http://www.gopubmed.org/web/gopubmed/1?WEB01nbv43wsdzkq8IhI1I00f01000j10040001rl 4,960 documents semantically analyzed 1 2 Top Years Publications 2009 485 2008 428 2007 410 2005 379 2006 371 2002 327 2004 323 2001 312 2003 309 2000 254 1998 206 1999 176 1997 173 1996 134 1995 120 1994 91 1991 87 1992 83 1993 74 1989 44 1 2 1 2 3 4 Top Countries Publications USA 2,071 United Kingdom 387 Japan 339 Germany 313 China 238 France 198 Canada 134 Australia 91 Italy 89 Sweden 83 Spain 64 Netherlands 57 India 53 Denmark 49 South Korea 46 Brazil 45 Switzerland 37 Singapore 34 Taiwan 34 Belgium 29 1 2 3 4 1 2 3 ... 32 Top Cities Publications Bethesda 187 Houston 115 Tokyo 105 Cambridge 99 St. Louis 92 London 82 Seattle 81 Baltimore 77 Rockville 74 Paris 66 Boston 65 Beijing 62 New York 60 San Francisco 57 San Diego 55 Heidelberg 50 Berlin 50 Cambridge, USA 49 Shanghai 49 Atlanta 48 1 2 3 ... 32 1 2 3 ... 60 Top Journals Publications Genome Res 195 Genomics 133 Nucleic Acids Res 124 J Virol 118 P Natl Acad Sci Usa 113 Nature 111 Science 93 Gene 81 Virology 76 J Gen Virol 59 Hum Mol Genet 58 Bmc Genomics 57 J Biol Chem 52 J Clin Microbiol 47 Nat Genet 45 Am J Hum Genet 44 Bioinformatics 43 Hum Mutat 41 Plos One 41 Methods Mol Biol 39 1 2 3 ... 60 1 2 3 ... 1353 Top Authors Publications Gibbs R 43 Green E 38 Weinstock G 25 Hillier L 24 Eichler E 22 Venter J 22 Waterston R 21 Scherer S 21 Marra M 20 McPherson J 18 Lander E 18 Wilson R 16 Adams M 16 Lehrach H 16 NISC Comparative Sequencing Program 15 Mardis E 15 Ohara O 14 Rosenthal A 14 Hayashizaki Y 13 Hejtmancik J 13 1 2 3 ... 1353 1 2 3 ... 703 Top Terms Publications Genome 4,329 Genomics 4,314 Humans 3,993 Genes 3,105 Genome, Human 2,279 Base Sequence 2,154 DNA 2,136 Animals 1,796 Proteins 1,451 Sequence Analysis, DNA 1,238 Polymerase Chain Reaction 1,104 Mutation 998 Nucleotides 890 Chromosome Mapping 768 Amino Acid Sequence 768 DNA, Complementary 729 chromosome 728 Viruses 713 Mice 701 Patients 689 1 2 3 ... 703 http://www.sciencenet.cn/htmlnews/2009/11/224888.shtm 《科学》公布三个低成本完整人类基因组测序结果 来自Complete Genomics公司，华盛顿大学，哈佛医学院等处的研究人员在11月6日《科学》（Science）杂志上发表论文，描述其专利DNA测序平台，并公布了对三个完整人类基因组序列分析的结果，文章还报道了不同样本的测序成本，包括了从$8,005（87x coverage）到$1,726（45x coverage）的范围。 文章的通讯作者是来自Complete Genomics公司（CG）Radoje Drmanac博士和Dennis G. Ballinger博士。CG公司首席执行官Cliff Reid博士表示，“这项研究证明了是有可能进行精确测序，并从整个人类基因组中发现差异的”，“这种高质量，低成本的基因组测序方法能帮助研究人员对成千上百个某种疾病患者的完整基因组序列进行分析，从而获得对这种疾病的深入了解，最终找出预防和治疗的方法。” 这篇题为“Human Genome Sequencing Using Unchained Base Reads on Self-Assembling DNA Nanoarrays”的文章报道了三个人类个体的基因组测序，其中两个属于国际HapMap计划——欧洲白种男性（NA07022），西非Yoruban女性（NA19240），除此之外还有一个来自个人基因组计划的白种男性（NA20431）的成淋巴细胞DNA样品。 CG公司的这种测序技术步骤（见下图）包括：1.样品准备和文库构建；2.DNA芯片分析；3.成像，组装和分析；4.复合探针-锚定分子连接（Combinatorial probe -- anchor ligation，cPAL）。 四步测序步骤（图片来源：Complete Genomics公司） 利用这种技术，CG公司的科学家们能完成95%基因组序列的测定，并从每个基因组测序过程中识别320万-450万个变异序列。这些基因组数据库精确到1个假阳性/100kb，这是目前测序技术中可以达到的非常低的出错率。 基因组图谱和概要（图片来源：Complete Genomics公司） 人类在基因组领域面临两大挑战。其一是单纯发现可能导致特定疾病的独立或者组合基因。仅这一点就会大有用处。如果掌握了足够的数据，就能搞清同一种“疾病”实际上是一系列机能失调之和，某些已知的治疗方法会对其中这样或那种疾病产生效果，还有些疾病可能根本无法治愈。 第二大挑战是理解基因怎样通过相互作用或与其他因素互动来引发健康问题，从而在了解疾病发生发展进程的基础上，发明新的预防和治疗措施。当然，这项挑战难度更大也更有趣。从某种意义上讲，这相当于匹配词汇和理解文意之间的区别。 因此像是CG此类公司在新的市场中找到机会并不奇怪，他们的优势不仅在于能廉价排列基因组序列，还在于能提炼数据，一一列出所有基因变异。换言之，在多数研究中问题不仅围绕着整个基因组，也是围绕着任何个人基因与标准之间的相对差异。 更多阅读 《科学》发表论文摘要（英文） http://www.sciencemag.org/cgi/content/abstract/sci;1181498v1?maxtoshow=HITS=10hits=10RESULTFORMAT=fulltext=Human+Genome+Sequencing+Using+Unchained+Base+Reads+on+Self-Assembling+DNA+Nanoarrayssearchid=1FIRSTINDEX=0resourcetype=HWCIT Published Online November 5, 2009 Science DOI: 10.1126/science.1181498 Science Express Index Reports Submitted on September 3, 2009 Accepted on October 23, 2009 Human Genome Sequencing Using Unchained Base Reads on Self-Assembling DNA Nanoarrays Radoje Drmanac 1 * , Andrew B. Sparks 1 , Matthew J. Callow 1 , Aar on L. Halpern 1 , Norman L. Burns 1 , Bahram G. Kermani 1 , Paolo Carnevali 1 , Igor Nazarenko 1 , Geoffrey B. Nilsen 1 , George Yeung 1 , Fredrik Dahl 2 , Andres Fernandez 1 , Bryan Staker 1 , Krishna P. Pant 1 , J on athan Baccash 1 , Adam P. Borcherding 1 , Anushka Brownley 1 , Ryan Cedeno 1 , Linsu Chen 1 , Dan Chernikoff 1 , Alex Cheung 1 , Razvan Chirita 1 , Benjamin Curs on 1 , Jessica C. Ebert 1 , Coleen R. Hacker 1 , Robert Hartlage 1 , Brian Hauser 1 , Steve Huang 1 , Yuan Jiang 1 , Vitali Karpinchyk 1 , Mark Koenig 1 , Calvin K on g 1 , Tom Landers 1 , Catherine Le 1 , Jia Liu 1 , Celeste E. McBride 1 , Matt Morenz on i 1 , Robert E. Morey 3 , Karl Mutch 1 , Helena Perazich 1 , Kimberly Perry 1 , Brock A. Peters 1 , Joe Peters on 1 , Charit L. Pethiyagoda 1 , Kaliprasad Pothuraju 1 , Claudia Richter 1 , Abraham M. Rosenbaum 4 , Shaunak Roy 1 , Jay Shafto 1 , Uladzislau Sharanhovich 1 , Karen W. Shann on 5 , C on rad G. Sheppy 1 , Michel Sun 1 , Joseph V. Thakuria 4 , Anne Tran 1 , Dylan Vu 1 , Alexander Wait Zaranek 4 , Xiaodi Wu 6 , Snezana Drmanac 1 , Arnold R. Oliphant 1 , William C. Banyai 1 , Bruce Martin 1 , Dennis G. Ballinger 1 * , George M. Church 4 , Clifford A. Reid 1 1 Complete Genomics, Inc., 2071 Stierlin Court, Mountain View, CA 94043, USA. 2 Complete Genomics, Inc., 2071 Stierlin Court, Mountain View, CA 94043, USA.; Present address: I on Torrent Systems, San Francisco, CA, USA. 3 Complete Genomics, Inc., 2071 Stierlin Court, Mountain View, CA 94043, USA.; Present address: San Diego State University, San Diego, CA, USA. 4 Department of Genetics, Harvard Medical School, Cambridge, MA, USA. 5 Complete Genomics, Inc., 2071 Stierlin Court, Mountain View, CA 94043, USA.; Present address: Life Technologies, Carlsbad, CA, USA. 6 School of Medicine, Washingt on University, St. Louis, St. Louis, MO, USA. * To whom corresp on dence should be addressed. Radoje Drmanac , E-mail: rdrmanac@completegenomics.com !-- var u = "rdrmanac", d = "completegenomics.com"; document.getElementById("em0").innerHTML = ' ' + u + '@' + d + '\/a'//-- Dennis G. Ballinger , E-mail: dballinger@completegenomics.com !-- var u = "dballinger", d = "completegenomics.com"; document.getElementById("em1").innerHTML = ' ' + u + '@' + d + '\/a'//-- These authors c on tributed equally to this work. Genome sequencing of large numbers of individuals promises to advance the understanding, treatment, and preventi on of human diseases, am on g other applicati on s. We describe a genome sequencing platform that achieves efficient imaging and low reagent c on sumpti on with combinatorial probe anchor ligati on (cPAL) chemistry to independently assay each base from patterned nanoarrays of self-assembling DNA nanoballs (DNBs). We sequenced three human genome s with this platform, generating an average of 45- to 87-fold coverage per genome and identifying 3.2 to 4.5 milli on sequence variants per genome . Validati on of on e genome data set dem on strates a sequence accuracy of about 1 false variant per 100 kilo base s. The high-accuracy, affordable cost of $4,400 for sequencing c on sumables and scalability of this platform enable complete human genome sequencing for the detecti on of rare variants in large-scale genetic studies.
http://www.gopubmed.org/web/gopubmed/2?WEB01xszjeo5b1nueItI1I00f01000j10040001rl 检索策略：Stem Cells and animal model 对4069篇相关文献计量分析结果如下： 4,069 of 413,426 documents semantically analyzed Term: Stem Cells Description: Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells. Synonyms: Colony-Forming Unit, Colony-Forming Units, Colony Forming Unit, Colony Forming Units, Progenitor Cell, Stem Cell, Mother Cell, Progenitor Cells, Mother Cells Top Years Publications 2008 1,126 2009 1,048 2007 811 2006 706 2005 354 2004 12 2003 4 2000 3 2001 2 1999 1 1998 1 1997 1 1 2 3 Top Countries Publications USA 522 China 132 Japan 108 Germany 71 United Kingdom 63 Italy 43 France 40 Canada 33 Australia 29 Spain 24 South Korea 23 Netherlands 18 Taiwan 17 Brazil 15 Sweden 15 Israel 12 Singapore 11 Belgium 9 Switzerland 9 Turkey 9 1 2 3 1 2 3 ... 19 Top Cities Publications Boston 31 New York 28 Tokyo 26 London 24 Shanghai 17 Los Angeles 16 Beijing 16 Philadelphia 16 Houston 16 Guangzhou 15 Chicago 14 Baltimore 13 Detroit 13 Paris 13 Bethesda 13 Ann Arbor 11 Madison 11 Singapur 11 New Haven 11 Stanford 10 1 2 3 ... 19 1 2 3 ... 30 Top Journals Publications Blood 23 Cancer Res 20 Proc Natl Acad Sci U S A 19 Plos One 17 Circ Res 15 Stem Cells 14 Exp Neurol 14 Cardiovasc Res 14 Spine 14 Cell Transplant 13 J Immunol 12 J Neurosci 11 Brain Res 11 Transplantation 10 Arth Rheum/ar C Res 10 J Biol Chem 10 Gastroenterology 10 Invest Ophthalmol Vis Sci 10 Am J Physiol-heart C 10 Dis Model Mech 9 1 2 3 ... 30 1 2 3 ... 630 Top Terms Publications Animals 2,460 Mice 1,934 Fibroblasts 1,740 Stem Cells 1,444 Tissues 1,226 Models, Animal 1,186 Proteins 1,107 Genes 922 Intercellular Signaling Peptides and Proteins 834 Evaluation Studies as Topic 755 Therapeutics 734 Disease Models, Animal 610 Neoplasms 599 Patients 585 Humans 563 Wounds and Injuries 553 Adult 520 Cell Proliferation 473 Bone Marrow 470 Cells, Cultured 465 1 2 3 ... 630 1 2 3 ... 1218 Top Authors Publications Lee B 7 Orlando F 7 Chopp M 7 Cirioni O 6 Losordo D 6 Ye L 6 Asahara T 6 Vainchenker W 6 Grant M 5 Schett G 5 Jiang S 5 Rosen J 5 Gay S 5 Saika S 5 Saba V 5 Ghiselli R 5 Giacometti A 5 Mocchegiani F 5 Silvestri C 5 Scalise G 5 1 2 3 ... 1218 http://www.sciencenet.cn/htmlnews/2009/10/224503.shtm 中国科协论坛聚焦干细胞研究 ［科学时报 王学健报道］ 我国研究干细胞的动物模型，不能仅停留在小鼠等小动物上，如果能举全国之力试验大动物，如猪、猴子等，那我们就快成功了。在不久前举行的第二次中国科协论坛上，同济大学校长、中国科学院院士裴刚在谈到中国干细胞研究的机遇与挑战时，着重强调了大动物试验平台的意义。 从1999年12月干细胞研究被评为世界十大科学研究之首至今，干细胞研究已走过了10年。裴刚说：过去，干细胞研究一直比较热门，有各种原因。其一，干细胞是科学的最前沿，因为一个细胞变成很多细胞，变成个体，是怎么变的，人类永远是好奇的。其二，细胞分化以后能不能再回来？其三，如果这两种可能存在的话，有什么用？人类各种疾病是不是可以用这种方法来治疗？因此干细胞研究有非常深远的社会意义、政治意义和科学意义。 在谈到干细胞发展战略时，裴刚强调了人才和机制的重要性，希望有创新的研究体制和长期稳定的支持，开放性的招聘，长期稳定的择优支持，国际评估，开放流动。 在由裴刚担任首席科学家的第二次中国科协论坛上，会聚了南开大学校长、中国科学院院士饶子和，中国科学院动物研究所所长、中国科学院院士孟安明等20多位知名学者。论坛议题为干细胞研究领域的最新进展、重大成果和发展态势分析，我国干细胞与再生医学领域未来的发展战略和相关建议等。 为发挥中国科协所属学会在推动自主创新中的作用，根据中国科协领导关于举办高端学术论坛的指示精神，从2009年起设立中国科协论坛。论坛瞄准科学发展前沿，围绕建设创新型国家的战略目标，面向国家重大战略需求，紧密跟踪高技术发展趋势，就引领未来科技发展的关键科学问题、制约经济社会发展的瓶颈技术问题、重大工程技术中的核心科学问题，组织学术交流。 中国科协论坛强调主题前沿性、人员高端性和规模小型性，注重主题新颖，形式灵活，探讨深入。中国科协学会学术部部长沈爱民在第二次中国科协论坛上表示，希望专家学者在已经有了一些研究成果的同时，在这个论坛上，通过深入的研讨，真正产生一些观点。中国科协希望通过设立中国科协论坛的方式，提升学术交流层次，推动学科交叉和融合，营造和谐学术环境，提高学术交流的质量和成效，促进自主创新和高层次人才成长，打造新的学术交流品牌，优化学术交流格局。 《科学时报》 (2009-10-28 A1 要闻) 更多阅读 第二次科协论坛研讨干细胞研究战略
http://www.gopubmed.org/web/gopubmed/1?WEB0wq92mdaefij4I1gI1I00d000j10040001rl 1 2 Top Years Publications 2008 1,356 2004 1,355 2007 1,306 2005 1,270 2006 1,247 2003 1,236 2002 1,147 2009 1,094 2001 1,036 2000 983 1998 791 1999 773 1997 620 1996 406 1995 302 1994 261 1993 214 1992 188 1991 98 1990 77 1 2 1 2 3 4 5 Top Countries Publications USA 5,561 Japan 1,687 United Kingdom 1,222 China 1,146 Germany 798 France 634 Italy 574 Canada 463 Spain 323 South Korea 249 Netherlands 235 Australia 233 Switzerland 224 Sweden 188 Taiwan 176 Russia 143 Israel 118 India 115 Belgium 112 Austria 102 1 2 3 4 5 1 2 3 ... 47 Top Cities Publications New York 384 Tokyo 333 London 294 Houston 271 Boston 261 Baltimore 222 Paris 221 Bethesda 198 San Francisco 196 Dallas 187 Beijing 182 Seattle 171 Hiroshima 163 Rome 159 Seoul 159 Madrid 152 Berkeley 144 Philadelphia 141 Los Angeles 139 Shanghai 122 1 2 3 ... 47 1 2 3 ... 91 Top Journals Publications Mol Cell Biol 378 Nucleic Acids Res 355 P Natl Acad Sci Usa 336 J Biol Chem 292 Cancer Res 281 Oncogene 248 Genetics 217 Genomics 177 Biochem Bioph Res Co 173 Embo J 168 Cell 155 Gene Dev 150 Am J Med Genet A 147 Nature 141 Chromosoma 137 Chromosome Res 131 Int J Oncol 129 Science 129 Clin Cancer Res 128 Cytogenet Genome Res 125 1 2 3 ... 91 1 2 3 ... 1044 Top Terms Publications Telomere 11,673 Humans 10,927 chromosome, telomeric region 10,317 Telomerase 8,562 DNA 5,690 Animals 5,521 Genes 5,314 regulation of telomerase activity 4,715 telomerase activity 4,646 positive regulation of telomerase activity 4,607 Proteins 4,560 negative regulation of telomerase activity 4,556 telomerase inhibitor activity 4,460 Chromosomes 4,453 chromosome 3,804 Neoplasms 3,642 DNA-Binding Proteins 2,766 Cell Line 2,707 Base Sequence 2,685 Genome 2,418 1 2 3 ... 1044 1 2 3 ... 2615 Top Authors Publications Shay J 205 Blackburn E 133 Wright W 128 Blasco M 106 Lansdorp P 94 Greider C 89 Cech T 82 De Lange T 75 Ishikawa F 63 Gilson E 61 Kyo S 58 Reddel R 56 Tahara H 56 Gasser S 55 Ide T 55 Von Zglinicki T 53 Ohyashiki K 51 Aviv A 49 Hahn W 49 Harley C 49 1 2 3 ... 2615 见附件： Telomere or Telomerase
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