小柯机器人

中国科学家建立新冠病毒仓鼠感染模型
2020-05-15 11:01

香港大学Hui-Ling Yen研究团队建立了新冠病毒(SARS-CoV-2)仓鼠感染模型。这一研究成果于2020年5月14日在线发表在《自然》上。

研究人员报道了SARS-CoV-2在金色叙利亚仓鼠中的发病机理和传染性。免疫组织化学表明,接种后(dpi)第2天和第5天在鼻粘膜,支气管上皮细胞和肺巩固区域中存在病毒抗原,随后在7 dpi时迅速清除病毒并引起肺细胞增生。在十二指肠上皮细胞中也发现了病毒抗原,粪便中检测到病毒RNA。
 
值得注意的是,SARS-CoV-2通过直接接触和通过气溶胶从感染的仓鼠有效地传播到未感染的仓鼠。在污染的笼子中通过污染物传播的效率较低。尽管在感染的仓鼠鼻洗液中连续14天连续检测到病毒RNA,但可传播时间短,并且与感染性病毒的检测相关,而与病毒RNA无关。人为感染和自然感染的仓鼠表现出明显的体重减轻,所有康复的动物均检测到中和抗体。这些结果表明,金色叙利亚仓鼠中的SARS-CoV-2感染类似于人类的轻度感染。
 
附:英文原文

Title: Pathogenesis and transmission of SARS-CoV-2 in golden hamsters

Author: Sin Fun Sia, Li-Meng Yan, Alex W. H. Chin, Kevin Fung, Ka-Tim Choy, Alvina Y. L. Wong, Prathanporn Kaewpreedee, Ranawaka A. P. M. Perera, Leo L. M. Poon, John M. Nicholls, Malik Peiris, Hui-Ling Yen

Issue&Volume: 2020-05-14

Abstract: SARS-CoV-2, a novel coronavirus with high nucleotide identity to SARS-CoV and SARS-related coronaviruses detected in horseshoe bats, has spread across the world and impacted global healthcare systems and economy1,2. A suitable small animal model is needed to support vaccine and therapy development. We report the pathogenesis and transmissibility of the SARS-CoV-2 in golden Syrian hamsters. Immunohistochemistry demonstrated viral antigens in nasal mucosa, bronchial epithelial cells, and in areas of lung consolidation on days 2 and 5 post-inoculation (dpi), followed by rapid viral clearance and pneumocyte hyperplasia on 7 dpi. Viral antigen was also found in the duodenum epithelial cells with viral RNA detected in feces. Notably, SARS-CoV-2 transmitted efficiently from inoculated hamsters to nave hamsters by direct contact and via aerosols. Transmission via fomites in soiled cages was less efficient. Although viral RNA was continuously detected in the nasal washes of inoculated hamsters for 14 days, the communicable period was short and correlated with the detection of infectious virus but not viral RNA. Inoculated and naturally-infected hamsters showed apparent weight loss, and all animals recovered with the detection of neutralizing antibodies. Our results suggest that SARS-CoV-2 infection in golden Syrian hamsters resemble features found in humans with mild infections.

DOI: 10.1038/s41586-020-2342-5

Source:https://www.nature.com/articles/s41586-020-2342-5

Nature:《自然》,创刊于1869年。隶属于施普林格·自然出版集团,最新IF:43.07
官方网址:http://www.nature.com/
投稿链接:http://www.nature.com/authors/submit_manuscript.html


本期文章:《自然》:Online/在线发表

分享到:

0