德国马克斯·普朗克生物物理化学研究所Stefan W. Hell研究团队开发出MINFLUX纳米显微镜，可实现细胞中3D多色的纳米分辨率成像。相关论文2020年1月13日在线发表在《自然—方法学》上。
通过使用甜甜圈形激发光束定位单个可切换荧光团，MINFLUX纳米显微镜可以为固定细胞和活细胞中的结构提供1至3 nm的分辨率。以探测光束最低值的方式迭代地接近每个荧光团，可促进此过程，并使分辨率在可伸缩的视场上基本均匀且各向同性。哺乳动物细胞核孔复合物的MINFLUX成像显示，该真正的纳米级分辨率是在三个维度和两个颜色通道中获得的。依靠比标准相机定位技术更少检测到的光子，MINFLUX纳米技术有望开创固定细胞和活细胞中蛋白质复合物成像以及分布的新篇章。
据介绍，生物超分辨率荧光显微镜的最终目标是在荧光标记的尺寸范围内提供三维分辨率。
附：英文原文
Title: MINFLUX nanoscopy delivers 3D multicolor nanometer resolution in cells
Author: Klaus C. Gwosch, Jasmin K. Pape, Francisco Balzarotti, Philipp Hoess, Jan Ellenberg, Jonas Ries, Stefan W. Hell
Issue&Volume: 2020-01-13
Abstract: The ultimate goal of biological super-resolution fluorescence microscopy is to provide three-dimensional resolution at the size scale of a fluorescent marker. Here we show that by localizing individual switchable fluorophores with a probing donut-shaped excitation beam, MINFLUX nanoscopy can provide resolutions in the range of 1 to 3nm for structures in fixed and living cells. This progress has been facilitated by approaching each fluorophore iteratively with the probing-donut minimum, making the resolution essentially uniform and isotropic over scalable fields of view. MINFLUX imaging of nuclear pore complexes of a mammalian cell shows that this true nanometer-scale resolution is obtained in three dimensions and in two color channels. Relying on fewer detected photons than standard camera-based localization, MINFLUX nanoscopy is poised to open a new chapter in the imaging of protein complexes and distributions in fixed and living cells.
DOI: 10.1038/s41592-019-0688-0
Source: https://www.nature.com/articles/s41592-019-0688-0
Nature Methods：《自然—方法学》，创刊于2004年。隶属于施普林格·自然出版集团，最新IF：47.99
官方网址：https://www.nature.com/nmeth/
投稿链接：https://mts-nmeth.nature.com/cgi-bin/main.plex
本期文章：《自然—方法学》：Online/在线发表
