小柯机器人

韩国基础科学研究所(IBS) Hyeshik Chang、V. Narry Kim等研究人员合作报道了新冠病毒（SARS-CoV-2）的转录组结构。这一研究成果于2020年4月23日在线发表于《细胞》。

Title: The Architecture of SARS-CoV-2 Transcriptome

Author: Dongwan Kim, Joo-Yeon Lee, Jeong-Sun Yang, Jun Won Kim, V. Narry Kim, Hyeshik Chang

Issue&Volume: 2020-04-23

Abstract: SARS-CoV-2 is a betacoronavirus responsible for the COVID-19 pandemic. Although the SARS-CoV-2 genome was reported recently, its transcriptomic architecture is unknown. Utilizing two complementary sequencing techniques, we present a high-resolution map of the SARS-CoV-2 transcriptome and epitranscriptome. DNA nanoball sequencing shows that the transcriptome is highly complex owing to numerous discontinuous transcription events. In addition to the canonical genomic and 9 subgenomic RNAs, SARS-CoV-2 produces transcripts encoding unknown ORFs with fusion, deletion, and/or frameshift. Using nanopore direct RNA sequencing, we further find at least 41 RNA modification sites on viral transcripts, with the most frequent motif, AAGAA. Modified RNAs have shorter poly(A) tails than unmodified RNAs, suggesting a link between the modification and the 3′ tail. Functional investigation of the unknown transcripts and RNA modifications discovered in this study will open new directions to our understanding of the life cycle and pathogenicity of SARS-CoV-2.

DOI: 10.1016/j.cell.2020.04.011

Source: https://www.cell.com/cell/fulltext/S0092-8674(20)30406-2