2016年高被引文章 Osteogenesis imperfecta: Clinical diagnosis, nomenclature and severity assessment American Journal of Medical Genetics Part A Arguments for the Sake of Endophenotypes: Examining Common Misconceptions About the Use of Endophenotypes in Psychiatric Genetics American Journal of Medical Genetics Part B Common marmoset as a new model animal for neuroscience research and genome editing technology Development, Growth Differentiation Clinical delineation and natural history of the PIK3CA-related overgrowth spectrum American Journal of Medical Genetics Part A The Fibroblast Growth Factor signaling pathway WIREs Developmental Biology Characterization of Human Disease Phenotypes Associated with Mutations in TREX1, RNASEH2A, RNASEH2B, RNASEH2C, SAMHD1, ADAR, and IFIH1 American Journal of Medical Genetics Part A The developmental control of size in insects WIREs Developmental Biology Derivation of Point of Departure (PoD) Estimates in Genetic Toxicology Studies and Their Potential Applications in Risk Assessment Environmental and Molecular Mutagenesis An Evaluation of Copy Number Variation Detection Tools from Whole-Exome Sequencing Data Human Mutation How the TP53 Family Proteins TP63 and TP73 Contribute to Tumorigenesis: Regulators and Effectors Human Mutation 2016年最受欢迎的实验室指南 Efficient CRISPR/Cas9‐Based Genome Engineering in Human Pluripotent Stem Cells Current Protocols in Human Genetics RNA‐seq Data: Challenges in and Recommendations for Experimental Design and Analysis Current Protocols in Human Genetics Mouse Genome Editing Using the CRISPR/Cas System Current Protocols in Human Genetics Alternative Splicing Signatures in RNA‐seq Data: Percent Spliced in (PSI) Current Protocols in Human Genetics Chemically Defined Culture and Cardiomyocyte Differentiation of Human Pluripotent Stem Cells Current Protocols in Human Genetics Sequence, Stability, and Structure of G‐Quadruplexes and Their Interactions with Drugs Current Protocols in Nucleic Acid Chemistry Characterization of DNA Structures by Circular Dichroism Current Protocols in Nucleic Acid Chemistry UV Melting of G‐Quadruplexes Current Protocols in Nucleic Acid Chemistry A Status Update of Modified Oligonucleotides for Chemotherapeutics Applications Current Protocols in Nucleic Acid Chemistry
Wiley实验室指南Current Protocols在线讲座 注册地址： http://view6.workcast.net/register?pak=6272104129352788 时间： 2014 年 9 月 30 日（周二） 23:00-0:00 主讲人介绍 ： 邱继辉博士毕业于伦敦的帝国理工学院并获得生物化学博士学位。作为惠康基金奖的博士后研究员留校一年之后，邱博士赴科罗拉多州立大学微生物系任研究员，主要研究方向是麻风病和痨病。到了台湾中央研究院生化研究所后，他开始了自己的独立研究。邱博士现任特聘研究员兼副所长，同时还是中央研究院质谱技术委员会主席，监管几个附属机构的公共质谱设施正常运转。 邱博士的主要研究方向集中在 研发多种串联式质谱分析技术并应用于蛋白质、蛋白后修饰以及糖质体的结构分析、定序与表现差异图谱的鉴定，致力于提升技术平台的高效率、灵敏度与精确度。除此之外，他还积极使用鸟枪蛋白质组学技术来定义多种类型的蛋白质修饰，尤其是半胱氨酸。 主持人： Gwen Taylor， Wiley 高级开发编辑兼Current Protocols在线讲座项目主管 讲座内容介绍： Multiplexing Mass Spectrometry-Based Identification and Quantification of Site-SpecificReversible Cysteine Modifications The identification and quantitative measurement of different forms of protein site-specificreversible cysteine modifications remain technically challenging. Many massspectrometry (MS)-based workflows have been developed, all sharing a commonprinciple of first blocking any free thiol before labeling the specific formsof cysteine modifications that can be reversed by specific reducing agents. Inessence, this switches the reversibly modified cysteine intochemically labeled cysteine more suitable for MS-based detection andquantification. The recent availability of multiplexed iodoTMT, a set ofisobaric mass tags that irreversibly label Cys-thiols, now facilitatesdetermination of the relative degree of specific cysteine modifications underdifferent redox conditions. In this webinar, Dr.Kay-Hooi Khoo (Academia Sinica, Taiwan) will describe howmultiplexed iodoTMT has been used to sequentially and differentially tagS-nitrosylation and S-glutathionylation, allowing their dynamics to bedelineated. Dr. Khoo will answer questions from webinarattendees so be sure to attend and get answers from an expert! 主要学习目标: · Understanding the complexity of cysteinemodifications · Advantages of using iodoTMT in a sequentiallabeling workflow · Potential applications to delineate hypoxia-inducedredox events 讲座主要目标人群： · Researchers using MS-basedproteomics techniques for analysis of post-translational modifications · Scientists interested in using thetechnology to probe redox-mediated cysteine modifications
标签: 实验室指南
