小柯机器人

近日，美国普渡大学Fang Huang、Sarah Calve、印第安纳大学Gary E. Landreth等研究人员，合作研发了可用于全细胞和组织观察的三维纳米显微镜。这一研究成果于2020年5月4日发表在《自然—方法学》上。

Title: Three-dimensional nanoscopy of whole cells and tissues with in situ point spread function retrieval

Author: Fan Xu, Donghan Ma, Kathryn P. MacPherson, Sheng Liu, Ye Bu, Yu Wang, Yu Tang, Cheng Bi, Tim Kwok, Alexander A. Chubykin, Peng Yin, Sarah Calve, Gary E. Landreth, Fang Huang

Issue&Volume: 2020-05-04

Abstract: Single-molecule localization microscopy is a powerful tool for visualizing subcellular structures, interactions and protein functions in biological research. However, inhomogeneous refractive indices inside cells and tissues distort the fluorescent signal emitted from single-molecule probes, which rapidly degrades resolution with increasing depth. We propose a method that enables the construction of an in situ 3D response of single emitters directly from single-molecule blinking datasets, and therefore allows their locations to be pinpointed with precision that achieves the Cramér-Rao lower bound and uncompromised fidelity. We demonstrate this method, named in situ PSF retrieval (INSPR), across a range of cellular and tissue architectures, from mitochondrial networks and nuclear pores in mammalian cells to amyloid-β plaques and dendrites in brain tissues and elastic fibers in developing cartilage of mice. This advancement expands the routine applicability of super-resolution microscopy from selected cellular targets near coverslips to intra- and extracellular targets deep inside tissues.

DOI: 10.1038/s41592-020-0816-x

Source: https://www.nature.com/articles/s41592-020-0816-x

Nature Methods：《自然—方法学》，创刊于2004年。隶属于施普林格·自然出版集团，最新IF：47.99
官方网址：https://www.nature.com/nmeth/
投稿链接：https://mts-nmeth.nature.com/cgi-bin/main.plex