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母乳喂养能够调节婴儿肠道病毒组的逐渐产生
2020-04-17 20:17

美国宾夕法尼亚大学Frederic D. Bushman研究团队的一项最新工作发现,母乳喂养可调节新生儿肠道病毒组的逐步产生。相关论文于2020年4月15日在线发表于《自然》。

健康的人类新生儿的肠道通常在出生时就没有病毒,但很快就会定植,这在某些情况下会导致胃肠道疾病。
 
研究人员发现,新生儿病毒群落的产生是通过不同的步骤进行的。从婴儿胎粪或早期粪便样品中纯化的病毒样颗粒荧光染色结果显示出很少或没有颗粒,但是到第一个月时,颗粒数量增加到每克109,这些数量似乎在整个生命中都持续存在。
 
研究人员使用霰弹枪宏基因组测序对富含病毒的样品和整个微生物群落进行了测试,并研究了这些病毒种群的起源,然后进行了靶向微生物学分析。
 
结果表明,出生后早期,先驱细菌在婴儿肠道内定殖,由这些细菌诱导的噬菌体在一个月后便成为病毒样颗粒的主要种群。到第四个月时,在人细胞中复制的可识别病毒变得更加突出。与仅部分或全部以母乳喂养的婴儿粪便样本相比,完全以配方奶粉喂养的婴儿粪便样本中的多种人类病毒含量更高,与此同时,有报道称母乳可以预防病毒感染。噬菌体群体也根据婴儿是否母乳喂养而有所不同。
 
研究人员发现,婴儿肠道的定殖是逐步进行的,首先主要是由先驱细菌诱导的温和噬菌体,然后是在人细胞中复制的病毒。第二阶段会通过母乳喂养进行调节。
 
附:英文原文

Title: The stepwise assembly of the neonatal virome is modulated by breastfeeding

Author: Guanxiang Liang, Chunyu Zhao, Huanjia Zhang, Lisa Mattei, Scott Sherrill-Mix, Kyle Bittinger, Lyanna R. Kessler, Gary D. Wu, Robert N. Baldassano, Patricia DeRusso, Eileen Ford, Michal A. Elovitz, Matthew S. Kelly, Mohamed Z. Patel, Tiny Mazhani, Jeffrey S. Gerber, Andrea Kelly, Babette S. Zemel, Frederic D. Bushman

Issue&Volume: 2020-04-15

Abstract: The gut of healthy human neonates is usually devoid of viruses at birth, but quickly becomes colonized, which—in some cases—leads to gastrointestinal disorders1,2,3,4. Here we show that the assembly of the viral community in neonates takes place in distinct steps. Fluorescent staining of virus-like particles purified from infant meconium or early stool samples shows few or no particles, but by one month of life particle numbers increase to 109 per gram, and these numbers seem to persist throughout life5,6,7. We investigated the origin of these viral populations using shotgun metagenomic sequencing of virus-enriched preparations and whole microbial communities, followed by targeted microbiological analyses. Results indicate that, early after birth, pioneer bacteria colonize the infant gut and by one month prophages induced from these bacteria provide the predominant population of virus-like particles. By four months of life, identifiable viruses that replicate in human cells become more prominent. Multiple human viruses were more abundant in stool samples from babies who were exclusively fed on formula milk compared with those fed partially or fully on breast milk, paralleling reports that breast milk can be protective against viral infections8,9,10. Bacteriophage populations also differed depending on whether or not the infant was breastfed. We show that the colonization of the infant gut is stepwise, first mainly by temperate bacteriophages induced from pioneer bacteria, and later by viruses that replicate in human cells; this second phase is modulated by breastfeeding.

DOI: 10.1038/s41586-020-2192-1

Source: https://www.nature.com/articles/s41586-020-2192-1

Nature:《自然》,创刊于1869年。隶属于施普林格·自然出版集团,最新IF:43.07
官方网址:http://www.nature.com/
投稿链接:http://www.nature.com/authors/submit_manuscript.html


本期文章:《自然》:Online/在线发表

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