小柯机器人

近日，美国范德比尔特大学医学中心Ivelin S. Georgiev及其研究组研发的新技术，实现对B细胞受体（BCR）序列针对抗原特异性的高通量测序。2019年11月28日，国际知名学术期刊《细胞》在线发表了这一成果。

研究人员提出了LIBRA-seq（通过测序将B细胞受体与抗原特异性连接）的技术，其可将成对的重链和轻链BCR序列与其同源抗原特异性进行高通量映射。B细胞与一组DNA条形码的抗原混合，这样抗原条形码和BCR序列均可通过单细胞下一代测序回收。使用LIBRA-seq，研究人员绘制了来自两个HIV感染者的数千个B细胞的抗原特异性。

这些结果证实了对许多HIV和流感特异性抗体的预测特异性，包括已知和新型的广泛中和抗体。LIBRA-seq将成为针对多种抗原靶标的抗体发现和疫苗开发工作的不可或缺的工具。

据悉，BCR测序是用于研究对感染和疫苗接种的免疫反应的强大工具，但它提供的BCR抗原特异性信息有限。

附：英文原文

Title: High-Throughput Mapping of B Cell Receptor Sequences to Antigen Specificity

Author: Ian Setliff, Andrea R. Shiakolas, Kelsey A. Pilewski, Amyn A. Murji, Rutendo E. Mapengo, Katarzyna Janowska, Simone Richardson, Charissa Oosthuysen, Nagarajan Raju, Larance Ronsard, Masaru Kanekiyo, Juliana S. Qin, Kevin J. Kramer, Allison R. Greenplate, Wyatt J. McDonnell, Barney S. Graham, Mark Connors, Daniel Lingwood, Priyamvada Acharya, Lynn Morris, Ivelin S. Georgiev

Issue&Volume: November 28, 2019

Abstract: B cell receptor (BCR) sequencing is a powerful tool for interrogating immune responsesto infection and vaccination, but it provides limited information about the antigenspecificity of the sequenced BCRs. Here, we present LIBRA-seq (linking B cell receptorto antigen specificity through sequencing), a technology for high-throughput mappingof paired heavy- and light-chain BCR sequences to their cognate antigen specificities.B cells are mixed with a panel of DNA-barcoded antigens so that both the antigen barcode(s)and BCR sequence are recovered via single-cell next-generation sequencing. Using LIBRA-seq,we mapped the antigen specificity of thousands of B cells from two HIV-infected subjects.The predicted specificities were confirmed for a number of HIV- and influenza-specificantibodies, including known and novel broadly neutralizing antibodies. LIBRA-seq willbe an integral tool for antibody discovery and vaccine development efforts againsta wide range of antigen targets.

DOI: 10.1016/j.cell.2019.11.003

Source: https://www.cell.com/cell/fulltext/S0092-8674(19)31224-3