2020年3月4日,荷兰代尔夫特理工大学Cees Dekker课题组在《自然》杂志在线发表论文,揭示了负责DNA环挤出的集缩素复合物可以穿过彼此。
Title: DNA-loop extruding condensin complexes can traverse one another
Author: Eugene Kim, Jacob Kerssemakers, Indra A. Shaltiel, Christian H. Haering, Cees Dekker
Issue&Volume: 2020-03-04
Abstract: Condensin, a key component of the structure maintenance of chromosome (SMC) protein complexes, has recently been shown to be a motor that extrudes loops of DNA1. It remains unclear, however, how condensin complexes work together to collectively package DNA into chromosomes. Here we use time-lapse single-molecule visualization to study mutual interactions between two DNA-loop-extruding yeast condensins. We find that these motor proteins, which, individually, extrude DNA in one direction only are able to dynamically change each other’s DNA loop sizes, even when far apart. When they are in close proximity, condensin complexes are able to traverse each other and form a loop structure, which we term a Z-loop—three double-stranded DNA helices aligned in parallel with one condensin at each edge. Z-loops can fill gaps left by single loops and can form symmetric dimer motors that pull in DNA from both sides. These findings indicate that condensin may achieve chromosomal compaction using a variety of looping structures.
DOI: 10.1038/s41586-020-2067-5
Source: https://www.nature.com/articles/s41586-020-2067-5
Nature:《自然》,创刊于1869年。隶属于施普林格·自然出版集团,最新IF:69.504
官方网址:http://www.nature.com/
投稿链接:http://www.nature.com/authors/submit_manuscript.html
本期文章:《自然》:Online/在线发表