# 编者信息 熊荣川 明湖实验室 xiongrongchuan@126.com http://blog.sciencenet.cn/u/Bearjazz Although DNA barcoding has been widely used to identify plant species composition in temperate and tropical ecosystems, relatively few studies have used DNA barcodes to document both herbaceous and woody components of forest plot. A total of 201 species (72 woody species and 129 herbaceous species) representing 135 genera distributed across 64 families of seed plants were collected in a 25 ha CForBio subalpine forest dynamics plot. In total, 491 specimens were screened for three DNA regions of the chloroplast genome (rbcL, matK, and trnH-psbA) as well as the internal transcribed spacers (ITS) of nuclear ribosomal DNA. We quantified species resolution for each barcode separately or in combination using a ML tree-based method. Amplification and sequencing success were highest for rbcL, followed by trnH-psbA, which performed better than ITS and matK. The rbcL + ITS barcode had slightly higher species resolution rates (88.60%) compared with rbcL + matK (86.60%) and rbcL + trnH-psbA (86.01%). The addition of trnH-psbA or ITS to the rbcL + matK barcode only marginally increased species resolution rates, although in combination the four barcodes had the highest discriminatory power (90.21%). The situations where DNA barcodes did not discriminate among species were typically associated with higher numbers of co-occurring con-generic species. In addition, herbaceous species were much better resolved than woody species. Our study represents one of the first applications of DNA barcodes in a subalpine forest dynamics plot and contributes to our understanding of patterns of genetic divergence among woody and herbaceous plant species. 虽然 DNA 条形码已被广泛应用于温带和热带生态系统中植物物种的组成鉴定，但很少有研究使用 DNA 条形码来记录林地的草本植物和木本植物成分。在中国森林生物多样性监测网络中，我们选择了一个 25 公顷的亚高山森林动态图，在该图对应的区域内，共采集 64 科， 135 属， 201 种种子植物（ 72 种木本植物， 129 种草本植物）。筛选了 491 个叶绿体基因组的 3 个 DNA 区域（ rbcl 、 matK 和 trnH-psbA ）和细胞核核糖体 DNA 的转录间隔区（ ITS ）。我们使用基于 ML 树的方法分别或组合量化每个条形码的物种分辨率。 rbcL 的扩增和测序成功率最高，其次是 trnH-psbA ，其表现优于 ITS 和 matK 。与 rbcL + matK （ 86.60% ）和 rbcL + trnH-psbA （ 86.01% ）相比， rbcL + ITS 的物种分辨率略高（ 88.60% ）。在 rbcL + matK 组合的基础上添加 trnH-psbA 或 ITS 只会略微提高物种的分辨率，这四种条码的结合具有最高的分辨力（ 90.21% ）。 DNA 条形码在物种间没有区别的情况通常与较高的共生同属物种的数量有关。此外，草本植物比木本植物更容易分辨。我们的研究是 DNA 条形码在亚高山森林动态图中的首次应用，有助于我们理解木本和草本植物物种之间的遗传分化模式。 Shao-Lin T , Ya-Huang L , Hollingsworth P M , et al. DNA barcoding herbaceous and woody plant species at a subalpine forest dynamics plot in Southwest China . Ecology and Evolution, 2018.
标签: 草本
