# 编者信息 熊荣川 明湖实验室 xiongrongchuan@126.com http://blog.sciencenet.cn/u/Bearjazz Nondestructive DNA extraction was performed using a Qiagen DNeasy kit. Whole specimens were soaked overnight in extraction buffer at 37°C, with small perforations made to the side of the abdomen and DNA extracted from the supernatant. Damage to specimens (some of them to be holotypes in future species descriptions) was minimal, and softness of specimens was maintained if transferred to 70% ethanol after the extraction. Vouchers will be maintained as pinned dry specimens in the W. D. Sumlin personal collection. 使用 Qiagen DNeasy 试剂盒进行非破坏性 DNA 提取。在标本腹部侧面形成小孔,整个标本在 37 ° C 的提取缓冲液中浸泡一整夜,然后从上清液中提取 DNA 。对标本(其中一些在未来物种描述中是正模标本)的损害是最小的,如果提取后转移到 70% 的乙醇中,标本的柔软度保持不变。凭证标本将进行插针干燥,由 W.D.Sumlin 个人保管。 Pons J, Barraclough T G, Gomez-Zurita J, et al. Sequence-based species delimitation for the DNA taxonomy of undescribed insects . Systematic biology, 2006, 55(4): 595-609.