小柯机器人

中国科学家解码人类海马体发育
2020-01-17 00:01

中国科学院生物物理研究所王晓群团队与北京师范大学吴倩团队合作解码了人类海马体的发育。这一研究成果2020年1月15日在线发表于国际学术期刊《自然》。

研究人员使用单细胞RNA测序,并通过转座酶可及性染色质测序(ATAC–seq)分析来揭示发育中的人类海马体的细胞类型、细胞谱系、分子特征和转录调控。利用在妊娠第16–27周时来自人类海马区的30416个细胞的转录组,研究人员鉴定了47种细胞亚型及其发育轨迹。研究人员还确定了PAX6+和HOPX+海马祖细胞的迁移途径和细胞谱系,以及CA1、CA3和齿状回神经元的区域标记。多种基因组数据已发现齿状回标记物PROX1的转录调控网络。研究人员还说明了发育中的人类前额叶皮层和海马体中的空间特异性基因表达。妊娠第16-20周人类海马体的分子特征与出生后0-5天的小鼠相似,并揭示了这两种物种之间的基因表达差异。灵长类特定基因NBPF1的瞬时表达导致小鼠海马区中PROX1+细胞的显著增加。这些数据为了解人类海马体的发育提供了蓝图,也为研究相关疾病提供了工具。
 
据介绍,海马体是人脑边缘系统的重要组成部分,在空间导航以及从短期记忆到长期记忆的信息整合中具有至关重要的作用。
 
附:英文原文

Title: Decoding the development of the human hippocampus

Author: Suijuan Zhong, Wenyu Ding, Le Sun, Yufeng Lu, Hao Dong, Xiaoying Fan, Zeyuan Liu, Ruiguo Chen, Shu Zhang, Qiang Ma, Fuchou Tang, Qian Wu, Xiaoqun Wang

Issue&Volume: 2020-01-15

Abstract: The hippocampus is an important part of the limbic system in the human brain that has essential roles in spatial navigation and the consolidation of information from short-term memory to long-term memory1,2. Here we use single-cell RNA sequencing and assay for transposase-accessible chromatin using sequencing (ATAC–seq) analysis to illustrate the cell types, cell linage, molecular features and transcriptional regulation of the developing human hippocampus. Using the transcriptomes of 30,416 cells from the human hippocampus at gestational weeks 16–27, we identify 47 cell subtypes and their developmental trajectories. We also identify the migrating paths and cell lineages of PAX6+ and HOPX+ hippocampal progenitors, and regional markers of CA1, CA3 and dentate gyrus neurons. Multiomic data have uncovered transcriptional regulatory networks of the dentate gyrus marker PROX1. We also illustrate spatially specific gene expression in the developing human prefrontal cortex and hippocampus. The molecular features of the human hippocampus at gestational weeks 16–20 are similar to those of the mouse at postnatal days 0–5 and reveal gene expression differences between the two species. Transient expression of the primate-specific gene NBPF1 leads to a marked increase in PROX1+ cells in the mouse hippocampus. These data provides a blueprint for understanding human hippocampal development and a tool for investigating related diseases.

DOI: 10.1038/s41586-019-1917-5

Source: https://www.nature.com/articles/s41586-019-1917-5

Nature:《自然》,创刊于1869年。隶属于施普林格·自然出版集团,最新IF:43.07
官方网址:http://www.nature.com/
投稿链接:http://www.nature.com/authors/submit_manuscript.html


本期文章:《自然》:Online/在线发表

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