miR-181a是否能调节 白血病耐药细胞株K562/A02对道诺霉素(又称柔红霉素，daunorubicin)的敏感性，以及这种调节作用的内在机制如何？本研究发现白血病耐药细胞株K562/A02中的miR-181a水平要比白血病细胞株K562中的低。 同时K562转染了miR-181a抑制剂后细胞存活率上升；而K562/A02转染miR-181a类似物之后细胞存活率下降。而且miR-181a可以增强道诺霉素诱导的K562/A02细胞凋亡；而转染了BCL-2 siRNA的K562/A02细胞存活率下降。由此可见， miR-181a在K562/A02细胞的道诺霉素耐药性发展中起到一定的作用，而这种作用可能是通过BCL-2介导实现的。 图例: 白血病细胞株K562及其耐药株K562/02中miR-181a 和BCL2的相关性 miR-181a sensitizes a multidrug-resistant leukemia cell line K562/A02 to daunorubicin by targeting BCL-2 Hao Li, Lulu Hui, and Wenlin Xu Department of Central Laboratory, The Affiliated People's Hospital, Jiangsu University, Zhenjiang, China. The aim of this study was to investigate whether miR-181a could modulate the sensitivity of the leukemia drug-resistant cell line K562/A02 to the chemotherapeutic agent daunorubicin (DNR), and explore the mechanism of miR-181a on the DNR sensitivity of K562/A02 cells. MicroRNA microarray and stem-loop reverse transcription-polymerase chain reaction were used to detect the expression of miR-181a. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay was performed to quantify the effect of miR-181a on K562 cells growth and viability. Apoptotic cells were quantitatively detected using Annexin V/FITC and PI apoptosis detection kit. BCL-2 protein expression was measured by western blot. Luciferase reporter vector with the putative BCL-2 3' untranslated region was constructed to explore whether BCL-2 was a direct target gene of miR-181a. BCL-2 siRNA was transfected into the cell to explore the relationship between BCL-2 and DNR resistance. The miR-181a expression level was lower in the K562/A02 cells than in the K562 cells (P 0.05). K562 cells that were transfected with miR-181a inhibitor had a significantly higher survival than K562 cells, and K562/A02 cells that were transfected with the miR-181a mimic had a significantly lower survival than K562/A02 cells (P 0.05). miR-181a could enhance DNR-induced apoptosis in K562/A02 cells. BCL-2 siRNA transfected K562/A02 cells had decreased survival compared with the K562/A02 control group. In conclusion, miR-181a could play a role in the development of DNR resistance in K562/A02 cells and the over-expression of miR-181a could sensitize K562/A02 cells to DNR by targeting BCL-2. Acta Biochim Biophys Sin (Shanghai). 2012 Mar;44(3):269-77. doi: 10.1093/abbs/gmr128. 全文： http://abbs.oxfordjournals.org/content/44/3/269.full.pdf+html
http://arrowsmith.psych.uic.edu/cgi-bin/arrowsmith_uic/edit_b.cgi Start A-Literature C-Literature B-list Filter Literature A-query: P53 and Bcl-2 C-query: apoptosis and family proteins The B-list contains title words and phrases (terms) that appeared in both the A and the C literature. 821 articles appeared in both literatures and were not included in the process of computing the B-list but can be viewed here . The results of this search are saved under id # 18279 and can be accessed from the start page after you leave this session. There are 3918 terms on the current B-list ( 2014 are predicted to be relevant), which is shown ranked according to predicted relevance. The list can be further trimmed down using the filters listed in the left margin. 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Use Ctrl to select multiple B-terms. job id # 18279 started Wed Jan 5 22:44:10 2011 Max_citations: 50000 Stoplist: /var/www/html/arrowsmith_uic/data/stopwords_pubmed Ngram_max: 3 18279 Search ARROWSMITH A A_query_raw: P53 and Bcl-2Wed Jan 5 22:45:51 2011 A query = P53 and Bcl-2 started Wed Jan 5 22:45:52 2011 A query resulted in 7207 titles 18279 Search ARROWSMITH C C_query_raw: apoptosis and family proteins Wed Jan 5 22:46:55 2011 C: apoptosis and family proteins 16267 A: pubmed_query_A 7207 AC: ( P53 and Bcl-2 ) AND ( apoptosis and family proteins ) 821 C query = apoptosis and family proteins started Wed Jan 5 22:46:56 2011 C query resulted in 16267 titles A AND C query resulted in 821 titles 15853 B-terms ready on Wed Jan 5 22:49:03 2011 Sem_filter: Concepts Ideas 3918 B-terms left after filter executed Wed Jan 5 23:17:25 2011 B-list on Wed Jan 5 23:56:59 2011 1 mitochondrial permeability transition 2 caspase activation 3 jun n terminal 4 factor beta signaling 5 neural stem cell 6 cytochrome c release 7 expression bcl-2 bax 8 zinc finger 9 c jun n 10 homeodomain 11 c release caspase 12 release caspase activation 13 bax protein expression 14 cisplatin resistant 15 value p53 16 protein bim 17 vascular endothelial growth 18 beta signaling 19 apoptosis mitochondrial pathway 20 bcl-2 bax expression 21 molecular mechanism apoptosis 22 bax expression 23 laryngeal squamous cell 24 signal regulated kinase 25 extracellular signal regulated 26 apoptosis inducing factor 27 activation caspase 28 apoptosis expression bcl-2 29 hypoxia inducible 30 n terminal kinase 31 terminal kinase 32 bax alpha 33 apoptosis gastric 34 apoptosis non small 35 c jun nh2-terminal 36 death receptor mitochondrial 37 induce apoptosis mitochondrial 38 colorectal adenocarcinoma 39 bak expression 40 rat ventricular myocyte 41 apoptosis chronic lymphocytic 42 rat hepatic stellate 43 caspase-3 activation 44 apoptosis proliferation 45 expression apoptosis related 46 expression laryngeal squamous 47 hypoxia inducible factor 48 apoptosis human nasopharyngeal 49 arrest apoptosis human 50 cycle arrest apoptosis 51 arrest apoptosis 52 jak stat 53 mitochondrial bax 54 invasive ductal 55 involvement bax 56 hormone refractory prostate 57 bax activation 58 activation extracellular signal 59 myocardial apoptosis 60 value apoptosis 61 activation mitogen activated 62 activation caspase-3 63 human breast epithelial 64 wild p53 65 expression laryngeal 66 bcl-2 bax ratio 67 apoptosis colorectal 68 apoptosis human gastric 69 release apoptosis inducing 70 induce apoptosis gastric 71 non small cell 72 diffuse large b 73 bax ratio 74 refractory prostate cancer 75 bax expression human 76 resistant ovarian 77 regulation bax 78 expression profiling 79 bax bcl-2 ratio 80 cyclooxygenase-2 expression 81 myc expression 82 expression bax bcl-2 83 bax mitochondrial 84 bax bcl-2 expression 85 prognosis colorectal 86 invasive ductal carcinoma 87 apoptosis mitochondrial 88 mitochondrial apoptosis pathway 89 invasive ductal breast 90 beta amyloid 91 expression apoptosis regulatory 92 beta amyloid induced 93 bax mrna expression 94 bcl-2-expressing 95 involvement caspase 96 double strand break 97 activation bax 98 terminal kinase activation 99 expression bax 100 amyloid beta peptide 101 gastric carcinogenesis 102 amyloid beta 103 alternatively spliced 104 apoptotic proliferative 105 apoptosis ovarian 106 induced apoptosis gastric 107 mitochondrial permeability 108 mitochondrial apoptotic pathway 109 apoptosis modulator 110 induced apoptosis ovarian 111 mitochondrial apoptotic 112 independently bcl-2 113 proliferator activated receptor 114 cisplatin resistant ovarian 115 expression survivin 116 expression bcl-2 117 mitochondrial death pathway 118 resistant ovarian cancer 119 adenovirus mediated transfer 120 apoptosis caspase activation 121 alpha induced apoptosis 122 expression renal cell 123 mesenchymal stem cell 124 differentiation apoptosis 125 invasive breast carcinoma 126 chemotherapy induced apoptosis 127 apoptosis vascular smooth 128 transducer activator transcription 129 chemoradiation therapy 130 signal transducer activator 131 neuronal apoptosis 132 c myc expression 133 mitochondrial release cytochrome 134 hormone refractory 135 laryngeal squamous 136 stat pathway 137 expression p53 138 p53 expression 139 extracellular signal 140 mitochondrial apoptosis 141 apoptosis human myeloid 142 h pylori 143 c elegans 144 alternative splicing 145 expression apoptosis regulating 146 apoptosis human malignant 147 invasive breast 148 diffuse large cell 149 p53-induced apoptosis 150 apoptosis oral 151 activation akt 152 c ebp 153 inducing signaling complex 154 release apoptosis 155 caspase-9 activation 156 ventricular myocyte 157 keratinocyte growth 158 apoptosis vascular 159 increase bax 160 n 4-hydroxyphenyl retinamide 161 effect bax 162 induced neuronal apoptosis 163 endometrial cancer cell 164 kappab activation 165 proliferation apoptosis 166 cancer gene therapy 167 human mesenchymal stem 168 jun nh terminal 169 beta cell apoptosis 170 diffuse large 171 homeodomain interacting protein 172 apoptosis jurkat t 173 expression endometrial carcinoma 174 inducible factor 175 neoadjuvant chemotherapy 176 bcl-2 protein expression 177 mitochondrial signaling 178 apoptosis expression 179 bcl-2 expression 180 breast ovarian cancer 181 nf kappab activation 182 neuronal death 183 expression apoptosis 184 expression analysis 185 small cell lung 186 significance bcl-2 187 atm dependent 188 id-1 189 apoptosis differentiation 190 box binding 191 element binding 192 resistance ovarian 193 large b cell 194 expression colorectal carcinoma 195 damage induced apoptosis 196 apoptosis multiple 197 neuronal apoptosis induced 198 expression caspase-3 199 mitochondrial dysfunction apoptosis 200 anaplastic large cell 201 apoptosis cultured 202 prognostic significance bcl-2 203 survival signaling 204 neuroprotective effect 205 akt activation 206 regulation cyclin 207 receptor status 208 ovarian surface 209 induced apoptosis colorectal 210 apoptosis bcl-2 expression 211 bad phosphorylation 212 tumor xenograft 213 apoptosis colonic 214 overexpression bax 215 survival signal 216 apoptosis regulatory 217 proliferation apoptosis human 218 modulation bcl-2 219 prognostic value apoptosis 220 positive breast 221 predictive value p53 222 inhibited bcl-2 223 role hypoxia inducible 224 growth induce apoptosis 225 intraductal papillary mucinous 226 expression profile 227 g2 m arrest 228 survivin expression 229 c myc 230 value bcl-2 231 carcinoma xenograft 232 activation caspase-8 233 pro survival 234 erbb-2 expression 235 proliferation induce apoptosis 236 based chemotherapy 237 cancer xenograft 238 classical hodgkin lymphoma 239 jurkat t 240 apoptosis reactive oxygen 241 expression bcl xl 242 effector caspase 243 expression colorectal 244 interferon regulatory factor 245 expression caspase 246 apoptosis mammary 247 apoptosis human cervical 248 protein bad 249 mitochondrial membrane potential 250 primary effusion lymphoma 251 invasive urothelial 252 immature thymocyte 253 nf kappa 254 p63 expression 255 mcl-1 expression 256 alternatively 257 focal cerebral ischemia 258 mammary epithelial cell 259 overexpression bcl-2 260 apoptosis expression apoptosis 261 beta1-induced apoptosis 262 activation nf kappab 263 e2f1-induced 264 cutaneous t cell 265 apoptosis cultured human 266 muscle invasive 267 expression head neck 268 jnk activation 269 human colorectal 270 regulation bcl-2 271 apoptosis neuronal 272 regulation mcl-1 273 significance p53 274 latent membrane 275 mediated caspase activation 276 survival cell death 277 nf kappab signaling 278 cell cycle arrest 279 marker colorectal 280 cd34 hematopoietic 281 hepatoma cell line 282 proliferation estrogen 283 expression estrogen 284 cancer prognostic 285 adenoviral vector 286 apoptosis ventricular 287 c myb 288 epithelial apoptosis 289 effect paclitaxel 290 oncogene expression 291 expression caspase activation 292 locally advanced 293 gastric epithelial cell 294 caspase activation human 295 activation bcl-2 296 locally advanced breast 297 expression bcl 298 immunohistochemical expression bcl-2 299 e2f1-induced apoptosis 300 oral squamous http://arrowsmith.psych.uic.edu/cgi-bin/arrowsmith_uic/show_sentences.cgi Start A-Literature C-Literature B-list Filter Literature AB literature B-term BC literature P53 and Bcl-2 zinc finger apoptosis and family protei... 1: KRAB-type zinc-finger protein Apak specifically regulates p53-dependent apoptosis.2009 Add to clipboard 2: Selective transcription of p53 target genes by zinc finger -p53 DNA binding domain chimeras.2004 Add to clipboard 3: The p53-activated gene, PAG608, requires a zinc finger domain for nuclear localization and oxidative stress-induced apoptosis.2002 Add to clipboard 1: Structural determinants of specific DNA-recognition by the THAP zinc finger .2010 Add to clipboard 2: The thap -zinc finger protein thap1 associates with coactivator HCF-1 and O-GLcNAc transferase: A link between DYT6 and DYT3 dystonias.2010 Add to clipboard 3: ZNF424, a novel human KRAB/C2H2 zinc finger protein, suppresses NFAT and p21 pathway.2010 Add to clipboard 4: Zinc-finger transcription factor slug contributes to the survival advantage of chronic myeloid leukemia cells.2010 Add to clipboard 5: 2010 Add to clipboard 6: Structure-function analysis of the THAP zinc finger of THAP1, a large C2CH DNA-binding module linked to Rb/E2F pathways.2008 Add to clipboard 7: Knockdown of Snail, a novel zinc finger transcription factor, via RNA interference increases A549 cell sensitivity to cisplatin via JNK/mitochondrial pathway.2008 Add to clipboard 8: Zinc-finger transcription factor snail accelerates survival, migration and expression of matrix metalloproteinase-2 in human bone mesenchymal stem cells.2007 Add to clipboard 9: Activation of transcriptional activities of AP1 and SRE by a novel zinc finger protein ZNF445.2006 Add to clipboard 10: Identification and characterization of the human SCAN domain zinc-finger gene ZNF449.2006 Add to clipboard 11: 2006 Add to clipboard 12: The zinc-finger transcription factor Snail downregulates proliferating cell nuclear antigen expression in colorectal carcinoma cells.2005 Add to clipboard 13: The promyelocytic leukemia zinc finger protein down-regulates apoptosis and expression of the proapoptotic BID protein in lymphocytes.2004 Add to clipboard 14: Chorioallantoic fusion defects and embryonic lethality resulting from disruption of Zfp36L1, a gene encoding a CCCH tandem zinc finger protein of the Tristetraprolin family.2004 Add to clipboard 15: Transcriptional activities of the zinc finger protein Zac are differentially controlled by DNA binding.2003 Add to clipboard 16: Systematic characterization of the zinc-finger -containing proteins in the mouse transcriptome.2003 Add to clipboard 17: KRAB-containing zinc-finger repressor proteins.2003 Add to clipboard 18: The effects of the Fanconi anemia zinc finger (FAZF) on cell cycle, apoptosis, and proliferation are differentiation stage-specific.2002 Add to clipboard 19: POZ domain transcription factor, FBI-1, represses transcription of ADH5/FDH by interacting with the zinc finger and interfering with DNA binding activity of Sp1.2002 Add to clipboard 20: ZAS: C2H2 zinc finger proteins involved in growth and development.2002 Add to clipboard 21: Erythroid expansion mediated by the Gfi-1B zinc finger protein: role in normal hematopoiesis.2002 Add to clipboard 22: Slug, a highly conserved zinc finger transcriptional repressor, protects hematopoietic progenitor cells from radiation-induced apoptosis in vivo.2002 Add to clipboard 23: A novel missense mutation (C622G) in the zinc-finger domain of the human hairless gene associated with congenital atrichia with papular lesions.2000 Add to clipboard 24: The zinc finger protein A20 interacts with a novel anti-apoptotic protein which is cleaved by specific caspases.1999 Add to clipboard 25: SLUG, a ces-1-related zinc finger transcription factor gene with antiapoptotic activity, is a downstream target of the E2A-HLF oncoprotein.1999 Add to clipboard 26: The zinc finger protein NRIF interacts with the neurotrophin receptor p75(NTR) and participates in programmed cell death.1999 Add to clipboard 27: Molecular cloning and characterization of TIEG2 reveals a new subfamily of transforming growth factor-beta-inducible Sp1-like zinc finger -encoding genes involved in the regulation of cell growth.1998 Add to clipboard 28: Overexpression of the TGFbeta-regulated zinc finger encoding gene, TIEG, induces apoptosis in pancreatic epithelial cells.1997 Add to clipboard
标签: Bcl-2
