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中国科学家绘制3D培养的人原肠运动前胚胎发育图谱
2019-12-17 16:05

近日,昆明理工大学李天晴、季维智等研究人员合作描绘了3D培养的人类原肠运动前胚胎的发育图谱。该项研究成果2019年12月13日在线发表在《自然》上。

研究人员报告了一个三维(3D)囊胚培养系统,它可以通过原条(PSA)使人类囊胚发育。这些3D胚胎模仿体内的发育标志和3D结构,包括胚盘、羊膜、基底膜、初级和灵长类独特的次级卵黄囊、前后极性形成和PSA。使用单细胞转录组分析,研究人员描述了上外胚层、原始内胚层和滋养细胞分离的本体和调控网络。与外胚层相比,羊膜上皮细胞显示出独特和特征性的表型。着床后,特定的途径和转录因子触发细胞滋养细胞、绒毛外滋养细胞和合体滋养细胞的分化。外胚层在着床时经历多能性转换并维持其转录组,直到由不同的多能性因子驱动的PSA产生。使用这一3D培养方法,研究人员阐明了人类胚胎发生过程中的分子和形态发生的发育图谱。

据了解,详细的人类妊娠前胚胎发育(包括空间自组织和细胞类型个体发育)的知识仍然受到可用的二维技术平台的限制,因为这些技术无法反映体内条件。

附:英文原文

Title: A developmental landscape of 3D-cultured human pre-gastrulation embryos

Author: Lifeng Xiang, Yu Yin, Yun Zheng, Yanping Ma, Yonggang Li, Zhigang Zhao, Junqiang Guo, Zongyong Ai, Yuyu Niu, Kui Duan, Jingjing He, Shuchao Ren, Dan Wu, Yun Bai, Zhouchun Shang, Xi Dai, Weizhi Ji, Tianqing Li

Issue&Volume: 2019-12-12

Abstract: Knowledge detailing human pre-gastrulation embryonic development including spatial self-organization and cell type ontogeny remains limited by available two-dimensional technological platforms1,2 that do not recapitulate the in vivo condition3–5. Here, we report a three-dimensional (3D) blastocyst-culture system, which enables human blastocyst development through primitive streak anlage (PSA). These 3D embryos mimic in vivo developmental landmarks and 3D architectures, including embryonic disc, amnion, basement membrane, primary and primate unique secondary yolk sac, anterior-posterior polarity formation and PSA. Using single-cell transcriptome profiling, we delineate ontology and regulatory networks underlying epiblast, primitive endoderm and trophoblast segregation. Compared to epiblast, the amniotic epithelium shows unique and characteristic phenotypes. After implantation, specific pathways and transcription factors trigger differentiation of cytotrophoblasts, extravillous cytotrophoblasts and syncytiotrophoblasts. Epiblasts undergo pluripotency transition upon implantation and maintain its transcriptome until PSA generation driven by different pluripotent factors. Using our 3D-culture approach, our findings elucidate the molecular and morphogenetic developmental landscape during human embryogenesis.

DOI: 10.1038/s41586-019-1875-y

Source: https://www.nature.com/articles/s41586-019-1875-y

Nature:《自然》,创刊于1869年。隶属于施普林格·自然出版集团,最新IF:69.504
官方网址:http://www.nature.com/
投稿链接:http://www.nature.com/authors/submit_manuscript.html


本期文章:《自然》:Online/在线发表

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