A cotton gene encoding a plasma membrane aquaporin is involved in seedling development and in response to drought stress Jie Zhang, Dengdi Li, Dan Zou, Fang Luo, Xiulan Wang, Yong Zheng and Xuebao Li Acta Biochim Biophys Sin 2013, 45: 104–114; doi: 10.1093/abbs/gms096 Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, China Cotton (Gossypium hirsutum), the most important textile crop worldwide, often encounters abiotic stress such as drought and waterlog during its growth season (summer), and its productivity is significantly limited by adverse factors. To investigate the molecular adaptation mechanisms of this plant species to abiotic stress, a gene encoding the plasma membrane intrinsic protein (PIP) was isolated in cotton, and designated as GhPIP2;7. Quantitative reverse transcriptase polymerase chain reaction analysis indicated that GhPIP2;7 was preferentially expressed in cotyledons and leaves, and its expression was up-regulated in leaves after drought treatments. Strong expression of GUS gene driven by GhPIP2;7 promoter was detected in leaves of 5- to 10-day-old transgenic Arabidopsis seedlings, but GUS activity gradually became weak as the seedlings further developed. GhPIP2;7 promoter activity was also remarkably induced by mannitol treatment. Furthermore, yeast cells over-expressing GhPIP2;7 displayed relatively higher drought tolerance, compared with controls. Over-expression of GhPIP2;7 in Arabidopsis enhanced plant tolerance to drought stress. Collectively, these data suggested that GhPIP2;7 gene may be involved in leaf development and in response to drought stress. 图例: 甘露醇处理对水稻GhPIP2;7启动子活性的影响 全文: http://abbs.oxfordjournals.org/content/45/2/104.full 与此文相关的文献: 1 Structural mechanism of plant aquaporin gating 2 An expression analysis of a gene family encoding plasma membrane aquaporins in response to abiotic stresses in Arabidopsis thaliana 3 Overexpression of a plasma membrane aquaporin in transgenic tobacco improves plant vigor under favorable growth conditions but not under drought or salt stress 4 The role of aquaporin RWC3 in drought avoidance in rice 5 Improving plant stress tolerance and yield production: is the tonoplast aquaporin SlTIP2;2 a key to isohydric to anisohydric conversion? 6 Aquaporin expression in response to different water stress intensities and recovery in Richter-110 (Vitis sp.): relationship with ecophysiological status
可喜可贺的是其中一篇是以朱健康为通讯作者，上海植物逆境生物学研究中心为第一单位做出的工作，连接地址是 http://www.sciencemag.org/content/336/6087/1445.short 。他们报道了拟南芥中一个基因IDM1，编码乙酰化酶，影响DNA的去甲基化，把组蛋白的修饰与DNA修饰联系起来了。 另外是一项工作( http://www.sciencemag.org/content/336/6087/1448.short )是拟南芥中另外两个基因 AtMORC1 and AtMORC6。AtMORC1 and AtMORC6 are members of the conserved Microrchidia ( MORC ) adenosine triphosphatase (ATPase) family, which are predicted to catalyze alterations in chromosome superstructure. 他们工作从实验上证明了AtMORC1 and AtMORC6 是影响染色质结构，使基因表达沉默，并且在线虫中也保守（看来在进化值得做做，嘿嘿）。 另外，表观遗传现在是很火的时候，很多基因的功能根据同源性可以预测得到，比如第二篇文章。只要在实验上证明了，基本上就是一篇好文章。当然不简单是为了文章，这个是非常有意义的，因为背后的机理和机制是非常多的，一旦谁第一个做出来，知道的就是越多。在植物中，以前发现的与胁迫相关基因，转到其他物种中效果不好，也许表观遗传基因是一个重要的突破口。
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