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小鼠新皮质顶端祖细胞在发育过程中具有时间可塑性
2019-08-29 15:19

瑞士日内瓦大学Denis Jabaudon研究组发现小鼠新皮质顶端祖细胞在发育过程中具有时间可塑性。该研究2019年8月28日在线发表于《自然》。

研究人员专门分离了顶端祖细胞和中间祖细胞,并通过异源移植到年轻胚胎中,将各自神经元后代命运进行映射。研究人员发现顶端祖细胞在时间上是可塑的,并且当其暴露于早期发育环境时(通过感知细胞外Wnt的动态变化),这些细胞可以重新恢复之前的分子、电生理和神经原性状态。相比之下,中间祖细胞是缺乏这种逆行命运可塑性的祖细胞。这些发现确定了新皮质祖细胞时间可塑性的多样性,并揭示了一些细胞亚型可以从其正常的时间发育中脱离,重新回到之前的发育状态。

据介绍,新皮层中存在的兴奋性神经元的不同亚型来自位于室区的顶端祖细胞。在皮质发生期间,顶端祖细胞依次产生深层神经元,然后直接或通过中间祖细胞的产生来产生表层神经元。神经源性细胞命运进展是否必然意味着受限于单个祖先类型命运还不清楚。

附:英文原文

Title: Temporal plasticity of apical progenitors in the developing mouse neocortex

Author: Polina Oberst, Sabine Fivre, Natalia Baumann, Cristina Concetti, Giorgia Bartolini, Denis Jabaudon

Issue&Volume: 2019-08-28

Abstract: The diverse subtypes of excitatory neurons that populate the neocortex are born from apical progenitors located in the ventricular zone. During corticogenesis, apical progenitors sequentially generate deep-layer neurons followed by superficial-layer neurons directly or via the generation of intermediate progenitors. Whether neurogenic fate progression necessarily implies fate restriction in single progenitor types is unknown. Here we specifically isolated apical progenitors and intermediate progenitors, and fate-mapped their respective neuronal progeny following heterochronic transplantation into younger embryos. We find that apical progenitors are temporally plastic and can re-enter past molecular, electrophysiological and neurogenic states when exposed to an earlier-stage environment by sensing dynamic changes in extracellular Wnt. By contrast, intermediate progenitors are committed progenitors that lack such retrograde fate plasticity. These findings identify a diversity in the temporal plasticity of neocortical progenitors, revealing that some subtypes of cells can be untethered from their normal temporal progression to re-enter past developmental states.

DOI: 10.1038/s41586-019-1515-6

Source: https://www.nature.com/articles/s41586-019-1515-6

Nature:《自然》,创刊于1869年。隶属于施普林格·自然出版集团,最新IF:69.504
官方网址:http://www.nature.com/
投稿链接:http://www.nature.com/authors/submit_manuscript.html


本期文章:《自然》:Online/在线发表

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