小柯机器人

中国科学院分子植物科学卓越创新中心王二涛等研究人员合作发现，泛素制动器的释放激活水稻中由OsCERK1触发的免疫。该研究于2024年5月15日在线发表于国际一流学术期刊《自然》。

研究人员发现U-box泛素E3连接酶OsCIE1可作为分子制动器抑制水稻中的OsCERK1。在体内平衡期间，OsCIE1泛素化OsCERK1，降低其激酶活性。在微生物相关分子模式几丁质存在的情况下，活性OsCERK1会磷酸化OsCIE1并阻断其E3连接酶活性，从而释放制动器并促进免疫。

OsCIE1 U-框内的丝氨酸磷酸化可阻止其与E2泛素连接酶的相互作用，并起到磷酸化开关的作用。这个磷酸化位点在从植物到动物的E3连接酶中都是保守的。该研究发现了一种配体释放制动器，它能实现动态免疫调节。

据介绍，植物模式识别受体可感知微生物相关分子模式，从而激活免疫信号。模式识别受体激酶CERK1的激活对免疫至关重要，但在没有病原体的情况下严格抑制受体激酶对防止自身免疫至关重要。

附：英文原文

Title: Release of a ubiquitin brake activates OsCERK1-triggered immunity in rice

Author: Wang, Gang, Chen, Xi, Yu, Chengzhi, Shi, Xiaobao, Lan, Wenxian, Gao, Chaofeng, Yang, Jun, Dai, Huiling, Zhang, Xiaowei, Zhang, Huili, Zhao, Boyu, Xie, Qi, Yu, Nan, He, Zuhua, Zhang, Yu, Wang, Ertao

Issue&Volume: 2024-05-15

Abstract: Plant pattern-recognition receptors perceive microorganism-associated molecular patterns to activate immune signalling1,2. Activation of the pattern-recognition receptor kinase CERK1 is essential for immunity, but tight inhibition of receptor kinases in the absence of pathogen is crucial to prevent autoimmunity3,4. Here we find that the U-box ubiquitin E3 ligase OsCIE1 acts as a molecular brake to inhibit OsCERK1 in rice. During homeostasis, OsCIE1 ubiquitinates OsCERK1, reducing its kinase activity. In the presence of the microorganism-associated molecular pattern chitin, active OsCERK1 phosphorylates OsCIE1 and blocks its E3 ligase activity, thus releasing the brake and promoting immunity. Phosphorylation of a serine within the U-box of OsCIE1 prevents its interaction with E2 ubiquitin-conjugating enzymes and serves as a phosphorylation switch. This phosphorylation site is conserved in E3 ligases from plants to animals. Our work identifies a ligand-released brake that enables dynamic immune regulation.

DOI: 10.1038/s41586-024-07418-9

Source: https://www.nature.com/articles/s41586-024-07418-9

Nature：《自然》，创刊于1869年。隶属于施普林格·自然出版集团，最新IF：69.504
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