度美国国家科学奖章获得者名单公布 http://news.sciencenet.cn//htmlnews/2010/10/238863.shtm , AS Kang, RA Lerner, SJ Benkovic - Proceedings of the , 1991 - National Acad Sciences ... CARLOS F. BARBAS III*, ANGRAY S . KANG*, RICHARD A. LERNER*, AND STEPHEN J. BENKOVICt ... Torrey Pines Road, La Jolla, CA 92037; and tDepartment of Chemistry, Pennsylvania State University, University Park, PA 16802 Contributed by Stephen J. Benkovic , June 21 ... Cited by 747 - Related articles - All 10 versions Accelerated Alzheimer-type phenotype in transgenic mice carrying both mutant amyloid precursor protein and presenilin 1 transgenes , E McGowan, X Yu, S Benkovic , P Jantzen, K Wright - Nature medicine, 1998 - nature.com Genetic causes of Alzheimer's disease (AD) include mutations in the amyloid precursor protein (APP), presenilin 1 (PS1), and presenilin 2 (P52) genes 1 . The mutant APP K670N,M67M transgenic line, Tg2576, shows markedly elevated amyloid -protein (AP) levels at an early age and, ... Cited by 692 - Related articles - BL Direct - All 4 versions Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda , AS Kang, M Alting-Mees, DR Burton, SJ Benkovic , RA - Science, 1989 - sciencemag.org 66. TA Stewart, PK Pattengale, P. Leder, Cell 38, 627 (1984). 67. C. Schoenenberger et al., EMBO J. 7, 169 (1988). 68. U. Ruther, C. Garber, D. Komitowski, R. Muller, EF Wagner, Nature 325, 412 (1987); U. Ruther, D. Komitowski, FR Schubert, EF Wagner, Oncogenie 4, 861 ... Cited by 670 - Related articles - BL Direct - All 10 versions Bioorganic mechanisms TC Bruice, SJ Benkovic - 1966 - WA Benjamin Cited by 564 - Related articles - Find in ChinaCat - All 2 versions Catalytic antibodies , K Janda, AD Napper, SJ Benkovic , RA - Cold Spring Harbor , 1987 - symposium.cshlp.org ... A. TRAMONTANO,* K. JANDA,* AD NAPPER, t SJ BENKOVIC , t AND RA LERNER* ~Department of Molecular Biology, Research Institute of Scripps Clinic ... There are about 10 s primary binding specificities in the im- munologic ~1 repertoire, and each can generate, by a process ... Cited by 404 - Related articles - All 10 versions Colloidal Au-enhanced surface plasmon resonance for ultrasensitive detection of DNA hybridization , FG Salinas, SJ Benkovic , MJ Natan, CD - J. Am. Chem. , 2000 - ACS Publications ... Lin He, Michael D. Musick, Sheila R. Nicewarner, Frank G. Salinas, Stephen J. Benkovic , Michael J. Natan, and Christine D. Keating*. ... Metal deposition occurred at a pressure of 2 10 - 6 mbar at 0.2 nm/ s , monitored by an internal QCM. ... Cited by 404 - Related articles - BL Direct - All 4 versions At the crossroads of chemistry and immunology: catalytic antibodies RA Lerner, SJ Benkovic , PG Schultz - Science, 1991 - sciencemag.org ... variable amino acids. R Hirschmann, A. Smith 3rd, C. Taylor, P. Benkovic , S . Taylor, K. Yager, P. Sprengeler, and S . Benkovic (1994) Science 265, 234-237 | Abstract | PDF Expanding the scope of RNA catalysis. Prudent JR ... Cited by 428 - Related articles - All 7 versions A perspective on enzyme catalysis SJ Benkovic , S Hammes-Schiffer - Science, 2003 - sciencemag.org The seminal hypotheses proposed over the years for enzymatic catalysis are scrutinized. The historical record is explored from both biochemical and theoretical perspectives. Particular attention is given to the impact of molecular motions within the protein on the enzyme's catalytic ... Cited by 359 - Related articles - All 9 versions Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces from pnas.org , CF Barbas, KD Janda, SJ Benkovic , - Proceedings of the , 1991 - National Acad Sciences ANGRAY S . KANG*, CARLOS F. BARBAS*, KIM D. JANDA*, STEPHEN J. BENKOVICt, AND RICHARD A. LERNER** *Departments of Molecular Biology and Chemistry, The Scripps Research Institute, 10666 North Torrey Pines Road, La Jolla, CA 92037; and tDepartment of ... Cited by 303 - Related articles - All 11 versions Mechanism of DNA strand transfer reactions catalyzed by HIV-1 reverse transcriptase JA Peliska, SJ Benkovic - Science, 1992 - sciencemag.org ... articles. Mechanism of DNA strand transfer reactions catalyzed by HIV-1 reverse transcriptase. JA Peliska and SJ Benkovic Department of Chemistry, Pennsylvania State University, University Park 16802. ... Y. Li and S . Carpenter (2001) J. Gen. Virol. ... Cited by 258 - Related articles - BL Direct - All 5 versions V Mizrahi, PA Benkovic , KA Johnson, SJ Benkovic - Biochemistry, 1987 - ACS Publications ... complexes. The rate is not limited by the actual polymerization but by a separate step, possibly important in ensuring fidelity Network of coupled promoting motions in enzyme catalysis from pnas.org , PT Rajagopalan, SJ Benkovic , S - Proceedings of the , 2002 - National Acad Sciences A network of coupled promoting motions in the enzyme dihydrofolate reductase is identified and characterized. The present identification is based on genomic analysis for sequence conservation, kinetic measurements of multiple mutations, and mixed quantum/classical ... Cited by 190 - Related articles - BL Direct - All 17 versions A combinatorial approach to hybrid enzymes independent of DNA homology from 128.111.114.12 M Ostermeier, JH Shim, SJ Benkovic - Nat. Biotechnol, 1999 - 128.111.114.12 ... Marc Ostermeier, Jae Hoon Shim, and Stephen J. Benkovic * ... ITCHY A IT-A18 1100 G 101203 2 (1) IT-A8 1113 114203 9 (4) IT-A5 1129 130203 3 (1) IT-A26 1136 137203 1 (1) IT-A3 1140 141203 3 (1) IT-A1 1144 145203 3 (1) IT-A12 1144 S 146203 ... Cited by 202 - Related articles - View as HTML - All 13 versions Construction and evaluation of the kinetic scheme associated with dihydrofolate reductase from Escherichia coli CA Fierke, KA Johnson, SJ Benkovic - Biochemistry, 1987 - ACS Publications ... 4b H2F 0.5 f 0.3 0.22 f 0.06 H4F 0.06 f 0.01 0.1 f 0.01b K. Taira and S . J. Benkovic . Dersonal communication. bThis DaDer. served with the inhibitor methotrexate (Williams et al., 1979; Blakley Cocco, 1985b). Second, the ... Cited by 198 - Related articles - All 6 versions R EPLISOME-M EDIATED DNA R EPLICATION from uprm.edu SJ Benkovic , AM Valentine, F - Annual Review of , 2001 - annualreviews.org ... 70: 181-208 (Volume publication date July 2001). Stephen J. Benkovic , Ann M. Valentine, and Frank Salinas. Department of Chemistry ... helicase, T7 polymerase catalyzes the polymerization of tens of thousands of nucleotides at a rate of approximately 300 nucleotides s 1 . The ... Cited by 170 - Related articles - BL Direct - All 5 versions Kinetic mechanism whereby DNA polymerase I (Klenow) replicates DNA with high fidelity RD Kuchta, P Benkovic , SJ Benkovic - Biochemistry, 1988 - ACS Publications ... Robert D. Kuchta, Patricia Benkovic , and Stephen J. Benkovic * ... The template base( s ) to be copied along with the 3'-ter- minal base( s ) of the primer/template is (are) included in the text after each DNA . bThe labels containing a letter have a mismatched primer ... Cited by 162 - Related articles - All 3 versions Production of cyclic peptides and proteins in vivo from pnas.org , M Wall, DC Wahnon, SJ Benkovic - Proceedings of the , 1999 - National Acad Sciences ... Contributed by Stephen J. Benkovic . ... An expressed fusion protein (F) folds to form an active protein ligase (1). The enzyme catalyzes an N-to- S acyl shift (2) at the targetI N junction to produce a thioester intermediate (T), which undergoes transesterification (3) with a side-chain ... Cited by 155 - Related articles - BL Direct - All 11 versions Metallo- -lactamase: structure and mechanism Z Wang, W Fast, AM Valentine, SJ Benkovic - Current Opinion in chemical , 1999 - Elsevier ... Zhigang Wang, Walter Fast, Ann M Valentine and Stephen J Benkovic E-mail The Corresponding Author. ... The S . maltophilia L1 enzyme shares the lowest sequence identity with the other known metallo--lactamases and belongs to class Bc. ... Cited by 152 - Related articles - BL Direct - All 4 versions Relating protein motion to catalysis S Hammes-Schiffer, SJ Benkovic - Biochemistry, 2006 - annualreviews.org Abstract This review examines the linkage between protein conformational motions and enzyme catalysis. The fundamental issues related to this linkage are probed in the context of two enzymes that catalyze hydride transfer, namely dihydrofolate reductase and liver alcohol ... Cited by 147 - Related articles - BL Direct - All 3 versions Mice deficient in TNF receptors are protected against dopaminergic neurotoxicity: implications for Parkinson's disease from fasebj.org K Sriram, JM Matheson, SA Benkovic , DB Miller, MI - The FASEB Journal, 2002 - FASEB ... disease Krishnan Sriram, Joanna M. Matheson, Stanley A. Benkovic , Diane B. Miller, Michael I. Luster and James P. O'Callaghan ... Key words: brain neurodegeneration neuroprotection MPTP T he neuropathological basis of Parkinson' s disease (PD) involves a progressive ... Cited by 150 - Related articles - BL Direct - All 5 versions Kinetic mechanism of DNA polymerase I (Klenow fragment): identification of a second conformational change and evaluation of the internal equilibrium constant ME Dahlberg, SJ Benkovic - Biochemistry, 1991 - ACS Publications ... and KF-DNA,+l-PPi complexes was not observed in correct incorporation 14/20-mer as described earlier (Dahlberg Benkovic , 1991). ... Cited by 142 - Related articles - BL Direct - All 3 versions Cloning of the immunological repertoire in Escherichia coli for generation of monoclonal catalytic antibodies: construction of a heavy chain variable region-specific from pnas.org , BN Hay, KD Janda, SJ Benkovic , RA - Proceedings of the , 1989 - National Acad Sciences ... (catalytic antibodies/variable gene repertoire/antigen bhding) L. SASTRY*, M. ALTING-MEESt, W. D. HUSEt, J. M. SHORTt, J. A. SORGE*t, BN HAY*, KD JANDA*, SJ BENKOVIC *t, AND R. A. LERNER* ... 36, 1-44. 2. Lerner, R. A. Benkovic , SJ (1988) BioEssays 9, 107-112. ... Cited by 136 - Related articles - All 9 versions Chemical basis for enzyme catalysis from ucsb.edu TC Bruice, SJ Benkovic - Biochemistry, 2000 - ACS Publications ... Correlative motions appear to participate in the formation of NACs in the catechol O-methyltransferase E S complex and in the rate of turnover of dihydrofolate reduction. ... 5. Cannon, WR, and Benkovic , SJ (1998) J. Biol. Chem. 273, 2625726250. ... Cited by 137 - Related articles - BL Direct - All 8 versions Human immunodeficiency virus type 1 reverse transcriptase: spatial and temporal relationship between the polymerase and RNase H activities from pnas.org , JA Peliska, SJ Benkovic - Proceedings of the , 1992 - National Acad Sciences ... Department of Chemistry, The Pennsylvania State University, 152 Davey Laboratory, University Park, PA 16802 Contributed by Stephen J. Benkovic , July 20 ... was 50 1.l; aliquots (5 Al) were removed from the reaction mixture at each time point and quenched by addition of S Al of ... Cited by 132 - Related articles - BL Direct - All 7 versions DNA Polymerase Fidelity: Kinetics, Structure, and Checkpoints CM Joyce, SJ Benkovic - Biochemistry, 2004 - ACS Publications ... S ., Sawaya, MR, and Ellenberger, T. (1999) An open and closed case for all polymerases, Structure7, R31R35. ... 17. Dahlberg, ME, and Benkovic , SJ (1991) Kinetic mechanism of DNA polymerase I (Klenow fragment): identification of a second conformational change and ev. Cited by 133 - Related articles - BL Direct - All 4 versions Induction of an antibody that catalyzes the hydrolysis of an amide bond. KD Janda, D Schloeder, SJ Benkovic , RA - Science (New York, , 1988 - ncbi.nlm.nih.gov 1. Science. 1988 Sep 2;241(4870):1188-91. Induction of an antibody that catalyzes the hydrolysis of an amide bond. Janda KD, Schloeder D, Benkovic SJ, Lerner RA. Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037. ... Cited by 142 - Related articles - All 6 versions Creating multiple-crossover DNA libraries independent of sequence identity from pnas.org GL Moore, CD Maranas, SJ Benkovic - Proceedings of the , 2001 - National Acad Sciences We have developed, experimentally implemented, and modeled in silico a methodology named SCRATCHY that enables the combinatorial engineering of target proteins, independent of sequence identity. The approach combines two methods for recombining genes: incremental ... Cited by 124 - Related articles - BL Direct - All 21 versions Ferritin, transferrin, and iron in selected regions of the adult and aged rat brain SA Benkovic , JR Connor - The Journal of Comparative , 1993 - Wiley Online Library ... O 1993 WILEY-LISS, INC. Page 2. 98 SA BENKOVIC AND JR CONNOR al., '87). ... Scale bars = 10 pm in A, B, DF; 20 pm in C. Page 3. Page 4. 100 TABLE 1 Summary of Cellular Distribution of Ferritin, Transfernn, and Iron in the Rat Brain' SA BENKOVIC AND JR CONNOR ... Cited by 123 - Related articles - BL Direct - All 4 versions Backbone Dynamics in Dihydrofolate Reductase Complexes: Role of Loop Flexibility in the Catalytic Mechanism Osborne, J Schnell, SJ Benkovic , HJ Dyson, PE - Biochemistry, 2001 - ACS Publications ... Michael J. Osborne, Jason Schnell, Stephen J. Benkovic , H. Jane Dyson, and Peter E. Wright* . ... 1000 s - 1 ) exhibits a deuterium isotope effect of 3, an indication that no kinetically significant reorganization of the Michaelis complex is necessary in the catalytic step (3). ... Cited by 120 - Related articles - BL Direct - All 6 versions A DNA fragment with an alpha-phosphorothioate nucleotide at one end is asymmetrically blocked from digestion by exonuclease III and can be replicated in vivo from pnas.org SD Putney, SJ Benkovic , PR - Proceedings of the , 1981 - National Acad Sciences ... Abbreviations: dATP , the Sp diastereomer of 2'-deoxyadenosine 5'-O-(1-thiotriphosphate); dAMP and dNTP are corresponding a-thio analogs; kb,kilobase( s ); bp, base pair( s ). 7350 Thepublication costs ofthis article were defrayedin partbypagecharge payment. ... Cited by 113 - Related articles - All 8 versions Iron regulation in the brain: histochemical, biochemical, and molecular considerations JR Connor, SA Benkovic - Annals of neurology, 1992 - interscience.wiley.com ... only in oligodendrocytes, but also in microglial cells in both human 1211 and rat brains ( S . A. Benkovic and JR Connor, unpublished observations). Also in rat brain (investigations in human brain are in progress), ferritin and iron are found in tanycytes that line the ventricles. ... Cited by 111 - Related articles - All 4 versions Isoforms of ferritin have a specific cellular distribution in the brain Connor, KL Boeshore, SA Benkovic , - Journal of , 1994 - interscience.wiley.com ... Connor JR, Benkovic SA (1992): Iron regulation in the brain: Histo- chemical, biochemical and molecular considerations. Ann Neurol 32:S51-S61. Connor JR, Menzies SL, St. Martin S , Mufson EJ (1991): The cellular distribution of transfemn, femtin, and iron in the human hrdin. ... Cited by 95 - Related articles - BL Direct - All 5 versions Combinatorial protein engineering by incremental truncation from pnas.org , AE Nixon, JH Shim, SJ Benkovic - Proceedings of the , 1999 - National Acad Sciences ... Contributed by Stephen J. Benkovic . ... Building on classic examples of protein fragment complementation, such as ribonuclease S (1) and -galactosidase (2), researchers have used protein fragment complementation to examine theories of protein evolution (3), protein folding ( ... Cited by 105 - Related articles - BL Direct - All 18 versions Peptide synthesis catalyzed by an antibody containing a binding site for variable amino acids , AB Smith 3rd, CM Taylor, PA Benkovic , SD Taylor, KM - Science, 1994 - sciencemag.org ... Sci. Tech- nol. B 9, 1061 (1991). 7. J. S . Foster and JE Frommer, Nature 333, 542 (1988). ... Ralph Hirschmann,* Amos B. Smith 111,* Carol M. Taylor, Patricia A. Benkovic , Scott D. Taylor, Kraig M. Yager, Paul A. Sprengeler, Stephen J. Benkovic * ... Cited by 91 - Related articles - BL Direct - All 9 versions A fluorescence-based assay for monitoring helicase activity from pnas.org , LC Sowers, DP Millar, SJ Benkovic - Proceedings of the , 1994 - National Acad Sciences ... Duarte, CA 91010; and $Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037 Contributed by Stephen J. Benkovic , March 30 ... Aliquots (10 1d) of the unwinding reaction mixture were taken at 10- s intervals and added to 10 Adof quench solution. ... Cited by 96 - Related articles - BL Direct - All 10 versions Dynamics of a flexible loop in dihydrofolate reductase from Escherichia coli and its implication for catalysis CJ Falzone, PE Wright, SJ Benkovic - Biochemistry, 1994 - ACS Publications ... This exchange process was altered (either removed or made fast on the NMR time scale) by deleting three hairpin turn forming residues from the loop and filling the gap with a singleglycine from jbc.org , ME Dahlberg, SJ Benkovic , ND Grindley, CM - Journal of Biological , 1992 - ASBMB ... duplex, the 13/ 20-mer, whose preparation, purification, and quantitation have been described previously (Kuchta et al., 1987; Dahlberg and Benkovic , 1991). ... mixing were 10, 30, and 50 p ~ . The reaction was quenched with 0.1 M EDTA at time points between 0 and 5 s and the ... Cited by 102 - Related articles - All 4 versions A mass spectrometric solution to the address problem of combinatorial libraries from psu.edu CL Brummel, INW Lee, Y Zhou, SJ Benkovic , N - Science, 1994 - nxw.chem.psu.edu ... R Milberg, and S . Mullen for helpful discussions, 5 November 1993; accepted 23 February 1994 A Mass Spectrometric Solution to the Address Problem of Combinatorial Libraries Christopher L Brummel, Irene NW Lee, Ying Zhou, Stephen J. Benkovic ,* Nicholas Winograd* The ... Cited by 97 - Related articles - BL Direct - All 9 versions The nucleotide analog 2-aminopurine as a spectroscopic probe of nucleotide incorporation by the Klenow fragment of Escherichia coli polymerase I and MW Frey, LC Sowers, DP Millar, SJ Benkovic - Biochemistry, 1995 - ACS Publications ... Previous studies with Klenow fragment from ucsc.edu DD Cox, SJ Benkovic , LM Bloom, FC - Journal of the , 1988 - ACS Publications ... DD Cox,+ S . J. Benkovic ,$ LM Bloom,' FC Bradley,+ MJ Nelson,$ L. Que, Jr.,*+ and DE Wallickt Contribution from the Department of Chemistr , University of Minnesota, Minneapolis. ... (9) Wallick, DE; Bloom, L. M.; Gaffney, BJ; Benkovic , S . J. Bio- chemistry 1984, 23, 1295-1302. ... Cited by 85 - Related articles - All 3 versions Transition-state stabilization as a measure of the efficiency of antibody catalysis JD Stewart, SJ Benkovic - 1995 - nature.com ... | ChemPort |. 12. Stewart, JD, Roberts, VA, Thomas, N., Getzoff, ED Benkovic , SJ Biochemistry 33, 19912003 (1994). 13. Posner, B., Smiley, J., Lee, I. Benkovic , S . Trends biochem. Sci. 19, 145150 (1994). | Article | PubMed | ISI | ChemPort |. 14. Stewart, JD et al. Proc. natn ... Cited by 69 - Related articles - BL Direct - All 6 versions Antibody remodeling: a general solution to the design of a metal-coordination site in an antibody binding pocket from pnas.org , BL Iverson, SA Iverson, SJ Benkovic , - Proceedings of the , 1990 - National Acad Sciences ... Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037; and tDepartment of Chemistry, Pennsylvania State University, University Park, PA 16802 Contributed by Stephen J. Benkovic , June 1, 1990 ... Cited by 80 - Related articles - All 9 versions Evidence for a Functional Role of the Dynamics of Glycine-121 of Escherichia coli Dihydrofolate Reductase Obtained from Kinetic Analysis of a Site-Directed Mutant CE Cameron, SJ Benkovic - Biochemistry, 1997 - ACS Publications ... 19 from the catalytic center of the enzyme has large-amplitude backbone motions on the nanosecond time scale [Epstein, DM, Benkovic , SJ, and ... Single-turnover experiments indicated that hydride transfer was reduced by 200-fold to a rate of 1.3 s - 1 and was the rate-limiting ... Cited by 82 - Related articles - BL Direct - All 5 versions
Stephen J Benkovic Department of Chemistry, The Pennsylvania State University, USA Head of Section: Chemical Biology Biocatalysis 部分名著与科研绩效： http://scholar.google.com/scholar?start=0q=Stephen+J+Benkovichl=enas_sdt=2000 A perspective on enzyme catalysis SJ Benkovic , S Hammes-Schiffer - Science, 2003 - sciencemag.org The seminal hypotheses proposed over the years for enzymatic catalysis are scrutinized. The historical record is explored from both biochemical and theoretical perspectives. Particular attention is given to the impact of molecular motions within the protein on the enzyme's catalytic ... Cited by 341 - Related articles - All 10 versions 科学杂志检索结果： http://www.sciencemag.org/cgi/search?session_query_ref=rbs.queryref_1279668350094COLLECTIONS=hw1JC=sciFULLTEXT=%28Stephen+AND+J+AND+Benkovic%29FULLTEXTFIELD=lemcontentTITLEABSTRACTFIELD=lemhwcomptitleabsRESOURCETYPE=HWCITABSTRACTFIELD=lemhwcompabstractTITLEFIELD=lemhwcomptitle Science Magazine Search Results Results 1 to 10 of 64 found
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