小柯机器人

美国德克萨斯大学Edward M. Marcotte研究组绘制出泛植物蛋白复合物图谱，从而揭示了高度的保守性和新的组装体。相关论文于2020年3月18日在线发表于《细胞》杂志。

研究人员从13种具有科学和农业重要性的植物中系统地鉴定了蛋白质复合物，从而大大拓展了植物中已知的稳定蛋白质复合物。通过使用共分馏质谱，研究人员验证了已知的复合物，确认了植物中预测的复合物，并鉴定了以往未知的相互作用，这些相互作用在绿色植物进化中保守存在了11亿余年。几种新的复合物参与春化和病原体防御，这对农业至关重要。研究人员还观察到了动物复合物在植物中的类似物，这些具有不同的分子组装，包括兆达尔顿级的tRNA多合成酶复合物。研究人员得到的图谱提供了不同植物细胞中保守、稳定蛋白质复合物的跨物种视角，并提供了可用于解释植物遗传学和突变表型的机制与生化框架。

据介绍，植物是全球生态和经济系统的基础，但大多数植物蛋白仍未表征。蛋白质相互作用网络通常提示了蛋白质的功能，并开辟了表征基因和蛋白质的新途径。

附：英文原文

Title: A Pan-plant Protein Complex Map Reveals Deep Conservation and Novel Assemblies

Author: Claire D. McWhite, Ophelia Papoulas, Kevin Drew, Rachael M. Cox, Viviana June, Oliver Xiaoou Dong, Taejoon Kwon, Cuihong Wan, Mari L. Salmi, Stanley J. Roux, Karen S. Browning, Z. Jeffrey Chen, Pamela C. Ronald, Edward M. Marcotte

Issue&Volume: 2020-03-18

Abstract: Plants are foundational for global ecological and economic systems, but most plantproteins remain uncharacterized. Protein interaction networks often suggest proteinfunctions and open new avenues to characterize genes and proteins. We therefore systematicallydetermined protein complexes from 13 plant species of scientific and agriculturalimportance, greatly expanding the known repertoire of stable protein complexes inplants. By using co-fractionation mass spectrometry, we recovered known complexes,confirmed complexes predicted to occur in plants, and identified previously unknowninteractions conserved over 1.1 billion years of green plant evolution. Several novelcomplexes are involved in vernalization and pathogen defense, traits critical foragriculture. We also observed plant analogs of animal complexes with distinct molecularassemblies, including a megadalton-scale tRNA multi-synthetase complex. The resultingmap offers a cross-species view of conserved, stable protein assemblies shared acrossplant cells and provides a mechanistic, biochemical framework for interpreting plantgenetics and mutant phenotypes.

DOI: 10.1016/j.cell.2020.02.049

Source: https://www.cell.com/cell/fulltext/S0092-8674(20)30226-9