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piRNA可控制伊蚊的胚胎发育
2020-04-02 17:09

荷兰拉德布德分子生命科学研究所Ronald P. van Rij研究组发现卫星重复序列来源的piRNA控制伊蚊的胚胎发育。202041日出版的《自然》杂志发表了这项成果。

他们显示蚊子埃及伊蚊中的卫星重复序列通过两个PIWI相互作用RNApiRNA)的表达促进序列特异性基因沉默。尽管卫星重复序列和piRNA序列通常进化得非常迅速,但该基因座已保存了约2亿年,这表明它在蚊子生物学中具有重要作用。产卵后不久就开始产生piRNA,而更丰富的piRNA的失活导致无法降解合子和发育停滞中母体沉积的转录本。他们的研究结果揭示了一种必不可少的机制,卫星重复序列调控整体基因表达,是通过对胚胎发育至关重要的piRNA介导的基因沉默。

据介绍,串联重复序列,如不同种类的卫星重复序列,占据了真核染色体的大部分位置,大部分位于着丝粒、臂间着丝粒、端粒和亚端粒区域。然而,一些元件位于整个基因组的常染色体区域,并被认为通过调节局部染色质结构来顺式调节基因表达,或通过自重复序列来源的转录本来反式调节基因表达。

附:英文原文

Title: A satellite repeat-derived piRNA controls embryonic development of Aedes

Author: Rebecca Halbach, Pascal Miesen, Joep Joosten, Ezgi Takpr, Inge Rondeel, Bas Pennings, Chantal B. F. Vogels, Sarah H. Merkling, Constantianus J. Koenraadt, Louis Lambrechts, Ronald P. van Rij

Issue&Volume: 2020-04-01

Abstract: Tandem repeat elements such as the diverse class of satellite repeats occupy large parts of eukaryotic chromosomes, mostly at centromeric, pericentromeric, telomeric and subtelomeric regions1. However, some elements are located in euchromatic regions throughout the genome and have been hypothesized to regulate gene expression in cis by modulating local chromatin structure, or in trans via transcripts derived from the repeats2,3,4. Here we show that a satellite repeat in the mosquito Aedes aegypti promotes sequence-specific gene silencing via the expression of two PIWI-interacting RNAs (piRNAs). Whereas satellite repeats and piRNA sequences generally evolve extremely quickly5,6,7, this locus was conserved for approximately 200 million years, suggesting that it has a central function in mosquito biology. piRNA production commenced shortly after egg laying, and inactivation of the more abundant piRNA resulted in failure to degrade maternally deposited transcripts in the zygote and developmental arrest. Our results reveal a mechanism by which satellite repeats regulate global gene expression in trans via piRNA-mediated gene silencing that is essential for embryonic development.

DOI: 10.1038/s41586-020-2159-2

Source: https://www.nature.com/articles/s41586-020-2159-2

Nature:《自然》,创刊于1869年。隶属于施普林格·自然出版集团,最新IF:43.07
官方网址:http://www.nature.com/
投稿链接:http://www.nature.com/authors/submit_manuscript.html


本期文章:《自然》:Online/在线发表

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