刚开始我们本来希望做对神经干细胞的影响,可是实验进展不太顺利, 就采用本学校生化的模型进行了这个研究. 主要从内皮细胞增生角度, 从HIF-VEGF进行分析.这个研究费力但效果不理想. 将来要从肝缺血方面进行深入研究更合适. Hyperbaric oxygen preconditioning promotes angiogenesis in rat liver after partial hepatectomy Ping Ren a , 1 , ZhiMing Kang b , 1 , GuoJun Gu b , Yun Liu b , WeiGang Xu b , HengYi Tao b , John H. Zhang c , XueJun Sun b , , and Hui Ji a , , a Department of Pharmacology, China Pharmaceutical University, Nanjing, 210009, PR China b Department of Diving Medicine, Faculty of Naval Medicine, Secondary Military Medical University, Shanghai, 200433, PR China c Department of Neurosurgery, Loma Linda University, Loma Linda, California, USA Received 25 October 2007; accepted 5 June 2008. Available online 24 June 2008. Abstract Hyperbaric oxygen preconditioning (HBO-PC) increases the level of HIF-1 (hypoxia inducible factor-1) and its target gene VEGF (vascular endothelial growth factor) which is involved in angiogenesis. Liver regeneration is an angiogenesis-dependent process. We hypothesized that HIF-1 and VEGF mediated the angiogenesis effect of HBO-PC on regenerating rat liver. Male Sprague Dawley rats received HBO-PC followed by 70% partial hepatectomy. Proliferation of hepatocytes and endothelial cells was evaluated by BrdU (bromodeoxyuridine) staining. Microvascular density was assessed by immunohistochemistry. mRNA expression of HIF-1 was assessed by quantitative RT-PCR and protein levels of HIF-1 and VEGF were assessed by western blot. HIF-1 DNA-binding activity was determined with an ELISA-based kit. HBO-PC increased the proliferation index of endothelial cells and microvascular density at 48h after partial hepatectomy. The protein level and DNA-binding activity of HIF-1 and the protein level of VEGF were increased by HBO-PC before and after partial hepatectomy. Partial hepatectomy alone also increased proliferation index and the expressions of HIF-1 and VEGF. Our results indicated that the angiogenesis effect of HBO-PC on liver after partial hepatectomy could be achieved by increased HIF-1 activity and VEGF expression. However, the angiogenic effect of HBO-PC is moderate and HBO-PC failed to produce additional effect on the enhancement of HIF-1 and VEGF induced by partial hepatectomy alone. Keywords: Angiogenesis; Hyperbaric oxygen preconditioning; Hypoxia inducible factor-1; Vascular endothelial growth factor Article Outline Introduction Materials and methods Animals and study protocols Liver regeneration HBO exposure Immunohistochemistry Western blot analysis HIF-1 DNA-binding assay RNA isolation and quantitative real-time PCR Data analysis Results Wet regeneration Proliferation index BrdU Microvascular density HIF-1 VEGF Discussion Acknowledgements References 全文
标签: 高压氧
